The putative telomerase reverse transcriptase component of Leishmania amazonensis: gene cloning and characterization.

Abstract:

:The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence alignment demonstrated that the Leishmania telomerases show greater homology with each other than with the proteins of other kinetoplastids and eukaryotes. Characterization experiments indicated that the putative Leishmania telomerase gene was probably in single copy and located in the largest chromosomes. A single messenger ribonucleic acid transcript was found in promastigotes. Phylogenetic analysis suggested that Leishmania telomerase might represent a liaison between the oldest and the newest branches of telomerases.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Giardini MA,Lira CB,Conte FF,Camillo LR,de Siqueira Neto JL,Ramos CH,Cano MI

doi

10.1007/s00436-005-0036-4

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

447-54

issue

5

eissn

0932-0113

issn

1432-1955

journal_volume

98

pub_type

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