Abstract：
:Haemonchus contortus isolates were evaluated for benzimidazole (BZ) resistance or susceptibility by allele-specific PCR based on β-tubulin isotype 1 gene polymorphisms at the F167Y, E198A, and F200Y sites. Two isolates, one presumed susceptible from wild pronghorn antelope (PH) and one known to be resistant from goats (VM), were also assayed phenotypically for BZ resistance or susceptibility in the larval development assay (Drenchrite®). The BZ EC50 was 0.198 μM (intermediate between susceptible and weak resistant) for PH with critical well 5 (intermediate between susceptible and weak resistant) and 1.456 μM (intermediate weak resistant and resistant) for VM with critical well 8.5 (resistant). Genotypically, DNA extracted from pooled VM L3 larvae in the Drenchrite® wells with the highest BZ concentration was homozygous susceptible (SS) at the F167Y and E198A sites and homozygous resistant (RR) at the F200Y site by PCR, and sequence analysis bore this out. PH L3 larvae DNA from a control well (no BZ) was SS at all three sites by PCR, confirmed by sequence analysis. All single adult worm samples (N = 21) from PH, VM, Egypt goat (EG), and a Texas llama were SS at F167Y and E198A by PCR; however, only 3 PH worms and 1 EG worm were SS at F200Y. Three additional PH worms were RS and upon cloning two clones were identified as resistant by sequencing and two as susceptible. Clones from single adult worms VM, llama, and EG samples that were RR by PCR at F200Y were sequence verified as resistant. In this study, F200Y was the most frequently found genotypic marker for BZ resistance or susceptibility in the different Haemonchus isolates.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Arafa WM,Holman PJ,Craig TMdoi
10.1007/s00436-016-5357-ysubject
Has Abstractpub_date
2017-02-01 00:00:00pages
797-807issue
2eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5357-yjournal_volume
116pub_type
杂志文章abstract：:Acanthamoeba are found in the environment, particularly in water, all over the world. The genus is currently classified into 20 different genotypes, T1-T20. In this study, 63 natural water samples from 11 provinces in northeast Thailand were collected and cultured on non-nutrient agar plates. Positive samples by cultu...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1436-1
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0558-z
更新日期：2007-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4417-z
更新日期：2015-06-01 00:00:00
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journal_title：Parasitology research
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doi：10.1007/s00436-008-0930-7
更新日期：2008-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0382-x
更新日期：2007-04-01 00:00:00
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更新日期：2004-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00933778
更新日期：1994-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2501-6
更新日期：2012-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5814-x
更新日期：2018-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050359
更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2011-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2009-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5135-x
更新日期：2016-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-11-01 00:00:00
abstract：:Ticks can significantly affect the health and fitness of the host. Seasonal population dynamics of ticks play a vital role in disease transmission and the shaping of life-history traits of both tick and host. In this study, we examine the seasonal population dynamics of Ixodes hirsti in South Australia. For 2 years, w...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-09-01 00:00:00
abstract：:Giardia is a prevalent intestinal parasitic infection. The trophozoite structural protein a1-giardin (a1-g) and the cyst protein cyst wall protein 2 (CWP2) have shown promise as Giardia vaccine antigen candidates in murine models. The present study assesses the genetic diversity of a1-g and CWP2 between and within ass...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5516-9
更新日期：2017-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06854-6
更新日期：2020-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5810-1
更新日期：2018-05-01 00:00:00
abstract：:Multiple schistosome and soil-transmitted nematode infections are frequently reported in human populations living in tropical areas of developing countries. In addition to exposure factors, the host immune response plays an important role in helminth control and morbidity in hosts with multiple infections; however, th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4706-6
更新日期：2015-12-01 00:00:00
abstract：:Sarcocystis sarcocysts are common in muscles of herbivores but are rare in muscles of carnivores. Here, we report sarcocysts in the muscles of a gray wolf (Canis lupus) from Alaska, USA, for the first time. Sarcocysts extracted from the tongue of the wolf were up to 900 μm long and slender and appeared to have a relat...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5067-5
更新日期：2016-07-01 00:00:00
