Abstract：
:Murine immunological responses to experimental infection with Trichuris muris and the effects of the resident microbiota on these responses are of increasing interest. For these studies, accurate dose delivery and improved sterilization of inocula are essential to prevent co-infection with unknown contaminants. We found that washing T. muris eggs with antibiotics may not be sufficient for sterilization of inocula. However, washing eggs in 6.25% hypochlorite/bleach eliminated bacteria and fungi, as determined by culture and PCR, did not harm viable T. muris eggs and reduced the number of non-viable eggs in the inocula. A hatching assay and propidium iodide staining method were developed and found to increase the accuracy for assessing T. muris egg viability prior to infection for rapid dose evaluation. In addition, metal gavage feeding needles increased the accuracy and precision of the dose delivered to the mice compared to flexible rubber tubes. These methods will improve experimental Trichuris studies by decreasing the variability in outcome due to unintended carryover of adherent microorganisms and unrecognized variation in inocula.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Kopper JJ,Mansfield LSdoi
10.1007/s00436-010-1978-8subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1103-13issue
5eissn
0932-0113issn
1432-1955journal_volume
107pub_type
杂志文章abstract：:The oviposition deterrent and ovicidal of seven herbal essential oils derived from Citrus sinensis, Cymbopogon citratus, Eucalyptus glubulus, Illicium verum, Lavandula angustifolia, Mentha piperita, and Zingiber cussumunar were assessed against the gravid female of housefly, Musca domestica L., under laboratory condit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3964-z
更新日期：2014-08-01 00:00:00
abstract：:Chagas disease, caused by the protozoan Trypanosoma cruzi, is considered to be a multifactorial disease associated with host and parasite genetics, which influence clinical aspects of the disease and other host conditions. In order to understand better the evolution of the disease, this study intended to evaluation of...
journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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doi：10.1007/s00436-015-4649-y
更新日期：2015-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00535326
更新日期：1987-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2321-8
更新日期：2011-09-01 00:00:00
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更新日期：2003-10-01 00:00:00
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更新日期：2017-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00539573
更新日期：1988-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050175
更新日期：1996-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931812
更新日期：1989-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0781-7
更新日期：2008-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00436-007-0552-5
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-08-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
abstract：:Cattle tick (Rhipicephalus microplus) is an important constraint on livestock production, particularly in tropical and subtropical areas. Synthetic acaricides are the primary method of control this thick. Resistance of R. microplus to synthetic acaricides has given rise to the need of new scientific investigations on ...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931279
更新日期：1989-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4949-x
更新日期：2016-05-01 00:00:00
abstract：:The widespread apicomplexan parasites Toxoplasma gondii (T. gondii) and Eimeria tenella (E. tenella) are important pathogens with high prevalence in poultry. The aim of our study was the investigation of mutual influences in co-infected chickens, focusing on immune response and course of infection. Two separate trials...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5636-2
更新日期：2017-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06514-4
更新日期：2020-01-01 00:00:00
abstract：:The control of body lice is an important issue for human health and welfare because lice act as vectors of disease such as typhus, relapsing fever, and trench fever. Body lice exhibit avoidance behavior to some essential oils, including clove essential oil. Therefore, odorants containing clove essential oil components...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4905-9
更新日期：2016-04-01 00:00:00
