Dose- and time-dependent functional and structural damage to the colon mucosa by Entamoeba histolytica trophozoite lysates.

Abstract:

:Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lysates of the E. histolytica HM1 virulent strain. Exposure to lysates for up to 90 min produced dose- and time-dependent effects on the colon, consisting of (a) increased decay rates for potential difference, short-circuit current, and transmural resistance and (b) mucosal damage ranging from vacuolation at the bases and shortening of epithelial cells to the loss of intercellular junctions, destruction of microvilli, and necrosis of interglandular epithelial zones. This acute model of intestinal amebiasis is sensitive, fast, and reliable.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Navarro-García F,López-Revilla R,Tstusumi V

doi

10.1007/BF00931595

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

517-22

issue

6

eissn

0932-0113

issn

1432-1955

journal_volume

79

pub_type

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