Molecular cloning and characterization of a novel ras-related protein (rap2) from Clonorchis sinensis.


:Ras are key components of diverse signal transduction pathways and play important roles in growth and development. To know about growth regulation in Clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cDNA library. The open reading frame contains 561 bp encoding 186 amino acids. The hypothetical amino acid sequence shared high identities with rap2 proteins from Schistosoma japonicum and Homo sapiens. Conserved domains of small guanosine triphosphate-binding proteins and characteristic amino acid residues of rap2 proteins were observed in this sequence. Reverse transcription polymerase chain reaction experiments revealed that rap2 transcribed in adult worm, metacercaria, and eggs of C. sinensis. Recombinant rap2 protein was expressed and purified from Escherichia coli. rap2 could be probed by C. sinensis-infected rat serum in western blotting experiment. By immunohistochemistry, rap2 was localized on the tegument of adult worm and metacercaria of C. sinensis. This fundamental study might contribute to further researches in signaling systems that are related to growth control and development of C. sinensis and other parasites.


Parasitol Res


Parasitology research


Chen W,Wang X,Deng C,Lv X,Fan Y,Men J,Liang C,Yu X




Has Abstract


2011-04-01 00:00:00












  • Tortoise tick Hyalomma aegyptium as long term carrier of Q fever agent Coxiella burnetii--evidence from experimental infection.

    abstract::The experimental study investigated the ability of tortoise tick Hyalomma aegyptium to play a role in forming and maintaining natural foci of Q fever. We tested the competence of H. aegyptium larvae to acquire Coxiella burnetii infection from mammals, serve as a C. burnetii vector between mammalian hosts, and be a lon...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Siroký P,Kubelová M,Modrý D,Erhart J,Literák I,Spitalská E,Kocianová E

    更新日期:2010-11-01 00:00:00

  • Diagnosis of Plasmodium falciparum malaria in pregnancy in sub-Saharan Africa: the challenges and public health implications.

    abstract::Plasmodium falciparum malaria in pregnancy is a major public health problem in sub-Saharan Africa and represents enormous diagnostic challenge. The objective of this report was to review scientific data from studies conducted in sub-Saharan Africa on the diagnosis of malaria in pregnancy within the last two decades (1...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Uneke CJ

    更新日期:2008-02-01 00:00:00

  • The first detection of Cryptosporidium deer-like genotype in cattle in Japan.

    abstract::The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C. parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Amer S,Honma H,Ikarashi M,Oishi R,Endo M,Otawa K,Nakai Y

    更新日期:2009-03-01 00:00:00

  • Evaluation of larvicidal activity of Acalypha alnifolia Klein ex Willd. (Euphorbiaceae) leaf extract against the malarial vector, Anopheles stephensi, dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefasciatus (Diptera: Culicid

    abstract::The leaf extract of Acalypha alnifolia with different solvents - hexane, chloroform, ethyl acetate, acetone and methanol - were tested for larvicidal activity against three important mosquitoes such as malarial vector, Anopheles stephensi, dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kovendan K,Murugan K,Vincent S

    更新日期:2012-02-01 00:00:00

  • Information-based methods in the development of antiparasitic drugs.

    abstract::The discovery and optimization of antiparasitic compounds has profited by information-based methods newly emerged in the modern drug development process. The generation of computer models enables the cost-efficient and fast computational screening of virtual compound libraries for biologically active molecules. Two so...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Wolf K,Dormeyer M

    更新日期:2003-06-01 00:00:00

  • Development of an in vitro system to study the developmental stages of Toxoplasma gondii using a genetically modified strain expressing markers for tachyzoites and bradyzoites.

    abstract::Toxoplasma gondii, the agent of toxoplasmosis, is an intracellular parasite that can infect a wide range of vertebrate hosts. Toxoplasmosis causes severe damage to immunocompromised hosts and its treatment is mainly based on the combination of pyrimethamine and sulfadiazine, which causes relevant side effects primaril...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Portes JA,De Souza W

    更新日期:2019-12-01 00:00:00

  • A case of human orotracheal myiasis caused by Wohlfahrtia magnifica.

    abstract::A case of orotracheal myiasis in an 80-year-old man in coma for 1 week in the intensive care unit of Baskent University Hospital, Ankara, Turkey, is reported. A number of larvae of Wohlfahrtia magnifica were recovered repeatedly from the mouth and from the intubation tube of the patient and were reared to adults to fa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ciftçioğlu N,Altintaş K,Haberal M

    更新日期:1997-01-01 00:00:00

  • Metazoan parasites of Micropterus salmoides (Lacépède 1802) (Perciformes, Centrarchidae): a review with evidences of spillover and spillback.

    abstract::Among the topics related to invasion science, the least studied are parasite co-introduction and spillback. This leads to an uncertainty in invasion ecology theories and applications to management. Therefore, the present study brings a systematic review of published information on the metazoan parasite fauna of Microp...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Costa APL,Takemoto RM,Vitule JRS

    更新日期:2018-06-01 00:00:00

  • The field efficacy of garlic extract against Dermanyssus gallinae in layer farms of Babol, Iran.

    abstract::Dermanyssus gallinae (Acari: Mesostigmata) is the most important hematophagous ectoparasite in layer farms in many countries. The reproduction rate of the parasite is rapid and can be completed in a week under favorable conditions. The parasite has direct and indirect effects on birds. It can also act as a vector for ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Faghihzadeh Gorji S,Faghihzadeh Gorji S,Rajabloo M

    更新日期:2014-03-01 00:00:00

  • Susceptibility to leishmaniasis is affected by host SLC11A1 gene polymorphisms: a systematic review and meta-analysis.

    abstract::Leishmaniases are cutaneous, mucocutaneous, and visceral diseases affecting humans and domesticated animals mostly in the tropical and subtropical areas of the planet. Host genetics have been widely investigated for their role in developing various infectious diseases. The SLC11A1 gene has been reported to play a role...

    journal_title:Parasitology research

    pub_type: 杂志文章,meta分析


    authors: Braliou GG,Kontou PI,Boleti H,Bagos PG

    更新日期:2019-08-01 00:00:00

  • Anthelmintic activities of chloroform and methanol extracts of Buchholzia coriacea Engler seed.

    abstract::The anthelmintic potentials of the chloroform and methanol extracts of Buchholzia coriacea Engler seed were investigated. In folklore medicine, B. coriacea (Capparidaceae) is believed to be useful in the treatment of various kinds of ailments and diseases. At doses of 10 mg/kg, 25 mg/kg and 50 mg/kg, the extracts were...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Fred-Jaiyesimi AA,Adepoju A,Egbebunmi O

    更新日期:2011-08-01 00:00:00

  • In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus).

    abstract::In search of a natural antiparasitic, in vivo anthelmintic activity of petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts of Angelica pubescens roots (Radix angelicae pubescentis), Brucea javanica fruits (Fructus bruceae), Spatholobus suberectus stems (Caulis spatholobi), Aesculus chinensis Bge...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu YT,Wang F,Wang GX,Han J,Wang Y,Wang YH

    更新日期:2010-04-01 00:00:00

  • The tapeworm Atractolytocestus tenuicollis (Cestoda: Caryophyllidea)--a sister species or ancestor of an invasive A. huronensis?

    abstract::Atractolytocestus tenuicollis (Li, 1964) Xi, Wang, Wu, Gao et Nie, 2009 is a monozoic, non-segmented tapeworm of the order Caryophyllidea, parasitizing exclusively common carp (Cyprinus carpio L.). In the current work, the first molecular data, in particular complete ribosomal internal transcribed spacer 2 (ITS2) and ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Králová-Hromadová I,Štefka J,Bazsalovicsová E,Bokorová S,Oros M

    更新日期:2013-10-01 00:00:00

  • Molecular characterization of the histone H2A gene from the parasite Trypanosoma rangeli.

    abstract::The sequence, genomic organization, and transcription of the gene encoding the H2A histone protein of the protozoan parasite Trypanosoma rangeli is described in this paper. The locus encoding the T. rangeli H2A protein is formed by at least 11 gene units measuring 790 nucleotides in length, organized in tandem, and lo...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Puerta C,Cuervo P,Thomas MC,Lopez MC

    更新日期:2000-11-01 00:00:00

  • The putative telomerase reverse transcriptase component of Leishmania amazonensis: gene cloning and characterization.

    abstract::The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence al...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Giardini MA,Lira CB,Conte FF,Camillo LR,de Siqueira Neto JL,Ramos CH,Cano MI

    更新日期:2006-04-01 00:00:00

  • Ancient parasites from endemic deer from "CUEVA PARQUE DIANA" archeological site, Patagonia, Argentina.

    abstract::The narrow Andean-Patagonian temperate rainforest strip in the west of southern South America is inhabited by two endemic species of cervids, the southern pudu (Pudu puda) and the huemul (Hippocamelus bisulcus), both cataloged as near threatened and threatened species, respectively. One of the possible causes of their...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Beltrame MO,Tietze E,Pérez AE,Bellusci A,Sardella NH

    更新日期:2017-05-01 00:00:00

  • Distribution of Anopheles mosquito species, their vectorial role and profiling of knock-down resistance mutations in Botswana.

    abstract::Knowledge of vector species composition and monitoring their change over time is critical to evaluate malaria transmission and assess control interventions. This is especially important in countries such as Botswana, where malaria transmission is subjected to fluctuations due to climate variability. Another important ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kgoroebutswe TK,Ramatlho P,Reeder S,Makate N,Paganotti GM

    更新日期:2020-04-01 00:00:00

  • Intraspecific and interspecific genetic variation of Gongylonema pulchrum and two rodent Gongylonema spp. (G. aegypti and G. neoplasticum), with the proposal of G. nepalensis n. sp. for the isolate in water buffaloes from Nepal.

    abstract::The gullet worm (Gongylonema pulchrum) has been recorded from a variety of mammals worldwide. In an earlier study, we demonstrated two separate transmission cycles in cattle (Bos taurus) and wild mammals in Japan based on nucleotide sequences of the ribosomal RNA gene (rDNA) and cytochrome c oxidase subunit I (cox-1) ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Setsuda A,Da N,Hasegawa H,Behnke JM,Rana HB,Dhakal IP,Sato H

    更新日期:2016-02-01 00:00:00

  • The effect of dietary antioxidant supplementation in a vertebrate host on the infection dynamics and transmission of avian malaria to the vector.

    abstract::Host susceptibility to parasites is likely to be influenced by intrinsic factors, such as host oxidative status determined by the balance between pro-oxidant production and antioxidant defences. As a result, host oxidative status acts as an environmental factor for parasites and may constrain parasite development. We ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Delhaye J,Glaizot O,Christe P

    更新日期:2018-07-01 00:00:00

  • The effect of dimethylsulfoxide on the tegumental brush border of the cestode Hymenolepis diminuta.

    abstract::Dimethyl sulfoxide (DMSO), commonly used for cryoprotection or for the solvation of cytoskeleton-modifying drugs, causes changes in the topology of the plasma membrane of the tegumental brush border in the tapeworm Hymenolepis diminuta; however, relatively long exposures of high concentrations are required. In tapewor...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Forman LA,Oaks JA

    更新日期:1992-01-01 00:00:00

  • Spatial distribution and seasonality of Biomphalaria spp. in São Luís (Maranhão, Brazil).

    abstract::Two of the three vector species of Schistosoma mansoni Sambon, 1907 in Brazil occur in the state of Maranhão: Biomphalaria glabrata (Say, 1818) and Biomphalaria straminea (Dunker, 1848). For the implementation of effective measures to combat schistosomiasis, it is necessary to identify the spatial and seasonal dynamic...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: David NF,Cantanhede SPD,Monroe NB,Pereira LPLA,Silva-Souza N,Abreu-Silva AL,de Oliveira VM,Tchaicka L

    更新日期:2018-05-01 00:00:00

  • Ultrastructural observations of the attachment organs of the monogenean Rajonchocotyle emarginata (Olsson, 1876) (Polyopisthocotylea: Hexabothriidae), a gill parasite of rays.

    abstract::The present study uses scanning and transmission electron microscopical observations to examine the haptoral features of the hexabothriid polyopisthocotylean monogenean Rajonchocotyle emarginata from the gills of the elasmobranch Amblyraja radiata. The haptor possesses six equal, pedunculate suckers, each armed with a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Poddubnaya LG,Hemmingsen W,Gibson DI

    更新日期:2016-06-01 00:00:00

  • Sarcophaga (Liosarcophaga) tibialis Macquart 1851 (Diptera: Sarcophagidae): micromorphology of preimaginal stages of a fly of medical and veterinary interest.

    abstract::Sarcophagids are a large family of Diptera, with a worldwide distribution. They are related to decomposing organic matter and are very interesting for health science and in forensic cases since many species produce myiasis and occur in human corpses. This family is considered difficult to study, particularly with rega...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Paños-Nicolás Á,Arnaldos MI,García MD,Ubero-Pascal N

    更新日期:2015-11-01 00:00:00

  • Intestinal microsporidia infection among cat owners and non-pet owners in Iran: a case-control study.

    abstract::Microsporidia is a group of spore-forming microorganisms with zoonotic potential. This study aimed to compare intestinal microsporidia infections in cat owners and non-pet owners. In total, 210 fecal samples were collected from indoor cats, cat owners, and non-pet owners. DNA extraction was performed and the small sub...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Vahedi SM,Jamshidi S,Shayan P,Bokaie S,Ashrafi Tamai I,Javanmard E,Mirjalali H

    更新日期:2020-06-01 00:00:00

  • Asian fish tapeworm, Khawia japonensis (Yamaguti, 1934), has expanded its European invasive range.

    abstract::The invasive fish tapeworm, Khawia japonensis (Yamaguti, 1934) originally described in Japan, is reported for the first time in Slovakia. The tapeworm was found in farmed common carp (Cyprinus carpio L.) from East-Slovakian breeding fish pond. This finding is registered 4 years after the first announcement of this par...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Oros M,Barčák D,Bazsalovicsová E,Hanzelová V

    更新日期:2015-05-01 00:00:00

  • Structure of parasites community in Chaetobranchopsis orbicularis (Cichlidae), a host from the Amazon River system in northern Brazil.

    abstract::The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Tavares-Dias M,Oliveira MSB

    更新日期:2017-08-01 00:00:00

  • Vector movement underlies avian malaria at upper elevation in Hawaii: implications for transmission of human malaria.

    abstract::With climate warming, malaria in humans and birds at upper elevations is an emerging infectious disease because development of the parasite in the mosquito vector and vector life history are both temperature dependent. An enhanced-mosquito-movement model from climate warming predicts increased transmission of malaria ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Freed LA,Cann RL

    更新日期:2013-11-01 00:00:00

  • Detection of Blastocystis from stool samples using real-time PCR.

    abstract::We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blas...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Jones MS 2nd,Ganac RD,Hiser G,Hudson NR,Le A,Whipps CM

    更新日期:2008-08-01 00:00:00

  • Larvicidal, ovicidal, and adulticidal efficacy of Erythrina indica (Lam.) (Family: Fabaceae) against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae).

    abstract::Mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis, and Japanese encephalitis. Mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative b...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Govindarajan M,Sivakumar R

    更新日期:2014-02-01 00:00:00

  • Do parasites influence behavioural traits of wild and hatchery-reared Murray cod, Maccullochella peelii?

    abstract::This study aimed to investigate the links between parasites and behavioural traits of juvenile Murray cod (Maccullochella peelii). The Murray cod is an endangered Australian freshwater fish for which restocking programs are in place and there is a growing human consumption market. However, little is known about the pa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Shamsi S,Rogers L,Sales E,Kopf RK,Freire R

    更新日期:2021-01-06 00:00:00