Abstract：
:High resolution two-dimensional separation of Toxoplasma gondii tachyzoite lysate revealed up to 224 distinct protein spots in Coomassie-stained gel. Computional matching of 14 digitized gels yielded a standard two-dimensional proteome map. The excretory T. gondii dense granule proteins GRA1-GRA8, S16/acid phosphatase, nucleoside triphosphate hydrolase, and H4 were identified by Western blotting of both total gel and isolated protein spots. In addition, two excretory antigens defined by parasite-specific monoclonal T cells, p36 and p40, were mapped by a novel T-cell blotting technique based on electroeluting single protein spots and testing the eluates for antigenic activity against the T-cell clones. In summary, these results represent a first step in Toxoplasma proteome analysis.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Dlugonska H,Dytnerska K,Reichmann G,Stachelhaus S,Fischer HGdoi
10.1007/s004360000367subject
Has Abstractpub_date
2001-08-01 00:00:00pages
634-7issue
8eissn
0932-0113issn
1432-1955journal_volume
87pub_type
杂志文章abstract：:In the present study, the morphology and morphometric characterization of Thelohanellus niloticus sp. nov., a new myxozoan belonging to genus Thelohanellus Kudo, 1933 (Myxosporea, Bivalvulida) infecting the gills of Labeo niloticus (Osteichthyes, Cyprinidae), were described for the first time from the River Nile at El...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360000270
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3654-2
更新日期：2014-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0448-9
更新日期：2007-06-01 00:00:00
abstract：:Parasites of managed bees can disrupt the colony success of the host, but also influence local bee-parasite dynamics, which is regarded as a threat for wild bees. Therapeutic measures have been suggested to improve the health of managed bees, for instance, exploiting the bees' RNA interference (RNAi) pathway to treat ...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 临床试验,杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00936184
更新日期：1992-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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更新日期：2019-12-01 00:00:00
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3974-x
更新日期：2014-08-01 00:00:00
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更新日期：2017-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00436-013-3709-4
更新日期：2014-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
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更新日期：2018-04-01 00:00:00
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更新日期：2011-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2009-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 历史文章,杂志文章,评审
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更新日期：2011-01-01 00:00:00
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journal_title：Parasitology research
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更新日期：2016-08-01 00:00:00
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更新日期：2018-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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abstract：:Nematodes and coccidia are major pathogens causing parasitic infections in livestock production. This study aimed to determine the prevalence and seasonality of gastrointestinal helminths and coccidia in communal sheep grazing in humid, semi-humid and arid zones of the Eastern Cape Province. A total of 3299 faecal sam...
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pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
abstract：:Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4844-x
更新日期：2016-03-01 00:00:00
abstract：:DNA primers were designed from the 18S rRNA sequence from the relevant digenean trematode Dicrocoelium dendriticum to evaluate a polymerase chain reaction-based diagnostic method of this parasite from its eggs in faeces of naturally and experimentally infected sheep. In order to get DNA from D. dendriticum eggs, sever...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3313-7
更新日期：2013-04-01 00:00:00
