Differential display of mRNAs associated with blood feeding in the midgut of the bloodsucking bug, Triatoma infestans.

Abstract:

:An arbitrary-primed RNA PCR differential display strategy was used to identify midgut genes of the reduviid bug Triatoma infestans that were differentially expressed after a blood meal. From interesting bands, 33 distinct cDNAs were cloned and sequenced. Although many had long open reading frames, most of the transcripts were unrelated to any other sequences in any databases. Only 14 Triatoma sequences had strong homologies to those from other organisms, including genes encoding for 2-oxoglutarate dehydrogenase, CAD protein, NADH-ubiquinone-oxoreductase, epidermal growth factor, plectin, aminopeptidase, heat-shock-related 70-kDa protein, golgin, mitochondrial carrier protein and high-density lipoprotein. RT-PCR was used to demonstrate constitutive expression in four of five of these sequences. Northern hybridisation was difficult due to the very low expression levels of most of the genes. However, a gene-fragment highly homologous to a heat-shock-related 70-kDa protein was strongly expressed in starved bugs, down-regulated after feeding and again expressed later, suggesting a role for a heat-shock protein in starvation survival.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Kollien AH,Billingsley PF

doi

10.1007/s00436-002-0705-5

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

1026-33

issue

12

eissn

0932-0113

issn

1432-1955

journal_volume

88

pub_type

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