Occurrence and molecular characterization of Giardia duodenalis in lambs in Djelfa, the central steppe of Algeria.

Abstract:

:Little is known of the prevalence and genetic identity of Giardia duodenalis in sheep in Algeria. The present study aimed at characterizing G. duodenalis in lambs up to 6 months of age in Djelfa, Algeria. A total of 346 fecal specimens were collected from 28 farms and screened for G. duodenalis cysts by zinc sulfate flotation microscopy, and positive specimens were confirmed using a direct immunofluorescence assay. Microscopy-positive specimens were analyzed by PCR and sequence analysis of the triosephosphate isomerase and glutamate dehydrogenase genes to determine G. duodenalis assemblages. Coprological examination indicated that the overall infection rate was 7.0% (24/346). Lambs under 3 months of age had higher infection rate (18/197, 9.0%) than older (6/149, 4.0%) animals, and animals with diarrhea (7/44, 16.0%) had higher infection rate than animals without diarrhea (17/302, 5.6%). PCR sequence analyses of the 15 G. duodenalis isolates revealed the presence of assemblages A in 6 isolates, assemblage E in 7 isolates, and both in 2 isolates. Assemblage A was only found in pre-weaned lambs with diarrhea, while assemblage E was mostly found in post-weaned lambs without diarrhea. The assemblage E isolates from sheep were genetically related to those from cattle in Algeria, while assemblage A isolates were from a well-known subtype prevalent in humans. Data generated from the study improve our understanding of the transmission of G. duodenalis in Algeria.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Benhassine S,Baroudi D,Hakem A,Thomas M,Laatamna A,Belkessa S,Feng Y,Roellig D,Chen X,Adjou KT,Xiao L

doi

10.1007/s00436-020-06808-y

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

2965-2973

issue

9

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-020-06808-y

journal_volume

119

pub_type

杂志文章
  • Metabolic transition in the development of Hymenolepis diminuta (Cestoda).

    abstract::Cysticercoids as well as 6-, 10-, and 14-day Hymenolepis diminuta were evaluated in terms of enzymatic activities related to phosphoenolpyruvate (PEP) utilization and mitochondrial succinate accumulation. The data obtained support a transition toward anaerobic electron-transport-dependent succinate accumulation, chara...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050487

    authors: Fioravanti CF,Walker DJ,Sandhu PS

    更新日期:1998-10-01 00:00:00

  • Seasonal variation of strongylosis in working donkeys of Ethiopia: a cross-sectional and longitudinal studies.

    abstract::Helminths are one of the major health problems of working donkeys, often with heavy worm burden and contributing to their early demise and/or reduction in their work output. Cross-sectional and longitudinal studies were conducted to investigate the current infection prevalence and level of strongyles infection donkeys...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5485-z

    authors: Dibaba MD,Getachew AM,Assefa Z,Fanta A,Etana M,Firew S,Goshu L,Burden F

    更新日期:2017-07-01 00:00:00

  • Tortoise tick Hyalomma aegyptium as long term carrier of Q fever agent Coxiella burnetii--evidence from experimental infection.

    abstract::The experimental study investigated the ability of tortoise tick Hyalomma aegyptium to play a role in forming and maintaining natural foci of Q fever. We tested the competence of H. aegyptium larvae to acquire Coxiella burnetii infection from mammals, serve as a C. burnetii vector between mammalian hosts, and be a lon...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-2037-1

    authors: Siroký P,Kubelová M,Modrý D,Erhart J,Literák I,Spitalská E,Kocianová E

    更新日期:2010-11-01 00:00:00

  • Effects of sex and age on the susceptibility of C57BL/6J mice to infection with Brachylaima cribbi and the course of infection in NOD SCID mice.

    abstract::The C57BL/6J strain of Mus musculus is susceptible to the terrestrial trematode Brachylaima cribbi. The duration of infection in these mice is generally 9-12 weeks with a peak excretion of eggs at 4 weeks post-infection (wpi). The effects of age and sex on the course of infection were investigated by comparing infecti...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-002-0642-3

    authors: Butcher AR,Palethorpe HM,Grove DI

    更新日期:2002-07-01 00:00:00

  • Role of surface components in the process of interaction of Giardia duodenalis with epithelial cells in vitro.

    abstract::Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF02351874

    authors: Pegado MG,de Souza W

    更新日期:1994-01-01 00:00:00

  • Occurrence of Leishmania infantum and associated histological alterations in the genital tract and mammary glands of naturally infected dogs.

    abstract::The objectives of this study were to evaluate the occurrence of Leishmania infantum in the male and female genital tract and female mammary glands of dogs and the parasite burden and to identify histological alterations associated with this protozoan. Twenty male and 20 female Leishmania-seropositive dogs with isolati...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-4987-4

    authors: Boechat VC,Mendes Junior AA,Madeira Mde F,Ferreira LC,Figueiredo FB,Rodrigues Fd,Oliveira Vda C,de Oliveira Rde V,Menezes RC

    更新日期:2016-06-01 00:00:00

  • Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens.

    abstract::In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-5049-7

    authors: Mokhtarian K,Akhlaghi L,Meamar AR,Razmjou E,Manouchehri Naeini K,Gholami S,Najafi Samei M,Falak R

    更新日期:2016-08-01 00:00:00

  • Coccidia (Apicomplexa: Eimeriidae) of elk (Alces alces) in Poland.

    abstract::Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-012-3262-6

    authors: Pyziel AM,Demiaszkiewicz AW

    更新日期:2013-05-01 00:00:00

  • Factors influencing spatial variation and abundance of a mermithid parasite in sand hoppers.

    abstract::The impact of parasites on host population dynamics depends on local abundance of the parasites, which may vary considerably across spatial scales. In sand hopper populations, mermithid parasites have major impacts on host dynamics, which may vary among spatially separated populations due to the sand hopper's wide, pa...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-4254-5

    authors: Rasmussen TK,Randhawa HS

    更新日期:2015-03-01 00:00:00

  • Antimalarial potential of leaves of Chenopodium ambrosioides L.

    abstract::In an effort to identify novel therapeutic alternatives for the treatment of malaria, the present study evaluated the antimalarial effect of the crude hydroalcoholic extract (HCE) from the leaves of Chenopodium ambrosioides L. For this purpose, the molecular affinity between the total proteins from erythrocytes infect...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-5216-x

    authors: Cysne DN,Fortes TS,Reis AS,de Paulo Ribeiro B,Dos Santos Ferreira A,do Amaral FM,Guerra RN,Marinho CR,Nicolete R,Nascimento FR

    更新日期:2016-11-01 00:00:00

  • Fine structure and cytochemical analysis of the intestinal wall along the body of adult female of Litomosoides chagasfilhoi (Nematoda: Filarioidea).

    abstract::Litomosoides chagasfilhoi is a filariid nematode parasite of the abdominal cavity of the wild rodent Akodon cursor (Winge, 1887), that has been described and used in Brazil as a new model for human filariasis. The fine structure of the intestine of this nematode was analyzed based on observations made by light and tra...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-0092-9

    authors: de Moraes Neto AH,Cunha GS,Ferreira TF,de Carvalho SN,Guimarães EV,de Souza W

    更新日期:2006-05-01 00:00:00

  • Toxoplasma gondii seroprevalence in beef cattle raised in Italy: a multicenter study.

    abstract::Toxoplasmosis represents an important public health issue, with the consumption of raw or undercooked meat being a major way of human infection. The role of beef in the transmission of the parasite to humans is questioned due to lower quantity of tissue cysts compared with other meat-producing species. However, the ha...

    journal_title:Parasitology research

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s00436-020-06878-y

    authors: Gazzonis AL,Marino AMF,Garippa G,Rossi L,Mignone W,Dini V,Giunta RP,Luini M,Villa L,Zanzani SA,Manfredi MT

    更新日期:2020-11-01 00:00:00

  • Correction to: Acanthamoeba of three morphological groups and distinct genotypes exhibit variable and weakly inter-related physiological properties.

    abstract::The authors recognized a mistake in Abstract and in the Discussion section. ...

    journal_title:Parasitology research

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s00436-018-5884-9

    authors: Possamai CO,Loss AC,Costa AO,Falqueto A,Furst C

    更新日期:2018-06-01 00:00:00

  • Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China.

    abstract::This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat s...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0851-x

    authors: Wang R,Zhang L,Ning C,Feng Y,Jian F,Xiao L,Lu B,Ai W,Dong H

    更新日期:2008-04-01 00:00:00

  • Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites.

    abstract::Allicin is an active ingredient of garlic that has antibacterial, antifungal, antiviral, and antiprotozoal activity. However, the inhibitory effects of allicin on Babesia parasites have not yet been examined. In the present study, allicin was tested as a potent inhibitor against the in vitro growth of bovine and equin...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3654-2

    authors: Salama AA,AbouLaila M,Terkawi MA,Mousa A,El-Sify A,Allaam M,Zaghawa A,Yokoyama N,Igarashi I

    更新日期:2014-01-01 00:00:00

  • The sources and metabolic dynamics of Schistosoma japonicum DNA in serum of the host.

    abstract::The polymerase chain reaction (PCR) assay has turned out to be one of the most potential tools for diagnosis of schistosomiasis. However, the source and metabolic dynamics of Schistosoma japonicum DNA in the blood of hosts is not clear. In this study, rabbit models with monosexual and mixed sexual cercariae infection ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-012-3115-3

    authors: Xu J,Liu AP,Guo JJ,Wang B,Qiu SJ,Sun H,Guan W,Zhu XQ,Xia CM,Wu ZD

    更新日期:2013-01-01 00:00:00

  • Prevalence of helminths in horses in the state of Brandenburg, Germany.

    abstract::The objective of the study was to estimate the prevalence of helminths in the horse population of the state of Brandenburg, Germany. One hundred and twenty-six horse farms in the state were selected by randomised stratified sampling. In total, 1,407 horses across all farms were examined coproscopically. The experiment...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2362-z

    authors: Hinney B,Wirtherle NC,Kyule M,Miethe N,Zessin KH,Clausen PH

    更新日期:2011-05-01 00:00:00

  • New insights into the life cycle of Platynosomum (Trematoda: Dicrocoeliidae).

    abstract::The platynosomiasis, a worldwide parasitic disease with importance for domestic cat, has an etiological agent species of trematodes of the genus Platynosomum, whose complete life cycles are not yet known. The real role of lizards in the transmission of this dicrocoeliid parasite (as obligatory intermediate or parateni...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-3926-5

    authors: Pinto HA,Mati VL,de Melo AL

    更新日期:2014-07-01 00:00:00

  • Disaccharidase deficiencies in Mongolian gerbils (Meriones unguiculatus) protected against Giardia lamblia.

    abstract::The activities of the disaccharidases lactase, maltase, sucrase and trehalase were examined in gerbils during Giardia lamblia infections. In a primary infection with trophozoites, the activities of all four enzymes were reduced from day 10 post-infection (p.i.) and remained at low levels well past the elimination phas...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00932025

    authors: Mohammed SR,Faubert GM

    更新日期:1995-01-01 00:00:00

  • The efficacy of inhibitors involved in spermidine metabolism in Plasmodium falciparum, Anopheles stephensi and Trypanosoma evansi.

    abstract::In the present study, we have tested the effect of different polyamine inhibitors of the spermidine metabolizing enzymes deoxyhypusine synthase and homospermidine synthase in different chloroquine resistant Plasmodium falciparum strains, in the mosquito Anopheles stephensi (Diptera: Culicidae) and in a Trypanosoma eva...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-004-1162-0

    authors: Moritz E,Seidensticker S,Gottwald A,Maier W,Hoerauf A,Njuguna JT,Kaiser A

    更新日期:2004-09-01 00:00:00

  • Impact of trematode infections on periphyton grazing rates of freshwater snails.

    abstract::In freshwater ecosystems, snails can significantly influence the competition between primary producers through grazing of periphyton. This activity can potentially be modified by trematodes, a large group of parasites which mostly use molluscs as the first intermediate host. Available studies, however, show contradict...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-018-6052-y

    authors: Vivas Muñoz JC,Hilt S,Horák P,Knopf K

    更新日期:2018-11-01 00:00:00

  • The putative cyclooctadepsipeptide receptor depsiphilin of the canine hookworm Ancylostoma caninum.

    abstract::The G-Protein-coupled receptor Hc110-R of Haemonchus contortus and its orthologue in Caenorhabditis elegans, the latrophilin-like protein 1 (LAT-1), were shown to play a role in the mode of action of the new anthelmintic compound emodepside. C. elegans LAT-1 knockout mutants showed a decreased paralysing effect of emo...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-009-1500-3

    authors: Krüger N,Harder A,von Samson-Himmelstjerna G

    更新日期:2009-08-01 00:00:00

  • Molecular evidence of intraspecific variability in different habitat-related populations of Triatoma dimidiata (Hemiptera: Reduviidae) from Costa Rica.

    abstract::Intraspecific genetic variation among Triatoma dimidiata (Hemiptera: Reduviidae) from seven Costa Rican populations and from different domestic, peridomestic, and sylvatic ecotopes were analyzed. The complete nucleotide sequence of the nuclear ribosomal DNA internal transcribed spacer (ITS-2) and partial sequences of ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-1762-9

    authors: Blandón-Naranjo M,Zuriaga MA,Azofeifa G,Zeledón R,Bargues MD

    更新日期:2010-03-01 00:00:00

  • In vitro leucocyte proliferative responses and lymphocyte sub-types in guinea pigs with genetically determined high- and low-level responsiveness to Trichostrongylus colubriformis.

    abstract::In vitro leucocyte proliferative responses to parasite antigens and to mitogens as well as lymphocyte sub-types were compared in guinea pigs with genetically determined differences in their ability to express protective immunity against Trichostronylus colubriformis infection. Proliferative responses to parasite antig...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050048

    authors: Manjili MH,Sangster NC,Rothwell TL

    更新日期:2000-04-01 00:00:00

  • The colonization of the invasive round goby Neogobius melanostomus by parasites in new localities in the southwestern Baltic Sea.

    abstract::The round goby Neogobius melanostomus is a bottom-dwelling fish native for the Ponto-Caspian basin, which started to colonize the Baltic Sea since 1991. The parasites of this fish species in the southwestern Baltic region are studied for the first time. The round goby in the SW Baltic Sea was infected with 12 parasite...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2321-8

    authors: Kvach Y,Winkler HM

    更新日期:2011-09-01 00:00:00

  • Outbreak of toxoplasmosis in a flock of domestic chickens (Gallus gallus domesticus) and guinea fowl (Numida meleagris).

    abstract::Toxoplasmosis is a disease with a worldwide distribution that affects a wide variety of animal species, though with rare descriptions in chickens. We describe the clinical, epidemiological, pathological, and molecular aspects of a toxoplasmosis outbreak in domestic chickens and guinea fowl in southern Brazil. The floc...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-019-06233-w

    authors: Vielmo A,Pena HFJ,Panziera W,Bianchi RM,De Lorenzo C,Oliveira S,Alves BF,Gennari SM,Pavarini SP,de Barros CSL,Driemeier D

    更新日期:2019-03-01 00:00:00

  • The type and mysorensis forms of the Anopheles stephensi (Diptera: Culicidae) in India exhibit identical ribosomal DNA ITS2 and domain-3 sequences.

    abstract::Anopheles (Cellia) stephensi Liston 1901 is one of the major malaria vectors in the Indian subcontinent, Iran, and the Middle East. Three races in this species, namely A. stephensi stephensi (type form), A. stephensi variety mysorensis, and A. stephensi intermediate form, have earlier been reported by several investig...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-0930-7

    authors: Alam MT,Bora H,Das MK,Sharma YD

    更新日期:2008-06-01 00:00:00

  • The field efficacy of garlic extract against Dermanyssus gallinae in layer farms of Babol, Iran.

    abstract::Dermanyssus gallinae (Acari: Mesostigmata) is the most important hematophagous ectoparasite in layer farms in many countries. The reproduction rate of the parasite is rapid and can be completed in a week under favorable conditions. The parasite has direct and indirect effects on birds. It can also act as a vector for ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-3759-2

    authors: Faghihzadeh Gorji S,Faghihzadeh Gorji S,Rajabloo M

    更新日期:2014-03-01 00:00:00

  • The Gyrodactylus (Monogenea, Gyrodactylidae) parasite fauna of freshwater sand gobies (Teleostei, Gobioidei) in their centre of endemism, with description of seven new species.

    abstract::While Gobioidei comprises showcases of (adaptive) radiation, the scientific interest they yielded did not ensure full understanding of goby biodiversity. Even in a well-studied region like Europe, wide knowledge gaps remain. Sand gobies represent one of the few clades whose monogenean parasites have been thoroughly st...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3693-8

    authors: Vanhove MP,Economou AN,Zogaris S,Giakoumi S,Zanella D,Volckaert FA,Huyse T

    更新日期:2014-02-01 00:00:00

  • Trematode maturation patterns in a migratory snail host: What happens during upshore residency in a Mediterranean lagoon?

    abstract::Maturation of trematode larval stages is expected to be temporally and spatially adapted to maximise the encounter with the adequate downstream host, i.e. the host, which will be infected by this parasite stage. Since studies on intramolluscan parasite maturation are scarce but important in the context of parasite tra...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4774-7

    authors: Born-Torrijos A,Raga JA,Holzer AS

    更新日期:2016-02-01 00:00:00