Abstract:
:Acanthamoeba are found in the environment, particularly in water, all over the world. The genus is currently classified into 20 different genotypes, T1-T20. In this study, 63 natural water samples from 11 provinces in northeast Thailand were collected and cultured on non-nutrient agar plates. Positive samples by culture were subsequently analyzed by molecular methods. The identification of Acanthamoeba was based on morphological features and molecular techniques using PCR and DNA sequencing. The results showed that 10 samples out of 63 were positive (15.9 %). Phylogenetic analysis revealed that seven samples were T4, one sample was similar to T3, and the other two samples were similar to T5. This is the first report demonstrating the contamination of Acanthamoeba species in natural water sources in northeast Thailand.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Thammaratana T,Laummaunwai P,Boonmars T,Neglected, zoonosis and vector-borne disease research group.doi
10.1007/s00436-016-4911-ysubject
Has Abstractpub_date
2016-04-01 00:00:00pages
1705-9issue
4eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-4911-yjournal_volume
115pub_type
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