Abstract:
:An arbitrary-primed RNA PCR differential display strategy was used to identify midgut genes of the reduviid bug Triatoma infestans that were differentially expressed after a blood meal. From interesting bands, 33 distinct cDNAs were cloned and sequenced. Although many had long open reading frames, most of the transcripts were unrelated to any other sequences in any databases. Only 14 Triatoma sequences had strong homologies to those from other organisms, including genes encoding for 2-oxoglutarate dehydrogenase, CAD protein, NADH-ubiquinone-oxoreductase, epidermal growth factor, plectin, aminopeptidase, heat-shock-related 70-kDa protein, golgin, mitochondrial carrier protein and high-density lipoprotein. RT-PCR was used to demonstrate constitutive expression in four of five of these sequences. Northern hybridisation was difficult due to the very low expression levels of most of the genes. However, a gene-fragment highly homologous to a heat-shock-related 70-kDa protein was strongly expressed in starved bugs, down-regulated after feeding and again expressed later, suggesting a role for a heat-shock protein in starvation survival.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Kollien AH,Billingsley PFdoi
10.1007/s00436-002-0705-5subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1026-33issue
12eissn
0932-0113issn
1432-1955journal_volume
88pub_type
杂志文章abstract::Tick-borne intracellular protozoan parasites of the Theileria genus infect a wide range of both domestic and wild animals. In the present study, we describe the first PCR detection of Theileria luwenshuni in the blood of goats in Myanmar. Nested PCR targeting the Theileria 18S rRNA gene resulted in seven positive goat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6073-6
更新日期:2018-10-01 00:00:00
abstract::Schizothorax o'connori and Oxygymnocypris stewartii are two endangered endemic Tibetan fishes that thrive in the Lhasa River at an average altitude over 4000 m. During artificial reproduction of endemic Tibetan fishes, the juvenile fish of S. o'connori and O. stewartii experienced mass mortality event. The causative a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4527-7
更新日期:2015-08-01 00:00:00
abstract::Gyrodactylid monogeneans are considered to be the most invasive fish parasites. They are omnipresent on teleost fishes and host change might play a crucial role in their biology. In the present study, related fish species were kept in single- or mixed-species groups to test the ability of Gyrodactylus species to chang...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-0918-3
更新日期:2008-05-01 00:00:00
abstract::Three juvenile nematode parasites were collected naturally from 90 (75 %) out of 120 specimens of the marine greater lizard fish Saurida undosquamis captured from water coasts at Hurghada City along the Red Sea in Egypt during the period from September 2013 to April 2014. Worms were identified on the basis of light an...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4285-y
更新日期:2015-03-01 00:00:00
abstract::This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat s...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0851-x
更新日期:2008-04-01 00:00:00
abstract::This study was designed to provide a comprehensive description of the ultrastructure of the esophagus of Trichinella spiralis muscle larvae. Although the esophagus exhibited basically the same structure throughout its entire length, being composed of a single cell-layered epithelium, the basal lamina, and the cuticle,...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931189
更新日期:1988-01-01 00:00:00
abstract::We analyzed extra chromosomal genomes of avian blood protozoa, Leucocytozoon caulleryi. One of the genomes, the mitochondrial genome was completely sequenced resulting 5,959 bp in length. This genome contained the identical gene organization and contents to that of other avian blood protozoa previously analyzed: three...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1083-4
更新日期:2008-09-01 00:00:00
abstract::Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic mi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0763-8
更新日期:2003-06-01 00:00:00
abstract::Research on the infection of snails by trematodes has been conducted in Europe for over a hundred years. The initial poor knowledge of the intra-molluscan stages of these parasites together with the difficulty of classifying them constituted a serious obstacle to the undertaking of integrated parasitological and malac...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-009-1462-5
更新日期:2009-08-01 00:00:00
abstract::The authors previously reported that an extract from Zingiber officinale, traditionally eaten along with raw fish and used in traditional Chinese medicine, effectively destroyed Anisakis larvae in vitro. In this study, we analyzed the effective components of ginger rhizomes. Methanol extracts were fractionated after f...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931082
更新日期:1990-01-01 00:00:00
abstract::Members of the genus Crassicauda (Nematoda: Spirurida) are parasites of the body tissues of whales and dolphins. Owing to the large size of worms and difficulties in the recovery of entire nematodes from the tissues of hosts, limited information is available on morphological descriptions of both male and female worms....
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4245-6
更新日期:2015-03-01 00:00:00
abstract::Chagas disease, caused by the protozoan Trypanosoma cruzi (T. cruzi), although discovered more than a century ago, is still a not very well-elucidated aspect. Individuals in the chronic phase of the disease may present asymptomatic clinical form or symptomatologies related to the cardiac, digestive systems, or both (m...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06377-9
更新日期:2019-08-01 00:00:00
abstract::Eimeria spp. multiply within the intestinal tract causing severe inflammatory responses. Chitosan (CS), meanwhile, has been shown to exhibit anti-inflammatory activities in different experimental models. Here, we investigated the effect of CS on the outcome of inflammation caused by Eimeria papillata in the mouse inte...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5035-0
更新日期:2016-07-01 00:00:00
abstract::The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0165-4
更新日期:2006-07-01 00:00:00
abstract::In an experiment including 8 groups of 15 mice, the effect of migrating Ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to Pasteurella multocida was investigated. Following aerosol exposure to P. multocida, mice with migrating A. suum in their lungs developed more severe pne...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931575
更新日期:1992-01-01 00:00:00
abstract::Toxoplasmosis is a disease with a worldwide distribution that affects a wide variety of animal species, though with rare descriptions in chickens. We describe the clinical, epidemiological, pathological, and molecular aspects of a toxoplasmosis outbreak in domestic chickens and guinea fowl in southern Brazil. The floc...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-019-06233-w
更新日期:2019-03-01 00:00:00
abstract::Myxobolus miyairii Kudo, 1919 was first reported from the intestines of the Amur catfish (Silurus asotus) in Japan and then in China and Russia, but with incomplete description. During the investigation of fish myxosporean diversity in Poyang Lake, the biggest lake along the Yangtze River, China, two Amur catfish high...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4889-x
更新日期:2016-04-01 00:00:00
abstract::The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface prot...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050096
更新日期:1996-01-01 00:00:00
abstract::Host specificity is a fundamental property of parasites. Whereas most studies focus on measures of specificity on host range, only few studies have considered quantitative aspects such as infection intensity or prevalence. The relative importance of these quantitative aspects is still unclear, mainly because of method...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4154-8
更新日期:2014-12-01 00:00:00
abstract::The parasite community structure of the neotropical cormorant, Phalacrocorax brasilianus, from two lagoons (Coyuca and Tres Palos) from Guerrero state, México, was examined. Fourteen species of adult helminths (6,391 individuals) from 48 cormorants were identified: 9 digeneans, 1 acanthocephalan, 1 cestode, and 3 nema...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2377-5
更新日期:2011-11-01 00:00:00
abstract::Three monoclonal antibodies generated by immunization of mice with Plasmodium berghei-infected red blood cells were found to react with the 75-kDa heat-shock protein (HSP70) present in liver stages and erythrocytic forms of the parasites. These antibodies were shown to react with a recombinant protein encoding the car...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932618
更新日期:1994-01-01 00:00:00
abstract::Immunoperoxidase localisation of antigen at the electron microscope level confirms that parasite surface proteins, in association with membrane, are shed from the surface of the zoite on invasion, while varying amounts are also internalised. SAG 1 is stable on intracellular zoites for up to 48 h, although new protein ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931842
更新日期:1995-01-01 00:00:00
abstract::Spermatogenesis, testes, and vas deferens in the parthenogenetic monozoic tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea) from Slovakia, parasitizing the carp Cyprinus carpio L., have been investigated by means of transmission electron microscopy for the first time. The present results sh...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2038-0
更新日期:2011-01-01 00:00:00
abstract::The morphology of the scolex of the tapeworm Silurotaenica siluri (Batsch, 1786) (Proteocephalidae: Gangesiinae), a specific parasite of European wels (Silurus glanis L.), was studied using light, scanning (SEM), and transmission electron (TEM) microscopy. The scolex of S. siluri is rather uniform in shape and size; i...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050498
更新日期:1999-01-01 00:00:00
abstract::The diagnosis of toxoplasmosis is often complicated by the lack of specific clinical symptoms or postmortem features, in humans and other animals. The only diagnostic test described so far for the serological diagnosis of Toxoplasma gondii in marine mammals is the modified agglutination test (Dubey et al., Am J Vet Re...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3674-y
更新日期:2014-02-01 00:00:00
abstract::Naturally occurring bithyniid snails, Bithynia siamensis goniomphalos (Prosobranchia: Bithyniidae), and their intermediate hosts were sampled from Khammouane Province, the Lao People's Democratic Republic, and the prevalence of the carcinogenic human liver fluke, Opisthorchis viverrini, was examined. The presence of O...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2140-3
更新日期:2011-04-01 00:00:00
abstract::Tetratrichomonas didelphidis is a flagellate protozoan found in the intestine of the opossum. The parasite lives in a hostile and stressed environment, where it interacts with microorganisms and can survive under extreme conditions for growth, involving strict anaerobiosis or equilibration with air and abundance or ab...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1015-2
更新日期:2004-01-01 00:00:00
abstract::The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clo...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5249-1
更新日期:2016-12-01 00:00:00
abstract::The biochemical and structural changes that occur during the conversion of Toxoplasma gondii tachyzoites to bradyzoites and the formation of tissue cyst are not well understood. Maintaining cells infected with T. gondii type II and III strains under stress conditions induces the tachyzoite-bradyzoite in vitro differen...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5738-x
更新日期:2018-02-01 00:00:00
abstract::Hepatic enzymes and biochemical parameter values were analysed in the sera of 32 lambs: 12 infected with 1,000 Dicrocoelium dendriticum metacercariae, 12 with 3,000 and eight controls. Blood samples were collected at intervals of 30 days from day 0 post-infection (p.i.). Half the lambs in each group were slaughtered o...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1128-2
更新日期:2004-08-01 00:00:00