Abstract:
:The Stomacher is very widely used in food and medical research for extracting tissues. To determine whether nematode larvae were disrupted by the Stomacher, L3 larvae of Haemonchus contortus were homogenised for up to 40 min at full power but no larval disruption occurred. Therefore, tissue from the mucosa and submucosa of the caecum of horses collected from a licenced abattoir was treated to determine whether inhibited cyathostomin larvae could be extracted. The optimum time on full power for a 10-g sample was 20 min, and in three out of five caecal samples from different horses, significantly more larvae were recovered than with 6 h pepsin HCl digestion. It is concluded that the Stomacher provides a simple fast method of extracting inhibited nematode larvae from gastrointestinal tissues in the horse that could replace digestion with pepsin HCl.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Glover ID,Henry GM,Townsend NB,Coles GCdoi
10.1007/s00436-009-1473-2subject
Has Abstractpub_date
2009-08-01 00:00:00pages
587-9issue
2eissn
0932-0113issn
1432-1955journal_volume
105pub_type
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