Abstract：
:This work investigated the anti-amoebic activity of two samarium (Sm) complexes, the acyclic complex [bis(picrato)(pentaethylene glycol)samarium(III)] picrate-referred to as [Sm(Pic)2(EO5)](Pic)-and the cyclic complex [bis(picrato)(18-crown-6)samarium(III)] picrate-referred to as [Sm(Pic)2(18C6)](Pic). Both Sm complexes caused morphological transformation of the protozoa Acanthamoeba from its native trophozoite form carrying a spine-like structure called acanthopodia, to round-shaped cells with loss of the acanthopodia structure, a trademark response to environmental stress. Further investigation, however, revealed that the two forms of the Sm complexes exerted unique cytotoxicity characteristics. Firstly, the IC50 of the acyclic complex (0.7 μg/mL) was ~ 10-fold lower than IC50 of the cyclic Sm complex (6.5 μg/mL). Secondly, treatment of the Acanthamoeba with the acyclic complex caused apoptosis of the treated cells, while the treatment with the cyclic complex caused necrosis evident by the leakage of the cell membrane. Both treatments induced DNA damage in Acanthamoeba. Finally, a molecular docking simulation revealed the potential capability of the acyclic complex to form hydrogen bonds with profilin-a membrane protein present in eukaryotes, including Acanthamoeba, that plays important roles in the formation and degradation of actin cytoskeleton. Not found for the cyclic complex, such potential interactions could be the underlying reason, at least in part, for the much higher cytotoxicity of the acyclic complex and also possibly, for the observed differences in the cytotoxicity traits. Nonetheless, with IC50 values of < 10 μg/mL, both the acyclic and cyclic Sm complexes feature a promising potential as cytotoxic agents to fight amoebic infections.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Kusrini E,Hashim F,Gunawan C,Mann R,Azmi WNNWN,Amin NMdoi
10.1007/s00436-018-5814-xsubject
Has Abstractpub_date
2018-05-01 00:00:00pages
1409-1417issue
5eissn
0932-0113issn
1432-1955pii
10.1007/s00436-018-5814-xjournal_volume
117pub_type
杂志文章abstract：:An endopeptidase specific to the Plasmodium falciparum erythrocytic schizont stage and to free merozoites was detected using the fluorogenic GlcA-Val-Leu-Gly-Lys(or Arg)-AEC substrate. The enzyme was purified by high performance liquid chromatography (HPLC); its optimal activity was around pH 7.5 and its isoelectric p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930972
更新日期：1989-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1821-2
更新日期：2010-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2009-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3181-6
更新日期：2013-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0725-2
更新日期：2007-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1810-5
更新日期：2010-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00935425
更新日期：1991-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3150-0
更新日期：2013-01-01 00:00:00
abstract：:Trypanosoma cruzi and Trypanosoma rangeli are kinetoplastid parasites which are able to infect humans in Central and South America. Misdiagnosis between these trypanosomes can be avoided by targeting barcoding sequences or genes of each organism. This work aims to analyze the feasibility of using species-specific mark...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3872-2
更新日期：2014-06-01 00:00:00
abstract：:Mosquitoes (Diptera: Culicidae) are a key threat for millions of people worldwide, since they act as vectors for devastating pathogens and parasites. The standard method of utilisation of morphological characters becomes challenging due to various factors such as phenotypical variations. We explored the complementary ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4726-2
更新日期：2016-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00934382
更新日期：1991-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5664-y
更新日期：2018-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3674-y
更新日期：2014-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4196-y
更新日期：2015-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s004360100386
更新日期：2001-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4788-1
更新日期：2016-01-01 00:00:00
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更新日期：2013-04-01 00:00:00
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更新日期：2015-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1835-9
更新日期：2010-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2002-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050072
更新日期：1996-01-01 00:00:00
abstract：:Aelurostrongylus abstrusus (Nematoda, Metastrongyloidea) causes verminous pneumonia in cats worldwide. This study evaluated the seroprevalence of A. abstrusus antibodies in 220 stray and free-roaming cats from insular (Mykonos, Crete, Skopelos) and continental (Thessaloniki, Attica) Greece. The results were compared w...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06839-5
更新日期：2020-09-01 00:00:00
abstract：:The dynamics of production and excretion of leukotrienes by parasitic larvae of Oesophagostomum dentatum and their role for the development of the larvae were studied. Larvae were cultured in vitro to the fourth stage (L4). Leukotriene B4 (LTB4) was detected in homogenates and in supernatant of larvae, with the homoge...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1671-y
更新日期：2010-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100490
更新日期：2001-12-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932628
更新日期：1994-01-01 00:00:00
abstract：:Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lys...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931595
更新日期：1993-01-01 00:00:00
