Characterization of development-related genes for the cestode Bothriocephalus acheilognathi.

Abstract:

:Differential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were upregulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and the three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Luo HL,Nie P,Chang MX,Song Y,Yao WJ

doi

10.1007/s00436-004-1205-6

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

265-274

issue

4

eissn

0932-0113

issn

1432-1955

journal_volume

94

pub_type

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