Abstract：
:The localization and distribution of SALMFamide (S1)-like immunoreactivity (IR), was determined at both the cellular and subcellular level in the central nervous system (CNS) of the nematode roundworm Ascaris suum. The techniques of indirect immunofluorescence in conjunction with confocal scanning laser microscopy and post-embedding, IgG-conjugated colloidal gold immunostaining were used, respectively. Immunostaining was widespread in the CNS of adult A. suum, with immunoreactivity (IR) being localized in nerve cells and fibres in the ganglia associated with the anterior nerve ring and in the main nerve cords and their commissures. At the subcellular level, gold labeling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the neuropile of the anterior nerve ring, main ganglia and nerve cords in the CNS. Double-labeling demonstrated an apparent co-localization of S1- and FMRFamide-IR-together with S1- and pancreatic polypeptide (PP)-IR in the same dense-cored vesicles. Antigen preabsorption experiments indicated little cross-reactivity, if any, between the three antisera; indeed, neither FMRFamide nor PP antigens abolished S1 immunostaining.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Brownlee DJ,Fairweather I,Thorndyke MC,Johnston CFdoi
10.1007/s004360050086subject
Has Abstractpub_date
1996-01-01 00:00:00pages
149-56issue
2eissn
0932-0113issn
1432-1955journal_volume
82pub_type
杂志文章abstract：:Ticks and tick-borne diseases (TBDs) continue to pose an insidious and ever-present threat to livestock and livelihoods across the globe. Two of the most significant TBDs of cattle in Africa are heartwater and babesioisis, caused by Ehrlichia ruminantium and Babesia bigemina respectively. Both pathogens are endemic in...
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pub_type： 杂志文章,评审
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更新日期：2008-12-01 00:00:00
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更新日期：2010-05-01 00:00:00
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更新日期：2010-11-01 00:00:00
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更新日期：2007-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2009-11-01 00:00:00
