Abstract:
:The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 expressed sequence tags were grouped into 1,212 clusters representing a nonredundant catalog of H. schachtii genes. Implementing a bioinformatic workflow, we identified 50 sequences coding for candidate secreted proteins. All of these contain a putative signal peptide required for entry into the secretory pathway and lack any transmembrane domain. Included are previously postulated cell-wall-degrading enzymes and other parasitism-related genes. Moreover, we provide the first report of an arabinogalactan endo-1,4-beta-galactosidase enzyme (EC 3.2.1.89) in animals. As sequence data increase at a rapid rate, developing high-throughput genomic screening is a necessity. The in silico approach described here is an effective way to identify putative secreted proteins and prioritize candidates for further studies.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Vanholme B,Mitreva M,Van Criekinge W,Logghe M,Bird D,McCarter JP,Gheysen Gdoi
10.1007/s00436-005-0029-3subject
Has Abstractpub_date
2006-04-01 00:00:00pages
414-24issue
5eissn
0932-0113issn
1432-1955journal_volume
98pub_type
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