Combination of cell culture and qPCR to assess the efficacy of different anticoccidials on Eimeria tenella sporozoites.


:Three in vitro studies were designed to develop an assay for anticoccidial efficacy by use of laboratory (Houghton) and field (T-376) Eimeria tenella strains. In study (1), minimum inhibitory concentrations (MICs) of monensin (Mon), maduramicin (Mad), salinomycin (Sal), and lasalocid (Las) were determined that are able to inhibit more than 50% of sporozoites in host cell (Madin-Darby bovine kidney (MDBK)) penetration and more than 95% of Houghton sporozoites development to mature merozoites (treatment time 24 h) using quantitative real-time PCR (qPCR). MICs were 0.5, 2.5, 1, and 0.5 μg/ml for Mon, Mad, Sal, and Las, respectively. Applying the previous MIC on T-376 strain revealed a different sensitivity profile. Mad reduced T-376 gene copies by only 89.3% after 96 h of infection. In study (2), Houghton strain sporozoites were incubated with MIC of the different tested ionophores for 2 and 4 h, respectively; afterwards, their ability to invade MDBK cells was determined using phase-contrast microscopy and qPCR. Treatment of sporozoites with ionophores for 4 h resulted in significant inhibition of invasion compared with non-treated parasites as assessed both by microscopy as well as qPCR. Inhibition rates for Mon, Mad, Sal, and Las were 90.2, 75.0, 88.3, and 82.6% using phase-contrast microscopy and 83.9, 81.4, 85.8, and 75.4% using qPCR, respectively. T-376 sporozoite invasion into MDBK cells was reduced to 48.9% by Mad. Study (3) was conducted to determine inhibition exerted by toltrazuril (Tol). Tol at 5 μg/ml reduced reproduction of Houghton strain by 95%, whereas T-376 was only reduced by 86.5%. The presented experiments indicate that infectivity inhibition of sporozoites incubated for 4 h with anticoccidials and development inhibition after 96 h of infection by qPCR are suitable means to assess sensitivity of E. tenella strains to anticoccidials.


Parasitol Res


Parasitology research


Thabet A,Alnassan AA,Daugschies A,Bangoura B




Has Abstract


2015-06-01 00:00:00












  • The effects of interleukin (IL)-12 and IL-4 deficiency on worm development and granuloma formation in Schistosoma japonicum-infected mice.

    abstract::CD4(+) T-helper (Th) cell is widely recognized to be capable of influencing worm development and egg granuloma formation after schistosome infection. Interleukin (IL)-12 and IL-4 play key roles in regulation of Th cell differentiation. In the present study, we subcutaneously inoculated mice with hybridoma cells secret...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Cheng YL,Song WJ,Liu WQ,Lei JH,Kong Z,Li YL

    更新日期:2012-01-01 00:00:00

  • Surprisingly long body length of the lungworm Parafilaroides gymnurus from common seals of the Dutch North Sea.

    abstract::Lungworms of the genera Parafilaroides and Otostrongylus are responsible for parasitic bronchopneumonia, the foremost disease of eastern Atlantic common seals (EACS, Phoca vitulina vitulina) in the Dutch North Sea. Recently, there have been increased reports of lungworm cases and observations of unusually long Parafil...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Elson-Riggins JG,Gibbons LM,Van Liere DW,Zinkstok EW,Blake DP,Alegre F,Spittle H,Brakefield PM,Udo de Haes HA,Osinga N

    更新日期:2020-06-01 00:00:00

  • Immune responses of the domestic fowl to Dermanyssus gallinae under laboratory conditions.

    abstract::There appear to be few reports in the literature regarding the host-poultry red mite (Dermanyssus gallinae) immunological relationship, despite the negative impact D. gallinae can have on both bird welfare and egg production, as well as its potential to act as a reservoir of zoonotic infections. The current study inve...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Harrington DW,Robinson K,Sparagano OA

    更新日期:2010-05-01 00:00:00

  • Meta-analysis of the diagnostic efficiency of the questionnaires screening for schistosomiasis.

    abstract::Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagn...

    journal_title:Parasitology research

    pub_type: 杂志文章,meta分析


    authors: Yang F,Tan XD,Liu B,Yang C,Ni ZL,Gao XD,Wang Y

    更新日期:2015-09-01 00:00:00

  • Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry.

    abstract::Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed t...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Melo CF,Esteves CZ,de Oliveira RN,Guerreiro TM,de Oliveira DN,Lima EO,Miné JC,Allegretti SM,Catharino RR

    更新日期:2016-11-01 00:00:00

  • Ancient parasites from endemic deer from "CUEVA PARQUE DIANA" archeological site, Patagonia, Argentina.

    abstract::The narrow Andean-Patagonian temperate rainforest strip in the west of southern South America is inhabited by two endemic species of cervids, the southern pudu (Pudu puda) and the huemul (Hippocamelus bisulcus), both cataloged as near threatened and threatened species, respectively. One of the possible causes of their...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Beltrame MO,Tietze E,Pérez AE,Bellusci A,Sardella NH

    更新日期:2017-05-01 00:00:00

  • Development of a loop-mediated isothermal amplification (LAMP) and a direct LAMP for the specific detection of Nosema ceranae, a parasite of honey bees.

    abstract::Nosema ceranae is a ubiquitous microsporidian pathogen infecting the midgut of honey bees. The infection causes bee nosemosis, a disease associated with malnutrition, dysentery, and lethargic behavior, and results in considerable economic losses in apiculture. The use of a rapid, sensitive, and inexpensive DNA-based m...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Lannutti L,Mira A,Basualdo M,Rodriguez G,Erler S,Silva V,Gisder S,Genersch E,Florin-Christensen M,Schnittger L

    更新日期:2020-12-01 00:00:00

  • Characterization of Myxidium spinibarba sp. nov. (Cnidaria, Myxosporea, Myxidiidae) from Spinibarbus sinensis (Bleeker, 1871) in Chongqing China.

    abstract::In the present study, we described a new species of Myxidium Bütschli, 1882, obtained from the gallbladder of Spinibarbus sinensis (Bleeker, 1871) from the Jialing River in Chongqing, China. Myxidium spinibarba sp. nov. was identified based on morphological and SSU rDNA sequence data. The mature myxospores were fusifo...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Chen W,Yang C,Zhao Y

    更新日期:2020-05-01 00:00:00

  • Trypanosoma cruzi: experimental parasitism of bone and cartilage.

    abstract::Trypanosoma cruzi causes Chagas' disease, a systemic infection that affects cells of meso-, endo-, and ectodermic origin. However, as far as we know, the presence of T. cruzi stages in bone has not been reported previously, and it has scarcely been investigated in cartilage. We inoculated 7- and 20-day-old (8 and 15 g...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Morocoima A,Rodríguez M,Herrera L,Urdaneta-Morales S

    更新日期:2006-11-01 00:00:00

  • Linking specialisation to diversification in the Diplectanidae Bychowsky 1957 (Monogenea, Platyhelminthes).

    abstract::The hypothesis of a positive correlation between host specificity and taxonomic diversification was tested in a family of fish ectoparasites, the Diplectanidae Bychowsky 1957 (Monogenea). A comparative analysis of correlation of species richness with host specificity was performed using an adapted independent contrast...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Desdevises Y,Morand S,Oliver G

    更新日期:2001-03-01 00:00:00

  • Inheritance of pyrethroid resistance and a sodium channel gene mutation in the cattle tick Boophilus microplus.

    abstract::A substitution (Phe-->Ile) within the sodium channel gene sequence has been associated with pyrethroid resistance in Boophilus microplus. The aim of the present study was to analyze the inheritance of pyrethroid resistance and the mutant allele, on reciprocal crosses of a susceptible (SS) and a resistant (RR) strain. ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Aguilar-Tipacamú G,Miller RJ,Hernández-Ortiz R,Rodriguez-Vivas RI,Vásquez-Peláez C,García-Vázquez Z,Olvera-Valencia F,Rosario-Cruz R

    更新日期:2008-08-01 00:00:00

  • The sensitivity of artesunate against Schistosoma japonicum decreased after 10 years of use in China?

    abstract::Artesuante (AS) is a good chemoprophylactic drug for preventing against schistosome infection, but Hua et al. recently reported that the sensitivity of AS against Schistosoma japonicum decreased after 10 years of use in China. There are at least three problems, to our knowledge, making the finding suspicious or inconc...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu R,Dong HF,Jiang MS

    更新日期:2012-04-01 00:00:00

  • Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings.

    abstract::Radiological features and biochemical changes were investigated during the parenchymal and ductal phases of chronic Fasciola hepatica infection in sheep. The activities of plasma aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and gamma-glutamyl transpeptidase (GGT), plasma levels of IgG anti- F. hepatic...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Gonzalo-Orden M,Millán L,Alvarez M,Sánchez-Campos S,Jiménez R,González-Gallego J,Tuñón MJ

    更新日期:2003-08-01 00:00:00

  • Identification and expression of maebl, an erythrocyte-binding gene, in Plasmodium gallinaceum.

    abstract::Avian malaria is of significant ecological importance and serves as a model system to study broad patterns of host switching and host specificity. The erythrocyte invasion mechanism of the malaria parasite Plasmodium is mediated, in large part, by proteins of the erythrocyte-binding-like (ebl) family of genes. However...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Martinez C,Marzec T,Smith CD,Tell LA,Sehgal RN

    更新日期:2013-03-01 00:00:00

  • Mycophenolic acid induces differentiation of Toxoplasma gondii RH strain tachyzoites into bradyzoites and formation of cyst-like structure in vitro.

    abstract::The biochemical and structural changes that occur during the conversion of Toxoplasma gondii tachyzoites to bradyzoites and the formation of tissue cyst are not well understood. Maintaining cells infected with T. gondii type II and III strains under stress conditions induces the tachyzoite-bradyzoite in vitro differen...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Castro-Elizalde KN,Hernández-Contreras P,Ramírez-Flores CJ,González-Pozos S,Gómez de León CT,Mondragón-Castelán M,Mondragón-Flores R

    更新日期:2018-02-01 00:00:00

  • The effect of dimethylsulfoxide on the tegumental brush border of the cestode Hymenolepis diminuta.

    abstract::Dimethyl sulfoxide (DMSO), commonly used for cryoprotection or for the solvation of cytoskeleton-modifying drugs, causes changes in the topology of the plasma membrane of the tegumental brush border in the tapeworm Hymenolepis diminuta; however, relatively long exposures of high concentrations are required. In tapewor...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Forman LA,Oaks JA

    更新日期:1992-01-01 00:00:00

  • Development of a highly sensitive IgG-ELISA based on recombinant arginine kinase of Toxocara canis for serodiagnosis of visceral larva migrans in the murine model.

    abstract::Toxocariasis is a worldwide zoonotic disease caused by the ascarid nematode Toxocara canis. The most common method available for serodiagnosis of toxocariasis is an enzyme-linked immunosorbent assay (ELISA) test using Toxocara excretory-secretory antigen (TES). The present study describes the development of IgG-ELISA ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wickramasinghe S,Yatawara L,Nagataki M,Takamoto M,Watanabe Y,Rajapakse RP,Uda K,Suzuki T,Agatsuma T

    更新日期:2008-09-01 00:00:00

  • Prevalence, intensity and seasonality of gastrointestinal parasites in abattoir horses in Germany.

    abstract::Prevalence and intensity of gastrointestinal parasites were studied through a longitudinal survey in 400 horses over a 17-month period in an abattoir in Germany. Three hundred and ten horses (77.5 %) were demonstrated harbouring endoparasites either by direct recovery of parasites from the digestive tract and/or in te...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Rehbein S,Visser M,Winter R

    更新日期:2013-01-01 00:00:00

  • Transcription status of vaccine candidate genes of Plasmodium falciparum during the hepatic phase of its life cycle.

    abstract::The CSP, EMP2/MESA, MSP2, MSP3, MSP5, RAP1, RAP2, RESA1, SERA1 and SSP2/TRAP genes of Plasmodium falciparum are vaccine candidates. The hepatic phase of the infection is of major interest due to the protection induced by immunization with radiation-attenuated sporozoites. We therefore performed RT-PCR experiments to d...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bodescot M,Silvie O,Siau A,Refour P,Pino P,Franetich JF,Hannoun L,Sauerwein R,Mazier D

    更新日期:2004-04-01 00:00:00

  • The common frog (Rana temporaria) as a potential paratenic and intermediate host for Angiostrongylus vasorum.

    abstract::Common frogs (Rana temporaria) were exposed either to third-stage larvae (L3) or to first-stage larvae (L1) of Angiostrongylus vasorum. Following exposure to L3, viable larvae could be detected in the frogs for at least 2 weeks. Following exposure to L1, the frogs developed viable L3 in their tissues within 30 days. I...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bolt G,Monrad J,Frandsen F,Henriksen P,Dietz HH

    更新日期:1993-01-01 00:00:00

  • Borrelia burgdorferi infection prevalences in questing Ixodes ricinus ticks (Acari: Ixodidae) in urban and suburban Bonn, western Germany.

    abstract::From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket dragging at 45 sites all over the city area of Bonn, western Germany, to be checked for Borrelia burgdorferi infection. The collection sites included 20 private gardens, nine ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Maetzel D,Maier WA,Kampen H

    更新日期:2005-01-01 00:00:00

  • Genetic diversity of an avian nasal schistosome causing cercarial dermatitis in the Black Sea-Mediterranean migratory route.

    abstract::This study is part of an effort to document the diversity of avian schistosomes in ducks and snails in Northern Iran, a major flyway (Black Sea/Mediterranean) for migratory birds and where cercarial dermatitis (CD) is prevalent in rice growing areas. CD is an allergic skin reaction from schistosome trematodes that eme...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ashrafi K,Nouroosta A,Sharifdini M,Mahmoudi MR,Rahmati B,Brant SV

    更新日期:2018-12-01 00:00:00

  • Annulotrema (Monogenea: Dactylogyridae) from the gills of African tetras (Characiformes: Alestidae) in Lake Turkana, Kenya, with descriptions of four new species and a redescription of A. elongata Paperna and Thurston, 1969.

    abstract::Four new and four previously described species of Annulotrema were collected from the gills of four species (three genera, i.e. Alestes, Hydrocynus and Brycinus) of African tetras from Lake Turkana, Kenya: Annulotrema alestesnursi Paperna, 1973 from Brycinus nurse; Annulotrema ansatum n. sp., Annulotrema besalis Řehul...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kičinjaová ML,Blažek R,Gelnar M,Řehulková E

    更新日期:2015-11-01 00:00:00

  • Adulticide effect of Monticalia greenmaniana (Asteraceae) against Lutzomyia migonei (Diptera: Psychodidae).

    abstract::Leishmaniasis is a public health problem that has been increasing year by year, with the further difficulty that an efficient control system is not available. Therefore, it is necessary to search for less contaminating and dangerous alternatives for controlling Leishmania transmitting sandflies. The purpose of this st...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Cárdenas J,Rojas J,Rondón M,Nieves E

    更新日期:2012-08-01 00:00:00

  • Factors affecting the component community structure of haemoparasites in common voles ( Microtus arvalis) from the Mazury Lake District region of Poland.

    abstract::The prevalence and abundance of infections with haemoparasites were studied over a 4-year period in Microtus arvalis (common vole, n =321) sampled from fallow grassland sites in north-eastern Poland. Total species richness was five (prevalence= Haemobartonella sp. 63.9%, Bartonella spp. 27.7%, Babesia microti 9.0%, Tr...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Pawelczyk A,Bajer A,Behnke JM,Gilbert FS,Sinski E

    更新日期:2004-03-01 00:00:00

  • Proteomic survey of the cestode Mesocestoides corti during the first 24 hours of strobilar development.

    abstract::Despite the fact that cestodes represent major etiological agents of both human and domestic animal diseases, little is known about the molecular aspects of cestode development. In this work, Mesocestoides corti, a model cestode species, was studied from the early development of its larval form (tetrathyridium) into a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Laschuk A,Monteiro KM,Vidal NM,Pinto PM,Duran R,Cerveñanski C,Zaha A,Ferreira HB

    更新日期:2011-03-01 00:00:00

  • Toxoplasma gondii infection in the peritoneal macrophages of rats treated with glucocorticoids.

    abstract::It is well known that toxoplasmosis can be life threatening to immunocompromised individuals such as AIDS and organ transplantation patients. Glucocorticoids (GCs) are widely used in the clinic for the treatment of autoimmune diseases and organ transplantation resulting in acute toxoplasmosis in these patients. Howeve...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wang T,Gao JM,Yi SQ,Geng GQ,Gao XJ,Shen JL,Lu FL,Wen YZ,Hide G,Lun ZR

    更新日期:2014-01-01 00:00:00

  • In vitro uptake and autoradiographic localization of tritiated gossypol in Taenia taeniaeformis metacestodes.

    abstract::Gossypol, a natural polyphenolic compound, induces growth-inhibitory and antiparasitic effects in Taenia taeniaeformis metacestodes in vivo and in vitro. We investigated the uptake and localization of [3H]-gossypol in this parasite. Metacestodes were incubated in 10(-5) M [3H]-gossypol at 37 degrees C. Parasites stead...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kulp SK,Rikihisa Y,Lin YC,Moh PP,Li PK,Gu Y

    更新日期:1993-01-01 00:00:00

  • Relationships between vector-borne parasites and free-living mammals at the Brazilian Pantanal.

    abstract::In nature, parasitic infections must be addressed as complex systems involving parasite-host relationships on a temporal and spatial scale. Since the parasites cover a great biological diversity, we can expect that wildlife are exposed simultaneously to different parasites. In this sense, the objective of this work wa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Santos FM,de Sousa KCM,Sano NY,Nantes WAG,Liberal SC,Machado RZ,André MR,Herrera HM

    更新日期:2021-01-09 00:00:00

  • In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus).

    abstract::In search of a natural antiparasitic, in vivo anthelmintic activity of petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts of Angelica pubescens roots (Radix angelicae pubescentis), Brucea javanica fruits (Fructus bruceae), Spatholobus suberectus stems (Caulis spatholobi), Aesculus chinensis Bge...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu YT,Wang F,Wang GX,Han J,Wang Y,Wang YH

    更新日期:2010-04-01 00:00:00