Electron microscopical investigation of surface alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody.

Abstract:

:The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface protein) and exhibits a complement-independent cytotoxic effect on trophozoites of clone GS/M-83-H7. Our investigations revealed that cytotoxicity of MAb G10/4 is associated with shedding of VSP-containing membrane vesicles from the parasite surface and a concomitant partial disruption of the cellular membrane. These morphological alterations depend on the cross-linking capacity of the antibody because the immunoreactivity of respective monovalent F(ab)' has no significant influence on the cell-surface structure. These findings indicate that the membrane-disintegrative activity of MAb G10/4 may be responsible for the parasitocidal function of the antibody.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Hemphill A,Stäger S,Gottstein B,Müller N

doi

10.1007/s004360050096

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

206-10

issue

3

eissn

0932-0113

issn

1432-1955

journal_volume

82

pub_type

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