Abstract：
:In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding of D. repens was recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100% homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repens microfilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Bocková E,Rudolf I,Kočišová A,Betášová L,Venclíková K,Mendel J,Hubálek Zdoi
10.1007/s00436-013-3526-9subject
Has Abstractpub_date
2013-10-01 00:00:00pages
3465-70issue
10eissn
0932-0113issn
1432-1955journal_volume
112pub_type
杂志文章abstract：:Members of the Naegleria genus are free-living amoebae, and the only pathogenic specie described to date for humans is N. fowleri. However, as the complete genome of this specie has not been reported, non-pathogenic N. gruberi is employed to describe molecular pathways in N. fowleri. Regardless, certain mechanisms, su...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5293-x
更新日期：2017-01-01 00:00:00
abstract：:Dendrimers are repetitively branched molecules with a broad spectrum of applications, mainly for their antimicrobial properties and as nanocarriers for other molecules. Recently, our research group have synthesized and studied their activity against Acanthamoeba sp., causative agent of a severe ocular disease in human...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4205-1
更新日期：2015-02-01 00:00:00
abstract：:Ultrastructure of the trophozoite of Pneumocystis carinii was studied by the freeze-fracture technique. Nuclei and cytoplasmic organelles such as the endoplasmic reticulum, mitochondria, cytoplasmic vacuoles and small round bodies were observed. The mean number of nuclear pores was 8 per micron 2, which is small compa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00536469
更新日期：1987-01-01 00:00:00
abstract：:An arbitrary-primed RNA PCR differential display strategy was used to identify midgut genes of the reduviid bug Triatoma infestans that were differentially expressed after a blood meal. From interesting bands, 33 distinct cDNAs were cloned and sequenced. Although many had long open reading frames, most of the transcri...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0705-5
更新日期：2002-12-01 00:00:00
abstract：:Tachyzoites of Toxoplasma gondii attach to the macrophage surface and are internalized either by a phagocytic process, which can be inhibited by cytochalasin D, or by an active process, independent of host cell actin. Previous studies have shown that parasite attachment induces the secretion of macromolecules found in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008500
更新日期：2000-01-01 00:00:00
abstract：:A novel approach to control strategies for integrated blood-feeding parasite management is in high demand, including the use of biological control agents. The present study aims to determine the efficacy of optimized crude extract of actinomycetes strain LK1 as biological control agent against the fourth-instar larvae...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3585-y
更新日期：2013-11-01 00:00:00
abstract：:In three different areas in northern Germany, hippoboscids were collected from red deer (Cervus elaphus). Typanosomatids were demonstrated in the midgut and hindgut of 9/37 Lipoptena cervi. The trypanosomatids were morphologically similar to vector forms of the subgenus Megatrypanum that had been found in tabanids at ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00928691
更新日期：1991-01-01 00:00:00
abstract：:With the objective of identifying the helminths of Paroaria coronata, 40 birds were necropsied, and their organs and contents were examined. The parasites were preserved in 70 masculineGL alcohol and prepared for identification using standard techniques. The helminth fauna found in P. coronata were Aproctella carinii,...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1569-8
更新日期：2009-10-01 00:00:00
abstract：:Free-living amoebae (FLA) are opportunistic and ubiquitous protozoa that are widely found in various environmental sources. They are known to cause serious human infections. The aim of our study was to detect FLA and Acanthamoeba spp. in hospital water circuits. Eighty-four water samples were collected over a period o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4788-1
更新日期：2016-01-01 00:00:00
abstract：:Visceral larva migrans (VLM) syndrome caused by Toxocara canis larvae was first described in the 1950s. The role of other nematode larvae, i.e. the pig roundworm Ascaris suum as a causative agent of visceral larva migrans-associated symptoms like general malaise, cough, liver dysfunction, hypereosinophilia with hepato...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4196-y
更新日期：2015-01-01 00:00:00
abstract：:We have previously reported the involvement of cyclic AMP in regulating flagellar waveforms in Leishmania. Here, we investigated the roles of calcium, calmodulin, and calcineurin in flagellar motility regulation in L. donovani. Using high-speed videomicroscopy, we show that calcium-independent calmodulin and calcineur...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5608-6
更新日期：2017-11-01 00:00:00
abstract：:The nematode Teladorsagia circumcincta is a polymorphic species, morphs T. c. trifurcata and T. c. circumcincta being the most frequent. Among other traits, T. c. trifurcata has lower infectivity than T. c. circumcincta. Anthelmintic treatments that regularly remove populations should favour the more infective T. c. c...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100387
更新日期：2001-09-01 00:00:00
abstract：:A new neogregarine pathogen of the great spruce bark beetle, Dendroctonus micans (Coleoptera: Curculionidae, Scolytinae), is described based on light microscopy and ultrastructural characteristics. The pathogen infects the fat body and the hemolymph of the beetle. The infection was nonsynchronous so that different dev...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4497-9
更新日期：2015-08-01 00:00:00
abstract：:This work investigated the anti-amoebic activity of two samarium (Sm) complexes, the acyclic complex [bis(picrato)(pentaethylene glycol)samarium(III)] picrate-referred to as [Sm(Pic)2(EO5)](Pic)-and the cyclic complex [bis(picrato)(18-crown-6)samarium(III)] picrate-referred to as [Sm(Pic)2(18C6)](Pic). Both Sm complex...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5814-x
更新日期：2018-05-01 00:00:00
abstract：:The round goby Neogobius melanostomus is a bottom-dwelling fish native for the Ponto-Caspian basin, which started to colonize the Baltic Sea since 1991. The parasites of this fish species in the southwestern Baltic region are studied for the first time. The round goby in the SW Baltic Sea was infected with 12 parasite...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2321-8
更新日期：2011-09-01 00:00:00
abstract：:In order to assess the potential of the stem bark of Kigelia africana (Lam.) Benth as source of new anti-malarial leads, n-hexane and ethyl acetate (EtOAc) extracts and four compounds isolated from the stem bark were screened in vitro against the chloroquine-resistant W-2 and two field isolates of Plasmodium falciparu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2363-y
更新日期：2011-06-01 00:00:00
abstract：:Trypanosoma cruzi causes Chagas' disease, a systemic infection that affects cells of meso-, endo-, and ectodermic origin. However, as far as we know, the presence of T. cruzi stages in bone has not been reported previously, and it has scarcely been investigated in cartilage. We inoculated 7- and 20-day-old (8 and 15 g...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0211-2
更新日期：2006-11-01 00:00:00
abstract：:Paramyosin is a muscle protein that probably plays a role in the survival of the larval stage of Taenia solium during its prolonged host-parasite relationship. Here we describe the structure of the gene coding for the paramyosin of T. solium. The characterization of two clones obtained from a genomic library showed th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0761-x
更新日期：2003-03-01 00:00:00
abstract：:Mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. Dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. Recently, transmission has strongly increased in urban and semiurban areas, becoming a major international public ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4339-9
更新日期：2015-04-01 00:00:00
abstract：:Human cystic and alveolar echinococcoses are zoonotic diseases caused by the larval stages of Echinococcus granulosus and Echinococcus multilocularis, respectively. As the diseases are co-endemic in many areas of the world, a simple and rapid test for the differential diagnosis of cystic echinococcosis (CE) and alveol...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3550-9
更新日期：2013-10-01 00:00:00
abstract：:Trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral parasitic sexually transmitted infection in the world. Presently, there are no reports on comparative sequence analysis as well as on the identification of phylogenetic positions of T. vaginalis isolates from the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1635-2
更新日期：2009-12-01 00:00:00
abstract：:The development of the metacestode stages of Arostrilepis microtis (Eucestoda: Hymenolepididae) studied on the basis of an experimental infection of springtails (Collembola: Entomobryidae) is described for the first time. A complete description of the ontogeny from oncosphere to fully a developed metacestode is given....
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5929-0
更新日期：2018-08-01 00:00:00
abstract：:Among tick species, members of the subfamily Amblyomminae have received special attention, since they serve as vectors for pathogens such as Rickettsia spp. and display cryptic species complexes that make their taxonomical classification challenging. Amblyomma ovale, Amblyomma maculatum, and other species of the genus...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5592-x
更新日期：2017-11-01 00:00:00
abstract：:This study describes the ultrastructural organisation of the spermatozoon of a digenean Enodiotrema reductum (Pligiorchiida: Plagiorchiidae) from the green turtle Chelonia mydas (Linnaeus, 1758). This is the first report of E. reductum from Senegal. The mature spermatozoon of E. reductum is filiform and exhibits two a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2911-0
更新日期：2012-08-01 00:00:00
abstract：:The effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro application was done...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5056-8
更新日期：2016-08-01 00:00:00
abstract：:Catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including Schistosoma japonicum. S. japonicum tyrosine hydroxylase (SjTH) was amplified by rapid amplification of cDNA ends polymerase chain reaction that shows strong homo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2347-y
更新日期：2011-10-01 00:00:00
abstract：:The morphology and ecology are described for four sibling species of digenean metacercariae: Meiogymnophallus minutus (Cobbold 1859), M. strigatus (Lebour 1908) from the estuarine cockle Cerastoderma edule L. and M. fossarum (Bartoli, 1965) and M. rebecqui (Bartoli, 1983) comb. nov. from the brackish water lagoon cock...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050153
更新日期：1996-01-01 00:00:00
abstract：:Different Tunga penetrans isolates from various hosts obtained from South America (Fortaleza. Brazil) have been studied by nucleotide sequence comparison of the first and the second internal transcribed spacer (ITS1, ITS2) of the ribosomal deoxyribonucleic acid (rDNA) and part of the mitochondrial 16S rDNA. Results sh...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0743-0
更新日期：2008-01-01 00:00:00
abstract：:This work describes a simple method to yield large amounts of Leishmania amastigote-like forms in axenic cultures using promastigotes as the starting population. The method described induced extracellular amastigote transformation of Leishmania amazonensis (97%), Leishmania braziliensis (98%) and Leishmania chagasi (9...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0695-3
更新日期：2002-11-01 00:00:00
abstract：:An easy and rapid tool for the individual identification of Haemonchus species in domestic ruminants might be very useful in the study of natural populations, especially when two (or three) species are sympatric. Techniques based on cytology, cuticular-ridge patterns, sublateral hypodermic chords, or molecular biology...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050213
更新日期：1997-01-01 00:00:00
