Abstract：
:A high-resolution melting (HRM) assay was applied to distinguish between Giardia duodenalis assemblages A and B from human and dog feces based on the triosephosphate isomerase gene (tpi). The genomic DNAs were selected from assemblages A (WB) and B (GS) as reference and plasmids were constructed. The reference plasmids and genomic DNAs from 15 Giardia-positive samples were analyzed by HRM assay. This was followed by separate real-time PCR assays specific for assemblages A and B using EvaGreen (EG) to identify PCR products by melting-point analysis. Our results indicate that PCR with HRM in a one-step closed-tube method is a reliable diagnostic method for G. duodenalis zoonotic assemblage identification and more rapid than restriction length polymorphism analysis and direct sequence analysis, HRM is specific, sensitive, reproducible, and rapid. This study is the first use of EG dye for Giardia genotyping. This assay is a promising approach to determine the presence and genotype of Giardia based on a highly variable gene.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Zhang P,Liu Y,Alsarakibi M,Li J,Liu T,Li Y,Li Gdoi
10.1007/s00436-012-3064-xsubject
Has Abstractpub_date
2012-11-01 00:00:00pages
2157-63issue
5eissn
0932-0113issn
1432-1955journal_volume
111pub_type
杂志文章abstract：:Chronic Chagas' disease affects 10-30 % of patients infected with Trypanosoma cruzi, and it mainly manifests as cardiomyopathy. Important pathophysiological mechanisms involved in the cardiac lesions include activation of the endothelium and induced microvascular alterations. These processes involve the production of ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3444-x
更新日期：2013-07-01 00:00:00
abstract：:The physicochemical properties of four N-halomethylated and one non-halomethylated ammonium salts, with proven in vitro antileishmanial activity, were determined according to pharmaceutical standard procedures. The effectiveness and toxicity of these compounds were assessed in hamsters infected with Leishmania (Vianni...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5702-9
更新日期：2018-01-01 00:00:00
abstract：:A taste-enhancement process for cold-stored, raw shell-stock Crassostrea virginica oysters (i.e., application of table salt to shells) when externally contaminated with human enteropathogens intensified spillage of these enteropathogens to oyster' storage containers (77% compared to 27% for controls) but did not, howe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0664-y
更新日期：2007-11-01 00:00:00
abstract：:Water buffalo is important livestock in several countries in the Latin American and Caribbean regions. This buffalo species can be infected by tick-borne hemoparasites and remains a carrier of these pathogens which represent a risk of infection for more susceptible species like cattle. Therefore, studies on the epidem...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-06194-6
更新日期：2019-03-01 00:00:00
abstract：:NADH- or NADPH-oxidase activity was cytochemically detected at the ultrastructural level during the process of interaction between Trypanosoma cruzi and activated mouse peritoneal macrophages. The reaction product, indicative of enzyme activity, was found in the portion of the plasma membrane of the macrophages to whi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00578506
更新日期：1987-01-01 00:00:00
abstract：:The phylogenetic placement of gregarine parasites (Apicomplexa: Gregarinasina) within the Apicomplexa was derived by comparison of small-subunit ribosomal RNA gene sequences. Gregarine sequences were obtained from Gregarina niphandrodes Clopton, Percival, and Janovy, 1991, and Monocystis agilis Stein, 1848 (Eugregarin...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050655
更新日期：1999-11-01 00:00:00
abstract：:This study was conducted to identify genetic characteristics of Besnoitia spp. isolated from goat in Iran. Molecular analysis of two portions of nuclear ribosomal DNA (ITS1 and ITS2) was used for the genetic characterization of the Besnoitia species. Comparison of the sequencing data of the Iranian Besnoitia samples o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2107-4
更新日期：2011-03-01 00:00:00
abstract：:Spermiogenesis and spermatozoon ultrastructure of the caryophyllidean cestode Breviscolex orientalis Kulakovskaya, 1962, first member of the family Capingentidae studied, a parasite of cyprinid fish Abbottina rivularis, are described using transmission electron microscopy. Spermiogenesis in B. orientalis follows the T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2144-z
更新日期：2011-04-01 00:00:00
abstract：:The present study represents the first detailed description of the haptoral sclerites of Macrogyrodactylus clarii Gussev 1961. Light microscopy reveals outgrowths of the hamuli roots; two lateral spine-like extensions of the hood-like accessory sclerites; a ridged, fan-shaped distal end of an accessory sclerite; and t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06799-w
更新日期：2020-12-01 00:00:00
abstract：:The blowfly Lucilia sericata (Diptera: Calliphoridae) is a facultative ectoparasite that causes myiasis in both man and animals, leading to serious human health problems and economic losses in the livestock industry. Like other insects, olfaction of this species plays an important role in host location and is presumab...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2563-5
更新日期：2012-02-01 00:00:00
abstract：:Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050419
更新日期：1998-05-01 00:00:00
abstract：:The essential oil of Cananga odorata flowers was evaluated for oviposition-deterrent, ovicidal, insecticidal, and repellent activities toward three mosquito species: Aedes aegypti, Anopheles dirus, and Culex quinquefasciatus. Oviposition deterrence of the oil was evaluated on gravid females using oviposition deterrenc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4699-1
更新日期：2015-12-01 00:00:00
abstract：:The problems of resistant lines of Plasmodium falciparum are escalating. Twelve seaweeds species belong to five different families (Sargassaceae, Gracilariaceae, Hypneaceae, Corallinaceae and Halimedaceae) were collected from Mandapam coastal area, and the seaweeds extracts were tested for in vitro antiplasmodial acti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2219-x
更新日期：2011-07-01 00:00:00
abstract：:Tick proteins have been shown to be useful for the development of vaccines which reduce tick infestations. Potential tick protective antigens have been identified and characterized, in part, by use of RNA interference (RNAi). RNAi allows for analysis of gene function by characterizing the impact of loss of gene expres...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0132-0
更新日期：2006-07-01 00:00:00
abstract：:Under natural conditions, eukaryote cells may contain bacteria. Arthropods such as ticks, insects or mites are a group particularly favoured by the obligate intracellular bacteria. While arthropods are vectors for some of them, other bacteria inhabit invertebrate host cells having entered mutualistic interactions. Suc...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-006-0412-8
更新日期：2007-04-01 00:00:00
abstract：:Over a 4-year period, we analyzed 128 fecal samples from free-living elk (Alces alces L., 1758) to determine the prevalence of Eimeria infections and identify the species present. Two eimerian species were isolated including Eimeria alces and a morphotype resembling Eimeria catubrina. Overall, two samples from 128 sam...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3262-6
更新日期：2013-05-01 00:00:00
abstract：:Lungworms of the genera Parafilaroides and Otostrongylus are responsible for parasitic bronchopneumonia, the foremost disease of eastern Atlantic common seals (EACS, Phoca vitulina vitulina) in the Dutch North Sea. Recently, there have been increased reports of lungworm cases and observations of unusually long Parafil...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06675-7
更新日期：2020-06-01 00:00:00
abstract：:Schistosomiasis seriously affects human health in tropical regions. Its prevention is more important than treatment, raising the need for effective control methods. Recently, the role of nanomaterials in medical science has been growing. The present study aimed to evaluate the potential effects of silver (Ag) and gold...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6093-2
更新日期：2018-12-01 00:00:00
abstract：:The epidemiological importance of increasing reports worldwide on Cryptosporidium contamination of oysters remains unknown in relation to foodborne cryptosporidiosis. Thirty market-size oysters (Crassostrea virginica), collected from each of 53 commercial harvesting sites in Chesapeake Bay, MD, were quantitatively tes...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0261-5
更新日期：2007-01-01 00:00:00
abstract：:Thelohania montirivulorum sp. nov., a new species of microsporidian parasite, was found in a highland population of the Australian yabby, Cherax destructor. Data are presented on fine ultrastructure, developmental morphology and DNA sequence of the small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-0948-9
更新日期：2003-10-01 00:00:00
abstract：:The gypsy virus of Drosophila melanogaster is the first identified retrovirus of insects. Its infectious properties have been reported, and it is able to cause diverse mutations of the phenotype in the fruit fly. As a typical endogenous retrovirus, it is transmitted vertically as part of the host genome, but horizonta...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0249-1
更新日期：2007-01-01 00:00:00
abstract：:In the present study the behavior and pathogenicity of second-stage larvae of Toxocara canis were examined in different mouse strains with special emphasis on the major histocompatibility complex (MHC). Mice of the inbred strains BALB, C3H, C57BL, and DBA and the outbred strain NMRI were infected orally with 1000 seco...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932955
更新日期：1994-01-01 00:00:00
abstract：:The aim of this review is to present briefly background information on 27 tick-borne viruses ("tiboviruses") that have been detected in Europe, viz flaviviruses tick-borne encephalitis (TBEV), louping-ill (LIV), Tyuleniy (TYUV), and Meaban (MEAV); orthobunyaviruses Bahig (BAHV) and Matruh (MTRV); phleboviruses Grand A...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-012-2910-1
更新日期：2012-07-01 00:00:00
abstract：:Sarcocystis sarcocysts are common in muscles of herbivores but are rare in muscles of carnivores. Here, we report sarcocysts in the muscles of a gray wolf (Canis lupus) from Alaska, USA, for the first time. Sarcocysts extracted from the tongue of the wolf were up to 900 μm long and slender and appeared to have a relat...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5067-5
更新日期：2016-07-01 00:00:00
abstract：:Using molecular techniques, we documented the presence of Nosema ceranae in honeybees (Apis mellífera) from Argentina. Samples were collected from A. mellifera colonies in 38 districts of Buenos Aires province, Argentina. Molecular characterization was achieved with a multiplex PCR-based method, which allows parallel ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2566-2
更新日期：2012-02-01 00:00:00
abstract：:Necrophagous Sarcophagidae are among the insects most frequently reported from human corpses. The broad forensic application of flesh flies is restricted by the lack of reliable tools for species identification of larval stages and mass breeding of collected flesh fly larvae to the adult stage, and more recently DNA-b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4421-3
更新日期：2015-06-01 00:00:00
abstract：:In freshwater ecosystems, snails can significantly influence the competition between primary producers through grazing of periphyton. This activity can potentially be modified by trematodes, a large group of parasites which mostly use molluscs as the first intermediate host. Available studies, however, show contradict...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6052-y
更新日期：2018-11-01 00:00:00
abstract：:Myxobolus turpisrotundus Zhang, 2009, infects allogynogenetic gibel carp Carassius auratus gibelio (Bloch) and is always regarded as synonymous with Myxobolus rotundus Nemeczek, 1911, since its first report in goldfish Carassius auratus auratus (L.) in China in 1955. In this study, it was comprehensively examined by m...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1835-9
更新日期：2010-06-01 00:00:00
abstract：:Production of parasite-specific antibodies is an important component of immunity to blood stage malaria infection, as shown by several previous studies in rodent models. However, no study has addressed the induction of humoral immunity by different parasites in a genetically homogeneous host population. Here, levels o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0687-3
更新日期：2003-01-01 00:00:00
abstract：:Spermiogenesis and the ultrastructure of the spermatozoon of the bothriocephalidean cestode Clestobothrium crassiceps (Rudolphi, 1819), a parasite of the teleost fish Merluccius merluccius (Linnaeus, 1758), have been studied by means of transmission electron microscopy. Spermiogenesis involves firstly the formation of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2446-9
更新日期：2012-01-01 00:00:00
