Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore.

Abstract:

:Blastocystis is an enteric protozoan parasite commonly found in humans and animals. Phylogenetic and genotypic analyses have shown that Blastocystis exhibits extreme genetic diversity, and humans are host to a number of zoonotic isolates. In the present study, the prevalence of Blastocystis in 276 stool samples from a hospital in Singapore was examined, and for the first time, riboprinting using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the genetic diversity of the Blastocystis isolated from the Singapore population. The prevalence rate was determined to be 3.3% (9/276), and Blastocystis displaying two main ribotypes were isolated. As a comparison, we performed PCR-RFLP using two different published methodologies, and both methods allowed the isolates to be divided into two distinct groups based on their riboprint patterns. According to a recently proposed classification scheme, 78% (7/9) of the isolates were of subtype 3, while 22% (2/9) were subtype 1. The predominance of subtype 3 in an urbanized city state such as Singapore is in agreement with the idea that subtype 3 is a genotype of human origin.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Wong KH,Ng GC,Lin RT,Yoshikawa H,Taylor MB,Tan KS

doi

10.1007/s00436-007-0808-0

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

663-70

issue

4

eissn

0932-0113

issn

1432-1955

journal_volume

102

pub_type

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