Abstract:
:The distribution of actin filaments in the spermatogenic cells of Fasciola hepatica was determined using a fluorescent derivative of phalloidin. Actin was localised primarily in the region of separation of a secondary spermatogonium from a primary spermatogonium, in the inner faces at the centre of four-cell clusters of tertiary spermatogonia and in the cytophore region of spermatocyte and spermatid rosettes. The effect of the microfilament inhibitor cytochalasin B (100 micrograms/ml) on the ultrastructure of the spermatogenic cells was determined in vitro by transmission electron microscopy using tissue-slice material. Cytochalasin B treatment led to the formation of bi- and multinucleate cells, whose frequency increased with progressively longer incubation periods. Few typical rosettes of spermatocyte and spermatid cells were evident from 6 h onwards, being replaced by syncytial masses of cells. Spermatozoon formation became abnormal in the longer treatment periods, the spermatozoa containing variable numbers of axonemes and an altered distribution of cortical microtubules. Multiple axonemes were observed in the cytoplasm of spermatid cells. The results are discussed in relation to the established role of actin in the cytokinesis phase of cell division and to the effects of cytochalasin B on other tissues and organ systems within the fluke.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Stitt AW,Fairweather I,Johnston CFdoi
10.1007/BF00935425subject
Has Abstractpub_date
1991-01-01 00:00:00pages
123-8issue
2eissn
0932-0113issn
1432-1955journal_volume
77pub_type
杂志文章abstract::Saprophytic soil fungi can exert ovicidal and ovistatic effects on helminths with differing degrees of efficiency. The representatives of such fungi from temperate regions, Paecilomyces lilacinus (Thom) Samson and P. marquandii (Masse) Hughes, exhibit recognized ovicidal activity on some nematodes. We evaluated the ac...
journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
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journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932413
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journal_title:Parasitology research
pub_type: 杂志文章
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journal_title:Parasitology research
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