Abstract：
:A LightCycler real-time polymerase chain reaction (PCR) assay was developed to detect Wuchereria bancrofti DNA in blood-fed mosquitoes. The assay is based on fluorescence melting curve analysis of the PCR product generated from a family of repeated DNA elements: the 182 bp SspI repeat, specific to the genus Wuchereria. According to the melting temperature, W. bancrofti infected-mosquitoes were differentiated from Brugia malayi-infected and non-infected mosquitoes as well as from genomic DNA of Dirofilaria immitis and human DNA. The method proved to be 100% sensitive in all W. bancrofti-infected mosquitoes. Melting curve analysis offers a rapid alternative for the specific detection of W. bancrofti in mosquitoes. It is very accurate and sensitive, allows a high throughput and can be performed on very small samples. The method therefore has great potential for application in epidemiological studies.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Lulitanond V,Intapan PM,Pipitgool V,Choochote W,Maleewong Wdoi
10.1007/s00436-004-1221-6subject
Has Abstractpub_date
2004-11-01 00:00:00pages
337-41issue
5eissn
0932-0113issn
1432-1955journal_volume
94pub_type
杂志文章abstract：:Haemonchus contortus isolates were evaluated for benzimidazole (BZ) resistance or susceptibility by allele-specific PCR based on β-tubulin isotype 1 gene polymorphisms at the F167Y, E198A, and F200Y sites. Two isolates, one presumed susceptible from wild pronghorn antelope (PH) and one known to be resistant from goats...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5357-y
更新日期：2017-02-01 00:00:00
abstract：:Litomosoides chagasfilhoi is a filariid nematode parasite of the abdominal cavity of the wild rodent Akodon cursor (Winge, 1887), that has been described and used in Brazil as a new model for human filariasis. The fine structure of the intestine of this nematode was analyzed based on observations made by light and tra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0092-9
更新日期：2006-05-01 00:00:00
abstract：:The G-Protein-coupled receptor Hc110-R of Haemonchus contortus and its orthologue in Caenorhabditis elegans, the latrophilin-like protein 1 (LAT-1), were shown to play a role in the mode of action of the new anthelmintic compound emodepside. C. elegans LAT-1 knockout mutants showed a decreased paralysing effect of emo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1500-3
更新日期：2009-08-01 00:00:00
abstract：:The localisation and distribution of the cytoskeletal protein tubulin in the adult liver fluke Fasciola hepatica have been determined by an indirect immunofluorescence technique using a monoclonal antibody raised against beta-tubulin. Tubulin was demonstrated in the tegumental syncytium and in the tegumental cell bodi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931649
更新日期：1992-01-01 00:00:00
abstract：:Two neolignans, burchellin and nordihydroguaiaretic acid (NDGA), were toxic only to Trypanosoma cruzi clone Dm28c maintained in brain heart infusion (BHI) medium at a concentration of 100 microg/ml, not 10 microg/ml. When Rhodnius prolixus was fed with epimastigotes of T. cruzi and treated simultaneously with a single...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050532
更新日期：1999-03-01 00:00:00
abstract：:Argulus are common aquatic ectoparasites that create one of the major threats to aquaculture due to absence of suitable therapy. Piperine, a bioactive component of Piper longum, has medicinal properties and acts as anti-inflammatory, antibacterial, and antifungal, considering eco-friendliness and cost-effectiveness. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3054-z
更新日期：2012-11-01 00:00:00
abstract：:Understanding the fundamental factors influencing the epidemiology of wildlife disease is essential to determining the impact of disease on individual health and population dynamics. The host-pathogen-environment relationship of the endangered Australian sea lion (Neophoca cinerea) and the parasitic hookworm, Uncinari...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3997-3
更新日期：2014-09-01 00:00:00
abstract：:The malaria parasite, Plasmodium falciparum, invades the human erythrocyte through a complex interaction with erythrocyte receptors characterized by patterns of resistance to various enzymes. As invasion rates are influenced by blood group polymorphisms, we reasoned that the extremely rare rhesus null (Rh(null)) eryth...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0756-8
更新日期：2008-01-01 00:00:00
abstract：:The levels of parasite-specific IgG1 and IgG2 antibodies and mitogen-induced and parasite-specific proliferative T-cell responses were determined in Litomosoides sigmodontis-infected Mastomys coucha throughout an observation period of 400 days post infection (p.i.). These were compared with the respective reactions in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050018
更新日期：2000-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3044-1
更新日期：2012-11-01 00:00:00
abstract：:Cytokine-induced apoptosis inhibitor (CIAP) is a novel antiapoptotic molecule, which is different to inhibitor of apoptosis protein or B-cell lymphoma 2. CIAP was originally identified as a molecule that conferred resistance to apoptosis induced by growth factor starvation. However, it remains to be undercharacterized...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3086-4
更新日期：2012-12-01 00:00:00
abstract：:Malaria recurrences after an initially successful therapy and malarial fever occurring a long time after infection are well-known problems in malariology. Currently, two distinct types of malaria recurrences are defined: recrudescence and relapse. A recrudescence is thought to originate from circulating Plasmodium blo...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-016-5043-0
更新日期：2016-06-01 00:00:00
abstract：:Spermiogenesis and the ultrastructure of the spermatozoon of the bothriocephalidean cestode Clestobothrium crassiceps (Rudolphi, 1819), a parasite of the teleost fish Merluccius merluccius (Linnaeus, 1758), have been studied by means of transmission electron microscopy. Spermiogenesis involves firstly the formation of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2446-9
更新日期：2012-01-01 00:00:00
abstract：:Intestines of California sea lion (Zalophus californianus) pups (n= 204), born in 2002 on San Miguel Island, California, were examined for hookworms (Uncinaria spp.) as part of a seasonal mortality study from June through December 2002 and January 2003. The investigation was planned to coincide with most of the previo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1335-5
更新日期：2005-05-01 00:00:00
abstract：:For the identification of host species of blood-sucking parasites, the suitability of disc-electrophoresis of the stomach contents was tested. Mammalian blood in the stomach of the medicinal blood leech Hirudo medicinalis gave satisfactory results. In the case of mixed blood samples from H. medicinalis, the identifica...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931901
更新日期：1993-01-01 00:00:00
abstract：:Nanotechnology is a broad interdisciplinary area of research, development, and industrial activity that has been growing rapidly worldwide for the past decade. More ambitious uses of nanoparticles are bioremediation of contaminated environments, controlled release of fragrances, biocides, and antifungals on textiles. ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-008-0975-7
更新日期：2008-07-01 00:00:00
abstract：:Chagas' disease, which is an important health problem in humans, is caused by the protozoan Trypanosoma cruzi. The cellular and molecular mechanisms, involved in the selective tropism of T. cruzi to different organs remain largely unknown. In this study we designed a PCR-based molecular diagnosis method in order to st...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0787-0
更新日期：2003-04-01 00:00:00
abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Echinococcus coproantigens in fecal samples from dogs, dingoes or foxes infected with either E. granulosus or E. multilocularis. The ELISA was based on protein-A-purified polyclonal antibodies [anti-E. granulosus excretory/secretory (E/S) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00937088
更新日期：1992-01-01 00:00:00
abstract：:In life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0834-y
更新日期：2008-04-01 00:00:00
abstract：:Precise identification of members of the genus Diplostomum is difficult in that many larval stages have been described and named separately from their adult forms. In this study, the life cycles of two Diplostomum spp. were established using metacercariae obtained from the eyes of rainbow trout. The parasites were ide...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931794
更新日期：1995-01-01 00:00:00
abstract：:A study has been carried out to investigate whether the action of triclabendazole (TCBZ) against Fasciola hepatica is altered by inhibition of drug metabolism. The cytochrome P450 (CYP 450) enzyme pathway was inhibited using ketoconazole (KTZ) to see whether a TCBZ-resistant isolate could be made more sensitive to TCB...
journal_title：Parasitology research
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1007/s00436-011-2554-6
更新日期：2011-10-01 00:00:00
abstract：:Little is known of the prevalence and genetic identity of Giardia duodenalis in sheep in Algeria. The present study aimed at characterizing G. duodenalis in lambs up to 6 months of age in Djelfa, Algeria. A total of 346 fecal specimens were collected from 28 farms and screened for G. duodenalis cysts by zinc sulfate f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06808-y
更新日期：2020-09-01 00:00:00
abstract：:The efficacies of 20 mg/kg body weight (BW) of toltrazuril (Toltra) 2 days post-infection (dpi), 2 mg/kg BW of diclazuril 2 and 3 dpi and 200 mg/kg BW of sulphadimidine 2, 3 and 4 dpi were compared in a model for piglet isosporosis. Weight gain (first 4 weeks of life) and diarrhoea and oocyst excretion from 4 to 11 dp...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-006-0314-9
更新日期：2007-01-01 00:00:00
abstract：:Macroscopically visible sarcocysts were observed in the skeletal muscles of naturally infected skinks of the genus Agama (infection rate 11.3%). Sarcocysts were described by means of transmission electron microscopy. These cysts measured 0.03-0.25 x 0.38-1.7 mm (mean 0.12 x 1.1 mm). Typical mature cysts were bordered ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008559
更新日期：2000-09-01 00:00:00
abstract：:Galectin plays an important role in host-parasite interactions. In this study, we identified a novel gene encoding galectin-10 (AcGal-10) from the cDNA library of Angiostrongylus cantonensis and characterized its biological role in the parasite. Sequence and phylogeny analysis showed that AcGal-10 is related to other ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3210-5
更新日期：2013-03-01 00:00:00
abstract：:Leishmania (L.) tropica is a causative agent of cutaneous and occasionally visceral or viscerotropic leishmaniasis in humans. The dose of parasites influences the course and outcome of disease in some Leishmania species. The effect of parasite dose on L. tropica infection in an experimental model was studied in the cu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5991-7
更新日期：2018-09-01 00:00:00
abstract：:Free-living amoebae (FLA) are opportunistic and ubiquitous protozoa that are widely found in various environmental sources. They are known to cause serious human infections. The aim of our study was to detect FLA and Acanthamoeba spp. in hospital water circuits. Eighty-four water samples were collected over a period o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4788-1
更新日期：2016-01-01 00:00:00
abstract：:During a parasitological survey of two non-native fish species-racer goby Neogobius gymnotrachelus and monkey goby Neogobius fluviatilis-in the Włocławek Reservoir on the lower Vistula River, the monogenean Gyrodactylus proterorhini was recorded for the first time in Poland, and for the first time, the racer goby was ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2175-5
更新日期：2011-05-01 00:00:00
abstract：:The proboscis apex of the eoacanthocephalan parasite Paratenuisentis ambiguus was studied, using electron and light microscopy, for a better understanding of the parasite's attachment at the intestinal wall of its definitive hosts (Anguilla rostrata and A. anguilla). The results suggest the presence of an epidermis co...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008583
更新日期：2001-04-01 00:00:00
abstract：:A high-resolution melting (HRM) assay was applied to distinguish between Giardia duodenalis assemblages A and B from human and dog feces based on the triosephosphate isomerase gene (tpi). The genomic DNAs were selected from assemblages A (WB) and B (GS) as reference and plasmids were constructed. The reference plasmid...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3064-x
更新日期：2012-11-01 00:00:00
