Abstract：
:We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27 degrees C, and does not require co-culture with bacteria or any special equipment.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Axelsson-Olsson D,Olofsson J,Ellström P,Waldenström J,Olsen Bdoi
10.1007/s00436-008-1304-xsubject
Has Abstractpub_date
2009-03-01 00:00:00pages
935-7issue
4eissn
0932-0113issn
1432-1955journal_volume
104pub_type
杂志文章abstract：:The experimental transmission of a recently identified new Theileria (China 1) species pathogenic for sheep and goats in northern China is described. Haemaphysalis qinghaiensis nymphs and adults developed from larvae and nymph engorged on sheep infected with Theileria sp. (China 1) were able to respectively transmit t...
journal_title：Parasitology research
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