Abstract：
:This work describes a simple method to yield large amounts of Leishmania amastigote-like forms in axenic cultures using promastigotes as the starting population. The method described induced extracellular amastigote transformation of Leishmania amazonensis (97%), Leishmania braziliensis (98%) and Leishmania chagasi (90%). The rounded parasites obtained in axenic cultures were morphologically similar, even at the ultrastructural level, to intracellular amastigotes. Moreover, the axenic amastigotes remained viable as measured by their ability to revert back to promastigotes and to infect BALB/c mice. L. amazonensis and L. braziliensis promastigotes and axenic amastigotes differed in terms of their Western blot profiles. A 46 kDa protein was recognized by specific antibodies only in axenic and lesion-derived L. amazonensis amastigotes and not in promastigotes.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Teixeira MC,de Jesus Santos R,Sampaio RB,Pontes-de-Carvalho L,dos-Santos WLdoi
10.1007/s00436-002-0695-3subject
Has Abstractpub_date
2002-11-01 00:00:00pages
963-8issue
11eissn
0932-0113issn
1432-1955journal_volume
88pub_type
杂志文章abstract：:During investigations of fish parasites in the Neotropics (including the state of Veracruz and the Yucatán Peninsula in the Gulf of Mexico, the Chautengo Lagoon on the Pacific coast of the state of Guerrero in Mexico, and Lake Gatun in the Panama Canal), three monogenoidean (Dactylogyridae) species were found parasiti...
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