Abstract:
:We have investigated how knowledge of endoplasmic reticulum (ER) retrieval signals can be used to study specific trafficking pathways in the malaria-infected erythrocyte. We show that addition of various lumenal ER retrieval signals to soluble green fluorescent protein (GFP) chimaera causes retrieval of the fusion protein in the parasite's ER. In contrast, adding these signals to the C-terminus of a membrane bound protein does not affect its eventual sub-cellular localization. This demonstrates proof of principle that ER retrieval signals can be used to study the solubility state of Plasmodium falciparum proteins during their transport to the host erythrocyte. Furthermore, using our knowledge of ER retrieval signals, we identify Plasmodium ER protein families and assign putative functions to them.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Külzer S,Gehde N,Przyborski JMdoi
10.1007/s00436-009-1397-xsubject
Has Abstractpub_date
2009-06-01 00:00:00pages
1535-41issue
6eissn
0932-0113issn
1432-1955journal_volume
104pub_type
杂志文章abstract::In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex p...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3526-9
更新日期:2013-10-01 00:00:00
abstract::The malaria parasite, Plasmodium falciparum, invades the human erythrocyte through a complex interaction with erythrocyte receptors characterized by patterns of resistance to various enzymes. As invasion rates are influenced by blood group polymorphisms, we reasoned that the extremely rare rhesus null (Rh(null)) eryth...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0756-8
更新日期:2008-01-01 00:00:00
abstract::Blastocystis hominis has been reclassified as a protozoan parasite. Its role as a human pathogen is somewhat controversial. There has been a dramatic increase in the frequency of B. hominis infection in association with diarrhea especially in immunocompromised hosts like AIDS patients, travelers, homosexuals, day care...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3652-4
更新日期:2014-01-01 00:00:00
abstract::Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its strong impact in public health, in this study, we determined the frequency of different Blastocystis subtypes in patients in France. We hypothesized on the mode of transmission and tested a possible relationship betwe...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1398-9
更新日期:2009-08-01 00:00:00
abstract::This study aims to observe the histopathological alterations in schistosomula of Schistosoma japonicum induced by mefloquine. Mice were infected with S. japonicum cercariae, and after 14 days, a single dose of mefloquine (400 mg/kg) was administered orally. After 8 h, 24 h, 3 days, 7 days, and 14 days, groups of two t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1611-x
更新日期:2009-11-01 00:00:00
abstract::Avian blood parasites have been shown to have significant health effects on avifauna worldwide. Sri Lanka, a tropical island rich with resident and migratory birds, has not been properly evaluated for avian blood parasites or their vectors. We investigated the presence of avian haemoparasites in Sri Lankan birds and t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-021-07049-3
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abstract::Feline lungworms infect the respiratory tract of wild and domestic cats, causing infection often associated with clinical disease. Until recently, Aelurostrongylus abstrusus has been considered the most relevant species of lungworm, while Troglostrongylus brevior was considered of less significance. Fecal samples of f...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06852-8
更新日期:2020-10-01 00:00:00
abstract::The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease b...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050435
更新日期:1998-06-01 00:00:00
abstract::For the identification of host species of blood-sucking parasites, the suitability of disc-electrophoresis of the stomach contents was tested. Mammalian blood in the stomach of the medicinal blood leech Hirudo medicinalis gave satisfactory results. In the case of mixed blood samples from H. medicinalis, the identifica...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931901
更新日期:1993-01-01 00:00:00
abstract::Thus far, Entamoeba species have been classified based on morphology such as the number of nuclei in mature cysts and their hosts. Using recently developed molecular tools, ruminant Entamoeba spp. are currently classified into four species/genotypes: E. bovis and Entamoeba ribosomal lineages (RL) 1, 2, and 4. However,...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5689-2
更新日期:2018-01-01 00:00:00
abstract::Cryptosporidium and Giardia are ubiquitous parasites that infect humans and animals. Few reports are available on the prevalence of these two protozoan parasites in yaks (Bos grunniens). In this study, 344 faecal samples were collected from yaks with diarrhoea in the Chenduo and Nangqian counties of Qinghai Province, ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5861-3
更新日期:2018-06-01 00:00:00
abstract::Hymenolepis diminuta is a zoonotic cestode parasitizing the small intestine of rodents (definitive hosts). Humans can accidentally enter into the life cycle of this tapeworm via the ingestion of infected insects (intermediate hosts) containing cestode cysticercoids in their body cavity. More than two centuries after t...
journal_title:Parasitology research
pub_type: 杂志文章,评审
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更新日期:2020-07-01 00:00:00
abstract::Excretory/secretory (ES) products collected from exsheathed L3 or parasitic stages of Haemonchus contortus were tested in vitro for gastrin stimulatory properties using an ovine abomasal antral mucosal preparation. In addition, the motility of exsheathed L3 and parasites recovered on weeks 2, 6 and 8 post-infection wa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1274-6
更新日期:2005-01-01 00:00:00
abstract::In the present study the behavior and pathogenicity of second-stage larvae of Toxocara canis were examined in different mouse strains with special emphasis on the major histocompatibility complex (MHC). Mice of the inbred strains BALB, C3H, C57BL, and DBA and the outbred strain NMRI were infected orally with 1000 seco...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932955
更新日期:1994-01-01 00:00:00
abstract::With the advent of polymerase chain reaction (PCR), genetic markers are now accessible for all organisms, including parasites. Amplified fragment length polymorphism (AFLP) is a PCR-based marker for the rapid screening of genetic diversity and intraspecific variation. It is a potent fingerprinting technique for genomi...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-012-3238-6
更新日期:2013-02-01 00:00:00
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journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00930906
更新日期:1991-01-01 00:00:00
abstract::Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive s...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5343-4
更新日期:2017-02-01 00:00:00
abstract::In life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. ...
journal_title:Parasitology research
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
abstract::Under natural conditions, eukaryote cells may contain bacteria. Arthropods such as ticks, insects or mites are a group particularly favoured by the obligate intracellular bacteria. While arthropods are vectors for some of them, other bacteria inhabit invertebrate host cells having entered mutualistic interactions. Suc...
journal_title:Parasitology research
pub_type: 杂志文章,评审
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更新日期:2007-04-01 00:00:00
abstract::This study assessed the anisakid nematode distribution pattern in the fish collected from coasts of Mediterranean Sea, Egypt, during the period September 2010-April 2011. Two hundred thirty out of 300 (76.7%) Dicentrarchus labrax (European seabass) marine fishes belonging to family Moronidae were dissected and found t...
journal_title:Parasitology research
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06808-y
更新日期:2020-09-01 00:00:00
abstract::P-Glycoprotein (P-GP) is a transport protein that participates in the mechanism of active secretion of different molecules from the bloodstream to the gastrointestinal tract. The aim of the current work was to evaluate the effect of verapamil, a P-GP substrate, on the pharmacokinetic behaviour of the anthelmintics ive...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1022-3
更新日期:2004-01-01 00:00:00
abstract::Micronemes are characteristic secretory organelles located within the apical cell region of apicomplexan parasites. The protein contents are exocytosed during an early phase of host cell invasion and contribute to parasite motility and the invasion of target cells. We report here on the cloning and heterologous expres...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0600-0
更新日期:2003-05-01 00:00:00
abstract::Three juvenile nematode parasites were collected naturally from 90 (75 %) out of 120 specimens of the marine greater lizard fish Saurida undosquamis captured from water coasts at Hurghada City along the Red Sea in Egypt during the period from September 2013 to April 2014. Worms were identified on the basis of light an...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4285-y
更新日期:2015-03-01 00:00:00
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journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00539573
更新日期:1988-01-01 00:00:00
abstract::The morphology, adult development and genetic characteristics of Echinococcus granulosus isolated from pigs in Poland were examined and compared with those of other recognised strains of E. granulosus. The isolates were characterised by their distinct morphology, rapid maturation and unique DNA hybridisation profiles....
journal_title:Parasitology research
pub_type: 杂志文章
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abstract::Gossypol, a natural polyphenolic compound, induces growth-inhibitory and antiparasitic effects in Taenia taeniaeformis metacestodes in vivo and in vitro. We investigated the uptake and localization of [3H]-gossypol in this parasite. Metacestodes were incubated in 10(-5) M [3H]-gossypol at 37 degrees C. Parasites stead...
journal_title:Parasitology research
pub_type: 杂志文章
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abstract::Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Babesia caballi and/or Theileria equi, which is endemic in many tropical and temperate areas of the world. However, clinical outbreaks of EP in Romania during the last decades have not been reported Therefore, the aim of this paper is (i) to describ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5899-2
更新日期:2018-07-01 00:00:00
abstract::The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0165-4
更新日期:2006-07-01 00:00:00
abstract::Although miltefosine (MIL) has only recently been positioned as a first-line therapeutic option for visceral leishmaniasis, field reports note an increasing trend in treatment failures. Study of laboratory selected MIL-resistant strains is needed in the absence of confirmed resistant clinical isolates. In contrast to ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4460-9
更新日期:2015-07-01 00:00:00