Abstract：
:The present study aimed to isolate and genotype strains of T. gondii from pigs slaughtered for human consumption in South Brazil. Blood and tissues (heart, diaphragm, liver, tongue, and masseter) from 400 animals were collected at two slaughterhouses. Sera were obtained, and antibodies against T. gondii were detected by both indirect fluorescence antibody test (IFAT) and modified agglutination test (MAT). The tissues of animals that tested positive in MAT, IFAT, or both (cut-off ≥ 64) were bioassayed. Twenty-six (6.5%) of the 400 animals were positive by serology. A total of 18 (69.2%) out of those 26 were positive in the mouse bioassay. The isolates were characterized by using 10 PCR-RFLP genetic markers. Fourteen isolates were fully genotyped, and four isolates were genotyped using nine of the 10 markers. All isolates belonged to ToxoDB PCR-RFLP genotype #206. The present study reports on genotype #206 in pigs for the first time, and it confirms the atypical nature of the Brazilian T. gondii isolates. Additionally, even with low levels of antibodies detected in pig herds, pork presents a T. gondii infection risk for humans.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Miura AC,de Barros LD,Ferreira FP,Neto JMF,Sicupira Franco PML,Su C,Vidotto O,Garcia JLdoi
10.1007/s00436-019-06274-1subject
Has Abstractpub_date
2019-05-01 00:00:00pages
1593-1599issue
5eissn
0932-0113issn
1432-1955pii
10.1007/s00436-019-06274-1journal_volume
118pub_type
杂志文章abstract：:We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyp...
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pub_type： 杂志文章,随机对照试验
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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