Abstract：
:After our promising results from prophylactic and therapeutic study (i.p. route) with the radio-attenuated Leishmania donovani parasites against experimental murine visceral leishmaniasis, we prompted to check their therapeutic efficacy through i.m route. BALB/c mice were infected with highly virulent L. donovani parasites. After 75 days, mice were treated with gamma (γ)-irradiated parasites. A second therapeutic immunization was given after 15 days of first immunization. The protection against kala-azar was estimated with the reduction of Leishman-Donovan unit from spleen and liver that scored up to 80% and 93%, respectively, while a twofold increase in nitric oxide (NO) and reactive oxygen species (ROS) productions has been observed in the immunized groups of animals. These groups of mice also showed disease regression by skewing Th2 cytokines (IL-10) towards Th1 cytokine (IFN-γ) bias along with the increased generation of NO and ROS, while the infected control group of mice without such treatment surrendered to the disease. Establishment of Th1 ambience in the treated groups has also been supported from the measured antileishmanial antibody IgG subsets (IgG2a and IgG1) with higher anti-soluble Leishmania antigen-specific IgG2a titer. As seen in our previous studies, doses of attenuation by γ-radiation should be taken into serious consideration. Attenuation of parasites at 50 Gy of absorbed dose of gamma rays has not worked well. Thus, therapeutic use of L. donovani parasites radio-attenuated at particular doses can be exploited as a promising vaccine agent. Absence of any adjuvant may increase its acceptability as vaccine candidate further.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Datta S,Manna M,Khanra S,Ghosh M,Bhar R,Chakraborty A,Roy Sdoi
10.1007/s00436-012-2847-4subject
Has Abstractpub_date
2012-07-01 00:00:00pages
361-9issue
1eissn
0932-0113issn
1432-1955journal_volume
111pub_type
杂志文章abstract：:During investigations of fish parasites in the Neotropics (including the state of Veracruz and the Yucatán Peninsula in the Gulf of Mexico, the Chautengo Lagoon on the Pacific coast of the state of Guerrero in Mexico, and Lake Gatun in the Panama Canal), three monogenoidean (Dactylogyridae) species were found parasiti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1442-9
更新日期：2009-09-01 00:00:00
abstract：:The prevalence data of Leishmania infantum infection in cats are characterized by a large variability mainly attributed to the differences in diagnostic techniques. In the absence of consensus about the method of choice for diagnosing feline leishmaniosis, the performance of a new immunofluorescence antibody test (IFA...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06627-1
更新日期：2020-04-01 00:00:00
abstract：:Toxoplasma gondii is an important zoonotic protozoan of the phylum Apicomplexa that can infect nearly all warm-blooded animals. The parasite can exist as the interconvertible tachyzoite or bradyzoite forms, leading to acute or latent infection, respectively. No drug has been reported to penetrate the cyst wall and red...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06722-3
更新日期：2020-07-01 00:00:00
abstract：:The complete internal transcribed spacer 1 (ITS1), 5.8S rDNA, and ITS2 region of the ribosomal DNA and a 390-bp region of the 16S rDNA gene from five taxa belonging to Rhinonyssus (Rhinonyssus vanellus, Rhinonyssus tringae, Rhinonyssus neglectus, Rhinonyssus echinipes from Kentish plover, and Rhinonyssus echinipes fro...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0356-z
更新日期：2007-04-01 00:00:00
abstract：:The prevalence and abundance of infections with haemoparasites were studied over a 4-year period in Microtus arvalis (common vole, n =321) sampled from fallow grassland sites in north-eastern Poland. Total species richness was five (prevalence= Haemobartonella sp. 63.9%, Bartonella spp. 27.7%, Babesia microti 9.0%, Tr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1040-1
更新日期：2004-03-01 00:00:00
abstract：:A retrospective study was carried out on the experimental infections of Lymnaea truncatula with Fasciola hepatica performed over the last 20 years to determine if the populations of snails living in highland or lowland countries had the same ability to sustain trematode larval development. The six highland populations...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-001-0542-y
更新日期：2002-04-01 00:00:00
abstract：:We report on serine protease activity in cercarial secretions (CSs) from the bird parasite Trichobilharzia ocellata. Using a colorigenic substrate, the biochemical properties of this enzyme were studied and its activity was compared to the homologous one in CSs from the human parasite Schistosoma mansoni. The specific...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0597-4
更新日期：2002-06-01 00:00:00
abstract：:Sarcocystis sarcocysts are common in many species of domestic and wild animals. Here, we report sarcocystosis in muscles from 91 free range elk (Cervus elaphus) from Pennsylvania, USA, tested by histopathology, transmission electron microscopy (TEM), and DNA sequencing. Sarcocysts were detected in hematoxylin and eosi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6024-2
更新日期：2018-10-01 00:00:00
abstract：:Helminths are one of the major health problems of working donkeys, often with heavy worm burden and contributing to their early demise and/or reduction in their work output. Cross-sectional and longitudinal studies were conducted to investigate the current infection prevalence and level of strongyles infection donkeys...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5485-z
更新日期：2017-07-01 00:00:00
abstract：:Two species of Babesia were isolated from naturally infected sheep in the eastern part of Gansu province. One of the species was identified as B. ovis, while the another one seems as yet to be an unidentified Babesia species. The latter could be transmitted transovarially by Haemaphysalis longicornis. It was shown tha...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-001-0563-6
更新日期：2002-05-01 00:00:00
abstract：:Mosquito bites can elicit dermal hypersensitivity reactions, but little is known about the chemotactic factors for host leukocytes in mosquito saliva. In this study, we determined that saliva from a malarial vector mosquito, Anopheles stephensi, possesses intense neutrophil chemotactic activity. In contrast, the midgu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000355
更新日期：2001-05-01 00:00:00
abstract：:Multiple schistosome and soil-transmitted nematode infections are frequently reported in human populations living in tropical areas of developing countries. In addition to exposure factors, the host immune response plays an important role in helminth control and morbidity in hosts with multiple infections; however, th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4706-6
更新日期：2015-12-01 00:00:00
abstract：:Spermiogenesis and ultrastructure of the spermatozoon of two bothriocephalidean cestodes, Oncodiscus sauridae from the lizardfish Saurida nebulosa Valenciennes, 1850 and Senga sp. from the eel Mastacembelus armatus (Lacepède, 1800), have been studied using transmission electron microscopy. Spermiogenesis included the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2215-1
更新日期：2011-07-01 00:00:00
abstract：:In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3526-9
更新日期：2013-10-01 00:00:00
abstract：:Filter-feeding molluscan shellfish can concentrate environmentally derived waterborne pathogens of humans, which can be utilized in the sanitary assessment of water quality. In the present study, oocysts of Cryptosporidium were detected in Bent mussels (Ischadium recurvum) at two Chesapeake Bay sites from which C. par...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050590
更新日期：1999-07-01 00:00:00
abstract：:There are first data on nematodes of Anas platyrhynchos Linnaeus, 1758 (mallard) and Bucephala clangula Linnaeus, 1758 (common goldeneye) from Northern Europe (Ladoga Lake region). The ducks were found to be infected with nine nematode species. A. platyrhynchos hosted eight nematode species and B. clangula was host to...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4697-3
更新日期：2015-10-01 00:00:00
abstract：:The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050519
更新日期：1999-02-01 00:00:00
abstract：:Chicken coccidiosis is a major parasitic disease caused by Eimeria spp. It is controlled and treated using chemical anticoccidial agents. Development of partial or complete resistance toward these anticoccidials is considered a major problem in poultry industry. Allicin is an organosulfur compound produced as a result...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4637-2
更新日期：2015-10-01 00:00:00
abstract：:The efficacy of abamectin 1%, when injected subcutaneously in cattle at a dose of 200 micrograms/kg body weight, against the larval stages (grubs) of the fly Dermatobia hominis was evaluated in two trials in endemic areas of Brazil and Argentina. Eighteen Holstein x Brahman castrated males and 16 Brahman-cross with na...
journal_title：Parasitology research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00931889
更新日期：1993-01-01 00:00:00
abstract：:Cyclospora cayetanensis was identified as the cause of large outbreaks of diarrhea in many parts of the world, but its host range and reservoirs remains poorly defined. Recently, oocysts resembling the C. cayetanensis were detected in dairy cattle fecal specimens from China. The 18S rDNA from two of these Cyclospora-l...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0380-z
更新日期：2007-04-01 00:00:00
abstract：:Interferon-tau (IFN-tau) treatment of Trichinella spiralis-infected BALB/c mice was investigated. The therapeutic regimen consisted of daily intraperitoneal injection of 10(4) U murine IFN-tau for 7 days, starting at 2 weeks post-infection. Striated muscle samples (diaphragm, thigh) were collected at 4, 8 and 12 weeks...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931641
更新日期：1991-01-01 00:00:00
abstract：:Ruminant coccidiosis is a gastrointestinal disease caused by parasites of the genus Eimeria. Environmental and climatic factors are relevant for the development, survival, and transmission of coccidiosis because Eimeria oocysts are able to survive in the environment for several weeks or months in favorable conditions ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06548-8
更新日期：2020-03-01 00:00:00
abstract：:Nitric oxide (NO) production by peritoneal macrophages was evaluated in Calomys callosus and Swiss mice during the course of infection with two strains of Trypanosoma cruzi. In C. callosus, no NO production was detected throughout the period of observation in animals infected with either parasite strain, except for a ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050336
更新日期：1997-01-01 00:00:00
abstract：:Pathogen-free rainbow trout (Oncorhynchus mykiss) aged 735 degree days were experimentally exposed to a low dose of infectious Myxobolus cerebralis (20 triactinomyxons fish(-1)). Three time periods were chosen for sampling that included 10 days (d), 67 d, and 5 months (mo) post exposure. Five diagnostic assays were us...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0166-3
更新日期：2006-09-01 00:00:00
abstract：:Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by C. meleagridis or C. baileyi with zoonotic potential have also been reported in domestic birds; and recent studies indicate the presence of new host-adapted species or genotype in birds. Therefore, accurately identifying isolates is...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1082-z
更新日期：2004-04-01 00:00:00
abstract：:DNA plasmid immunization is a novel approach of preventive and therapeutic vaccine. More than 100 DNA vaccines have been on preclinical or clinical phase trials, and four kinds of DNA vaccines for livestock have been approved by USDA, CFIA, and APVMA. Schistosomiasis is a worldwide parasitic disease, and vaccine immun...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3104-6
更新日期：2013-01-01 00:00:00
abstract：:Cryptosporidium spp. and Giardia duodenalis are protozoan parasites that cause diarrhea in humans and animals. Molecular data on Cryptosporidium spp. and G. duodenalis in calves in the Republic of Korea (ROK) is limited; thus, we investigated the prevalence of Cryptosporidium and Giardia in pre-weaned calves, analyzed...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06482-9
更新日期：2019-12-01 00:00:00
abstract：:Three juvenile nematode parasites were collected naturally from 90 (75 %) out of 120 specimens of the marine greater lizard fish Saurida undosquamis captured from water coasts at Hurghada City along the Red Sea in Egypt during the period from September 2013 to April 2014. Worms were identified on the basis of light an...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4285-y
更新日期：2015-03-01 00:00:00
abstract：:In latest years, the importance of the Melaleuca alternifolia essential oil (EO) has been greatly empathised due to its anti-microbial and anti-inflammatory effects, as well as to its toxic properties towards many arthropods of great medical and veterinary importance. In this research, the EO extracted from aerial par...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3651-5
更新日期：2014-01-01 00:00:00
abstract：:Ras are key components of diverse signal transduction pathways and play important roles in growth and development. To know about growth regulation in Clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cDNA library. The open reading frame contains 561 bp ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2147-9
更新日期：2011-04-01 00:00:00
