Abstract:
:The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface protein) and exhibits a complement-independent cytotoxic effect on trophozoites of clone GS/M-83-H7. Our investigations revealed that cytotoxicity of MAb G10/4 is associated with shedding of VSP-containing membrane vesicles from the parasite surface and a concomitant partial disruption of the cellular membrane. These morphological alterations depend on the cross-linking capacity of the antibody because the immunoreactivity of respective monovalent F(ab)' has no significant influence on the cell-surface structure. These findings indicate that the membrane-disintegrative activity of MAb G10/4 may be responsible for the parasitocidal function of the antibody.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Hemphill A,Stäger S,Gottstein B,Müller Ndoi
10.1007/s004360050096subject
Has Abstractpub_date
1996-01-01 00:00:00pages
206-10issue
3eissn
0932-0113issn
1432-1955journal_volume
82pub_type
杂志文章abstract::Eggs obtained from the uteri of female nematodes, genetically identified as Contracaecum multipapillatum s.l., found in a brown pelican (Pelecanus occidentalis) from Bahía de La Paz, Gulf of California, Mexico, were used to study the early developmental stages of this anisakid by scanning electron microscopy (SEM). Eg...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5583-y
更新日期:2017-10-01 00:00:00
abstract::In vitro leucocyte proliferative responses to parasite antigens and to mitogens as well as lymphocyte sub-types were compared in guinea pigs with genetically determined differences in their ability to express protective immunity against Trichostronylus colubriformis infection. Proliferative responses to parasite antig...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050048
更新日期:2000-04-01 00:00:00
abstract::Resveratrol, a natural phytoalexin found mainly in grapes, possesses a variety of beneficial activities including anticancer, antimicrobial and antiviral. However, there is no information about its effects on kinetoplastid parasites such as Leishmania. Leishmania is a human pathogen responsible for a spectrum of disea...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0729-y
更新日期:2007-12-01 00:00:00
abstract::Adult worm extract from Ascaris suum (Asc) has immunosuppressive activity and elicits Th2/IL-4/IL-10 response. This study evaluated the prophylactic and therapeutic effect of Asc in a murine model of concanavalin A (ConA)-induced autoimmune hepatitis (AIH). BALB/c mice received ConA, iv, (20 mg/kg), and three groups o...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-3994-6
更新日期:2014-09-01 00:00:00
abstract::The malaria parasite, Plasmodium falciparum, invades the human erythrocyte through a complex interaction with erythrocyte receptors characterized by patterns of resistance to various enzymes. As invasion rates are influenced by blood group polymorphisms, we reasoned that the extremely rare rhesus null (Rh(null)) eryth...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0756-8
更新日期:2008-01-01 00:00:00
abstract::Culex pipiens pallens is the mosquito vector of a number of human pathogens such as Wuchereria bancrofti, Brugia malayi, and epidemic encephalitis B virus. Female C. pipiens pallens play an important role in transmitting pathogens by sucking blood, which is essential for reproduction. In the present study, a subtracti...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0506-y
更新日期:2007-08-01 00:00:00
abstract::The CSP, EMP2/MESA, MSP2, MSP3, MSP5, RAP1, RAP2, RESA1, SERA1 and SSP2/TRAP genes of Plasmodium falciparum are vaccine candidates. The hepatic phase of the infection is of major interest due to the protection induced by immunization with radiation-attenuated sporozoites. We therefore performed RT-PCR experiments to d...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1061-9
更新日期:2004-04-01 00:00:00
abstract::Thelohania montirivulorum sp. nov., a new species of microsporidian parasite, was found in a highland population of the Australian yabby, Cherax destructor. Data are presented on fine ultrastructure, developmental morphology and DNA sequence of the small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-0948-9
更新日期:2003-10-01 00:00:00
abstract::The uptake of amino acids is mediated by active transporters located on the basolateral and brush border membranes of intestinal epithelial cells. The current study investigated the expression of amino acid transporters (AAT) and other genes in the intestine of chicks infected with Eimeria maxima. At 7-day postinfecti...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4114-3
更新日期:2014-10-01 00:00:00
abstract::The infectivity of Cryptosporidium muris (strain RN 66), originally isolated from the house rat (Iseki 1986), to various laboratory animals was studied by transmission experiments. After oral inoculation with 1 x 10(6) oocysts, mice, guinea pigs, rabbits, dogs, and cats all discharged endogenously produced oocysts in ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931279
更新日期:1989-01-01 00:00:00
abstract::We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyp...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1304-x
更新日期:2009-03-01 00:00:00
abstract::A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 inte...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1006-3
更新日期:2003-12-01 00:00:00
abstract::A quantitative colorimetric assay using the oxidation-reduction indicator resazurin was developed to measure cytotoxicity of compounds against the protozoan parasite Trypanosoma cruzi. This method is based on the detection of colorimetric changes caused by the oxidation (blue) and reduction (pink) capabilities of resa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0126-y
更新日期:2006-07-01 00:00:00
abstract::Hymenolepis diminuta is a zoonotic cestode parasitizing the small intestine of rodents (definitive hosts). Humans can accidentally enter into the life cycle of this tapeworm via the ingestion of infected insects (intermediate hosts) containing cestode cysticercoids in their body cavity. More than two centuries after t...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-020-06663-x
更新日期:2020-07-01 00:00:00
abstract::The activities of the disaccharidases lactase, maltase, sucrase and trehalase were examined in gerbils during Giardia lamblia infections. In a primary infection with trophozoites, the activities of all four enzymes were reduced from day 10 post-infection (p.i.) and remained at low levels well past the elimination phas...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932025
更新日期:1995-01-01 00:00:00
abstract::Antimicrobial peptides are increasingly being explored as alternative agents for therapy against the parasitic protozoan Leishmania. Previously, we reported that the synthetic magainin analog, pexiganan, induced apoptosis of surface protease-deficient Leishmania. Here, we report the development of an arginine-rich var...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-3847-3
更新日期:2014-05-01 00:00:00
abstract::Several polymorphic markers, including serine-rich protein genes, have been used for the genotyping of isolates from the morphologically indistinguishable protozoan parasites Entamoeba histolytica, Entamoeba dispar, and Entamoeba nuttalli. Genotypes of tRNA-linked short tandem repeats (STRs) are highly polymorphic, bu...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3664-0
更新日期:2014-01-01 00:00:00
abstract::To seek natural products for the development of environment friendly mosquito control agents, fumigant activity of eleven essential oil compounds and the joint action of the active compounds were evaluated against Culex pipiens pallens adults. Fumigant bioassay demonstrated that carvacrol exhibited the highest fumigan...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4028-0
更新日期:2014-10-01 00:00:00
abstract::The effect of pentavalent meglumine antimoniate (glucantime) on the growth kinetics of promastigotes of 13 South American Leishmania strains isolated from patients, sylvatic reservoirs, and vectors was studied. Four of five L. (Viannia) braziliensis human isolates were obtained from drug-responsive patients and one wa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050476
更新日期:1998-09-01 00:00:00
abstract::The effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. This is due to the fact that some invasive mosquitoes, with special reference to the Aedes genus, are particularly difficult to control, due to their high ecological plasticity. Moreover, the indiscriminate over...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5395-0
更新日期:2017-04-01 00:00:00
abstract::Common helminth infections promote Th2-skewed immune responses in their hosts. We have studied the role of intact carbohydrate structures on Taenia crassiceps compounds in the induction of biased type 2 and anti-inflammatory immune responses on peptide-stimulated T cells by using DO11.10 transgenic (OVA Tg) mice. Whil...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0159-2
更新日期:2006-09-01 00:00:00
abstract::Experimental concurrent infection with two or more parasite species in mammalian host models may result in heterologous antagonistic and synergistic interactions ranging in magnitude from reduced/enhanced growth and fecundity to blockage/enhancement of establishment/expulsion. With some exceptions only, there is a rea...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/BF00538196
更新日期:1987-01-01 00:00:00
abstract::In many helminthic infections, eotaxin, a CC-chemokine, triggers the mobilization of eosinophils, thus, contributing to an elevated blood and periparasitic eosinophil level. Following an experimental intraperitoneal infection of C57BL6 mice with Echinococcus multilocularis metacestodes, however, we observed the absenc...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1549-z
更新日期:2009-10-01 00:00:00
abstract::This report describes a technique for studying the adherence and growth of Giardia intestinalis trophozoites (strains PARIS/86/LCF/1, PARIS/86/LCF/2 and PARIS/88/LCF/8) using the human colon carcinoma cell line Caco2. Giardia trophozoites were cultured with Caco2 cells in a modified HSP3 culture medium. The biochemica...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932566
更新日期:1990-01-01 00:00:00
abstract::The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0810-6
更新日期:2008-03-01 00:00:00
abstract::For years, mammals of the order Pilosa have been considered Leishmania reservoirs. But while most studies have focused on sloth species, anteaters have been overlooked, and in many Leishmania endemic countries like Mexico, no studies have been carried out. The aims of this work were to identify the presence of Leishma...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-019-06253-6
更新日期:2019-04-01 00:00:00
abstract::Babesia motasi BQ1 (Lintan) was first isolated from Haemaphysalis qinghaiensis collected in Gannan Tibet Autonomous Region, Gansu province in April 2000. In this study, a total of 3,204 serum samples from small ruminants in 22 provinces located in different districts of China were tested for antibodies against merozoi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3310-x
更新日期:2013-06-01 00:00:00
abstract::Potent compounds do not necessarily make the best drugs in the market. Consequently, with the aim to describe tools that may be fundamental for refining the screening of candidates for animal and preclinical studies and further development, molecules of different structural classes synthesized within the frame of a br...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4210-4
更新日期:2015-02-01 00:00:00
abstract::Numerous nematodes were found in the rectum of three fish species Synodontis ocellifer, S. nigrita, and S. schall (Mochokidae, Siluriformes) from the Gambia River and Mare Simenti, National Park Niokolo Koba, Senegal. A nematode species Raillietnema synodontisi Vassiliadès, 1973 (host S. ocellifer), is redescribed usi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0869-0
更新日期:2008-04-01 00:00:00
abstract::The purpose of our study was to assess the effects of experimental dicroceliosis on the antioxidant defense capability of the liver in hamsters. Studies were carried out at 80 and 120 days after infection with an oral dose of 40 metacercariae of Dicrocoelium dendriticum. The parasitic pathology was ascertained by the ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050579
更新日期:1999-06-01 00:00:00