Abstract：
:In an effort to identify novel therapeutic alternatives for the treatment of malaria, the present study evaluated the antimalarial effect of the crude hydroalcoholic extract (HCE) from the leaves of Chenopodium ambrosioides L. For this purpose, the molecular affinity between the total proteins from erythrocytes infected with Plasmodium falciparum and HCE or chloroquine was evaluated by surface plasmon resonance (SPR). Subsequently, the plasmodicidal potential of HCE was assessed in a P. falciparum culture. Using BALB/c mice infected with Plasmodium berghei intraperitoneally (ip.), we evaluated the effects of ip. treatment, for three consecutive days (day 7, 8, and 9 after infection), with chloroquine (45 mg/kg) or HCE (5 mg/kg), considering the survival index and the parasitaemia. The groups were compared to an untreated control group that receives only PBS at the same periods. The results indicated that HCE could bind to the total proteins of infected erythrocytes and could inhibit the parasite growth in vitro (IC50 = 25.4 g/mL). The in vivo therapeutic treatment with HCE increased the survival and decreased the parasitaemia in the infected animals. Therefore, the HCE treatment exhibited a significant antiplasmodial effect and may be considered as a potential candidate for the development of new antimalarial drugs.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Cysne DN,Fortes TS,Reis AS,de Paulo Ribeiro B,Dos Santos Ferreira A,do Amaral FM,Guerra RN,Marinho CR,Nicolete R,Nascimento FRdoi
10.1007/s00436-016-5216-xsubject
Has Abstractpub_date
2016-11-01 00:00:00pages
4327-4334issue
11eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5216-xjournal_volume
115pub_type
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