Mechanical recovery of inhibited cyathostomin larvae from equine intestinal tissue.

Abstract:

:The Stomacher is very widely used in food and medical research for extracting tissues. To determine whether nematode larvae were disrupted by the Stomacher, L3 larvae of Haemonchus contortus were homogenised for up to 40 min at full power but no larval disruption occurred. Therefore, tissue from the mucosa and submucosa of the caecum of horses collected from a licenced abattoir was treated to determine whether inhibited cyathostomin larvae could be extracted. The optimum time on full power for a 10-g sample was 20 min, and in three out of five caecal samples from different horses, significantly more larvae were recovered than with 6 h pepsin HCl digestion. It is concluded that the Stomacher provides a simple fast method of extracting inhibited nematode larvae from gastrointestinal tissues in the horse that could replace digestion with pepsin HCl.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Glover ID,Henry GM,Townsend NB,Coles GC

doi

10.1007/s00436-009-1473-2

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

587-9

issue

2

eissn

0932-0113

issn

1432-1955

journal_volume

105

pub_type

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