Abstract：
:In order to assess the potential of the stem bark of Kigelia africana (Lam.) Benth as source of new anti-malarial leads, n-hexane and ethyl acetate (EtOAc) extracts and four compounds isolated from the stem bark were screened in vitro against the chloroquine-resistant W-2 and two field isolates of Plasmodium falciparum using lactate dehydrogenase assay. The products were also tested for their cytotoxicity on LLC/MK2 monkey kidney cells. The EtOAc extract exhibited a significant antiplasmodial activity (IC(50) = 11.15 μg/mL on W-2; 3.91 and 4.74 μg/mL on field CAM10 and SHF4 isolates, respectively), whereas the n-hexane fraction showed a weak activity (IC(50) = 73.78 μg/mL on W-2 and 21.85 μg/mL on SHF4). Three out of the four compounds showed good activity against all the three different parasite strains (IC(50) <5 μM). Specicoside exhibited the highest activity on W-2 (IC(50) = 1.54 μM) followed by 2β, 3β, 19α-trihydroxy-urs-12-en-28-oic acid (IC(50) = 1.60 μM) and atranorin (IC(50) = 4.41 μM), while p-hydroxycinnamic acid was the least active (IC(50) =53.84 μM). The EtOAc extract and its isolated compounds (specicoside and p-hydroxycinnamic acid) were non-cytotoxic (CC(50) > 30 μg/mL), whereas the n-hexane extract and two of its products, atranorin and 2β, 3β, 19α-trihydroxy-urs-12-en-28-oic acid showed cytotoxicity at high concentrations, with the last one being the most toxic (CC(50) = 9.37 μg/mL). These findings justify the use of K. africana stem bark as antimalaria by traditional healers of Western Cameroon, and could constitute a good basis for further studies towards development of new leads or natural drugs for malaria.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Zofou D,Kengne AB,Tene M,Ngemenya MN,Tane P,Titanji VPdoi
10.1007/s00436-011-2363-ysubject
Has Abstractpub_date
2011-06-01 00:00:00pages
1383-90issue
6eissn
0932-0113issn
1432-1955journal_volume
108pub_type
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,多中心研究
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pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-020-06663-x
更新日期：2020-07-01 00:00:00
