Abstract:
:Differential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were upregulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and the three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Luo HL,Nie P,Chang MX,Song Y,Yao WJdoi
10.1007/s00436-004-1205-6subject
Has Abstractpub_date
2004-10-01 00:00:00pages
265-274issue
4eissn
0932-0113issn
1432-1955journal_volume
94pub_type
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