Abstract:
:The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence alignment demonstrated that the Leishmania telomerases show greater homology with each other than with the proteins of other kinetoplastids and eukaryotes. Characterization experiments indicated that the putative Leishmania telomerase gene was probably in single copy and located in the largest chromosomes. A single messenger ribonucleic acid transcript was found in promastigotes. Phylogenetic analysis suggested that Leishmania telomerase might represent a liaison between the oldest and the newest branches of telomerases.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Giardini MA,Lira CB,Conte FF,Camillo LR,de Siqueira Neto JL,Ramos CH,Cano MIdoi
10.1007/s00436-005-0036-4subject
Has Abstractpub_date
2006-04-01 00:00:00pages
447-54issue
5eissn
0932-0113issn
1432-1955journal_volume
98pub_type
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journal_title:Parasitology research
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