A fast and inexpensive method for isolation of high-quality RNA from eimerian oocysts.

Abstract:

:An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Johnston DA,Bratty J,Fernando MA

doi

10.1007/s004360050446

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

549-51

issue

7

eissn

0932-0113

issn

1432-1955

journal_volume

84

pub_type

杂志文章
  • Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.

    abstract::Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5372-7

    authors: Abdel-Shafi IR,Shoieb EY,Attia SS,Rubio JM,Ta-Tang TH,El-Badry AA

    更新日期:2017-03-01 00:00:00

  • Plasmodium helical interspersed subtelomeric family-an enigmatic piece of the Plasmodium biology puzzle.

    abstract::Plasmodium falciparum (Pf) refurbishes the infected erythrocytes by exporting a myriad of parasite proteins to the host cell. A novel exported protein family 'Plasmodium Helical Interspersed Subtelomeric' (PHIST) has gained attention for its significant roles in parasite biology. Here, we have collected and analysed a...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审

    doi:10.1007/s00436-019-06420-9

    authors: Kumar V,Behl A,Sharma R,Sharma A,Hora R

    更新日期:2019-10-01 00:00:00

  • Development of Angiostrongylus costaricensis Morera and Céspedes 1971 (Nematoda: Angiostrongylidae) larvae in the intermediate host Sarasinula marginata (Semper 1885) (Mollusca: Soleolifera).

    abstract::In life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0834-y

    authors: Mendonça CL,Carvalho OS,Mota EM,Lenzi HL

    更新日期:2008-04-01 00:00:00

  • Electron microscopy studies on the a-bacteroids in the fat bodies of the lantern bug Pyrops candelaria Linn. (Homoptera: Fulgoridae).

    abstract::The ultrastructure of alpha-bacteroids in relation to the fat-body cells of the lantern bug Pyrops candelaria was described. The fat-body-cell cytoplasm contained numerous mitochondria, rough endoplasmic reticula, vacuoles, and storage granules. Its nucleus had scattered chromatin materials. The alpha-bacteroid was en...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050287

    authors: Wang JB,Cheung WW

    更新日期:1997-01-01 00:00:00

  • Giardia duodenalis: protein substrates degradation by trophozoite proteases.

    abstract::The present investigation was undertaken to identify and characterize trophozoite proteases of five axenic strains of Giardia duodenalis isolated in Brazil and the reference strain Portland 1 isolated in the United States. Trophozoite cell lysates of each strain were analysed for the pattern of proteins and for proteo...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-0124-5

    authors: Coradi ST,Guimarães S

    更新日期:2006-07-01 00:00:00

  • First record of anisakid juveniles (Nematoda) in the European seabass Dicentrarchus labrax (family: Moronidae), and their role as bio-indicators of heavy metal pollution.

    abstract::This study assessed the anisakid nematode distribution pattern in the fish collected from coasts of Mediterranean Sea, Egypt, during the period September 2010-April 2011. Two hundred thirty out of 300 (76.7%) Dicentrarchus labrax (European seabass) marine fishes belonging to family Moronidae were dissected and found t...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2600-4

    authors: Morsy K,Bashtar AR,Abdel-Ghaffar F,Mehlhorn H,Quraishy SA,El-Mahdi M,Al-Ghamdi A,Mostafa N

    更新日期:2012-03-01 00:00:00

  • Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats.

    abstract::The performance of a rapid immunochromatographic test for the detection of Dirofilaria immitis antigens (Speed Diro™; BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro™ showed a sensitivity of 90...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5709-2

    authors: Genchi M,Mangia C,Ferrari N,Loukeri S

    更新日期:2018-01-01 00:00:00

  • Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources.

    abstract::A comprehensive survey to document the presence of free-living amoebae of the genus Acanthamoeba was conducted in tap water and sea water sources related to human environments in Tenerife, Canary Islands, Spain. Acanthamoeba identification was based on the morphology of cyst and trophozoite forms and PCR amplification...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-1301-2

    authors: Lorenzo-Morales J,Ortega-Rivas A,Foronda P,Martínez E,Valladares B

    更新日期:2005-03-01 00:00:00

  • Detection of Cryptosporidium spp. from human faeces by PCR-RFLP, cloning and sequencing.

    abstract::In this study, we compared and validated a nested ABC polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay to amplify Cryptosporidium parvum oocyst wall protein (COWP) gene fragment with a previous nested PCR-RFLP method. When RFLP of COWP gene did not provide clear results, we further a...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-1233-8

    authors: Leone A,Ripabelli G,Sammarco ML,Grasso GM

    更新日期:2009-02-01 00:00:00

  • Intraspecific and interspecific genetic variation of Gongylonema pulchrum and two rodent Gongylonema spp. (G. aegypti and G. neoplasticum), with the proposal of G. nepalensis n. sp. for the isolate in water buffaloes from Nepal.

    abstract::The gullet worm (Gongylonema pulchrum) has been recorded from a variety of mammals worldwide. In an earlier study, we demonstrated two separate transmission cycles in cattle (Bos taurus) and wild mammals in Japan based on nucleotide sequences of the ribosomal RNA gene (rDNA) and cytochrome c oxidase subunit I (cox-1) ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4806-3

    authors: Setsuda A,Da N,Hasegawa H,Behnke JM,Rana HB,Dhakal IP,Sato H

    更新日期:2016-02-01 00:00:00

  • Biological nature of Cryptosporidium sp. isolated from a cat.

    abstract::A small type of Cryptosporidium oocysts was isolated from a naturally infected cat and its biological nature was investigated. In cats experimentally inoculated with Cryptosporidium oocysts, long-lasting shedding of the oocysts occurred after a prepatent period of 8-10 days, and a number of peaks of oocyst count appea...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00930864

    authors: Asahi H,Koyama T,Arai H,Funakoshi Y,Yamaura H,Shirasaka R,Okutomi K

    更新日期:1991-01-01 00:00:00

  • Molecular characterisation of Sarcocystis lutrae n. sp. and Toxoplasma gondii from the musculature of two Eurasian otters (Lutra lutra) in Norway.

    abstract::Sarcocysts were detected in routinely processed histological sections of skeletal muscle, but not cardiac muscle, of two adult male otters (Lutra lutra; Mustelidae) from northern Norway following their post-mortem examination in 1999 and 2000. The sarcocysts were slender, spindle-shaped, up to 970 μm long and 35-70 μm...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-4251-8

    authors: Gjerde B,Josefsen TD

    更新日期:2015-03-01 00:00:00

  • Characterization and bioassay for larvicidal activity of Anacardium occidentale (cashew) shell waste fractions against dengue vector Aedes aegypti.

    abstract::Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of A...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4598-5

    authors: Torres RC,Garbo AG,Walde RZ

    更新日期:2015-10-01 00:00:00

  • Characteristics of naturally acquired avian malaria infections in naive juvenile African black-footed penguins (Spheniscus demersus).

    abstract::Antibody responses to naturally acquired Plasmodium relictum and P. elongatum infections, blood parasitemia, and disease signs were investigated in 23 naive juvenile African black-footed penguins (Spheniscus demersus). Anti-Plasmodium spp. immunoglobulins were detected by enzyme-linked immunosorbent assay (ELISA) usin...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00932944

    authors: Graczyk TK,Cranfield MR,McCutchan TF,Bicknese EJ

    更新日期:1994-01-01 00:00:00

  • Eimeria vermiformis infection reduces goblet cells by multiplication in the crypt cells of the small intestine of C57BL/6 mice.

    abstract::In the gastrointestinal mucosa, mucus produced by goblet cells plays an important role in the defense against various pathogens. It is well known that some helminth parasites are able to up-regulate goblet cell numbers and alter the mucus components. However, the nature of the interactions between the protozoan parasi...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-1256-1

    authors: Linh BK,Hayashi T,Horii Y

    更新日期:2009-03-01 00:00:00

  • Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii.

    abstract::The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 e...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-0029-3

    authors: Vanholme B,Mitreva M,Van Criekinge W,Logghe M,Bird D,McCarter JP,Gheysen G

    更新日期:2006-04-01 00:00:00

  • Experimental transmission of Theileria sp. (China 1) infective for small ruminants by Haemaphysalis longicornis and Haemaphysalis qinghaiensis.

    abstract::The experimental transmission of a recently identified new Theileria (China 1) species pathogenic for sheep and goats in northern China is described. Haemaphysalis qinghaiensis nymphs and adults developed from larvae and nymph engorged on sheep infected with Theileria sp. (China 1) were able to respectively transmit t...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0509-8

    authors: Li Y,Luo J,Liu Z,Guan G,Gao J,Ma M,Dang Z,Liu A,Ren Q,Lu B,Liu J,Zhao H,Li J,Liu G,Bai Q,Yin H

    更新日期:2007-08-01 00:00:00

  • Ligictaluridus michaelalicea n. sp. (Monogenea: Dactylogyridae) from flathead catfish (Pylodictis olivaris) in the upper Mississippi River, including remarks on taxonomy influencing monogenean treatment regulation in the United States.

    abstract::Ligictaluridus michaelalicea n. sp. (Monogenea: Dactylogyridae, Ancyrocephalinae) is described from the gills of Pylodictis olivaris (Siluriformes: Ictaluridae) from Wisconsin and Iowa portions of the upper Mississippi River. Diagnostic features include a relatively large, strongly curved tubular cirrus with minor ter...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-018-5757-2

    authors: Leis E,Easy R,MacLean L,Cone D

    更新日期:2018-03-01 00:00:00

  • Lipoptena cervi (Diptera), a potential vector of Megatrypanum trypanosomes of deer (Cervidae).

    abstract::In three different areas in northern Germany, hippoboscids were collected from red deer (Cervus elaphus). Typanosomatids were demonstrated in the midgut and hindgut of 9/37 Lipoptena cervi. The trypanosomatids were morphologically similar to vector forms of the subgenus Megatrypanum that had been found in tabanids at ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00928691

    authors: Böse R,Petersen K

    更新日期:1991-01-01 00:00:00

  • The guild as a concept and a means in ecological parasitology.

    abstract::The use of the term guild community as an intermediate level between component and supra-community proves to be advantageous within the different steps of parasite communities. It can comprise more information than a component community but researchers are able to analyse important parts of the supra-community without...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审

    doi:10.1007/s004360100386

    authors: Zander CD

    更新日期:2001-06-01 00:00:00

  • Eosinophil chemotactic chemokine profilings of the brain from permissive and non-permissive hosts infected with Angiostrongylus cantonenis.

    abstract::Angiostrongylus cantonensis invasion primarily cause heavy or negligible eosinophic meningitis and meningoencephalitis in the brain of non-permissive and permissive hosts, respectively. Chemokines are effective leukocyte chemoattractants and may play an essential role in mediating eosinophil recruitment in angiostrong...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3683-x

    authors: Li S,Yang F,Ji P,Zeng X,Wu X,Wei J,Ouyang L,Liang J,Zheng H,Wu Z,Lv Z

    更新日期:2014-02-01 00:00:00

  • Meta-analysis of the diagnostic efficiency of the questionnaires screening for schistosomiasis.

    abstract::Schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. Questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. This meta-analysis was conducted to assess their diagn...

    journal_title:Parasitology research

    pub_type: 杂志文章,meta分析

    doi:10.1007/s00436-015-4579-8

    authors: Yang F,Tan XD,Liu B,Yang C,Ni ZL,Gao XD,Wang Y

    更新日期:2015-09-01 00:00:00

  • Geographical distribution of ticks in Turkey.

    abstract::Turkey's tick fauna is composed of about 32 species in two families and ten genera in mammals, reptiles, and birds. The ticks of veterinary significance in the family Ixodidae comprise seven genera with 28 different species. Ixodes spp. are mostly seen in northern Turkey. It is likely that the high rainfall and the in...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0694-5

    authors: Aydin L,Bakirci S

    更新日期:2007-09-01 00:00:00

  • Predominance of Blastocystis sp. subtype 4 in rural communities, Nepal.

    abstract::Blastocystis sp. is a common intestinal parasite. To date, there have been sporadic and scanty studies on Blastocystis sp. carried out in rural communities in Nepal. We surveyed the prevalence of Blastocystis sp. and its possible associated risk factors, and reported the predominant Blastocystis sp. subtype in two rur...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2665-0

    authors: Lee IL,Tan TC,Tan PC,Nanthiney DR,Biraj MK,Surendra KM,Suresh KG

    更新日期:2012-04-01 00:00:00

  • Pharmacokinetics and anthelmintic efficacy of topical eprinomectin in goats prevented from grooming.

    abstract::Pharmacokinetics and anthelmintic activity of topical eprinomectin in goats prevented from physical contact to others and self-grooming were studied. Sixteen approximately 7 months old male castrated German White Noble goats harbouring induced infections of gastrointestinal nematode parasites were included in the stud...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-4072-9

    authors: Rehbein S,Kellermann M,Wehner TA

    更新日期:2014-11-01 00:00:00

  • Novel Acanthamoeba 18S rRNA gene sequence type from an environmental isolate.

    abstract::The free-living amoebae, Acanthamoeba, can act as opportunistic parasites on a wide range of vertebrates and are becoming a serious threat to human health due to the resistance of their cysts to harsh environmental conditions, disinfectants, some water treatment practices, and their ubiquitous distribution. Subgenus c...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-3945-2

    authors: Magnet A,Henriques-Gil N,Galván-Diaz AL,Izquiedo F,Fenoy S,del Aguila C

    更新日期:2014-08-01 00:00:00

  • Compared vectorial transmissibility of pure and mixed clonal genotypes of Trypanosoma cruzi in Triatoma infestans.

    abstract::A total of 15 mixtures involving 9 different stocks attributed to the 19/20, 32 and 39 major clonal genotypes of Trypanosoma cruzi were used to infect third-instar nymphs of Triatoma infestans via an artificial feeding device. Three biological parameters were considered: (1) the percentage of infected insects (%II), (...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050409

    authors: Pinto AS,de Lana M,Bastrenta B,Barnabé C,Quesney V,Noël S,Tibayrenc M

    更新日期:1998-05-01 00:00:00

  • Thecamoeba quadrilineata (Amoebozoa, Lobosa) as a new member of amphizoic amoebae-first isolation from endozoic conditions.

    abstract::A free-living soil amoeba Thecamoeba quadrilineata (Carter, 1856) Lepşi, 1960 (Amoebozoa: Thecamoebidae) was isolated from endozoic conditions for the first time. Presence of amoebae was detected after 4 days following inoculation of the gut of the earthworm Lumbricus terrestris on agar plate with Escherichia coli. On...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-019-06207-y

    authors: Borovičková T,Mrva M,Garajová M

    更新日期:2019-03-01 00:00:00

  • Nanosilica-from medicine to pest control.

    abstract::Nanotechnology is a broad interdisciplinary area of research, development, and industrial activity that has been growing rapidly worldwide for the past decade. More ambitious uses of nanoparticles are bioremediation of contaminated environments, controlled release of fragrances, biocides, and antifungals on textiles. ...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审

    doi:10.1007/s00436-008-0975-7

    authors: Barik TK,Sahu B,Swain V

    更新日期:2008-07-01 00:00:00

  • Supplemental description and molecular characterization of Myxobolus miyarii Kudo, 1919 (Myxosporea: Myxobolidae) infecting intestine of Amur catfish (Silurus asotus).

    abstract::Myxobolus miyairii Kudo, 1919 was first reported from the intestines of the Amur catfish (Silurus asotus) in Japan and then in China and Russia, but with incomplete description. During the investigation of fish myxosporean diversity in Poyang Lake, the biggest lake along the Yangtze River, China, two Amur catfish high...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4889-x

    authors: Liu XH,Zhang JY,Batueva MD,Voronin VN

    更新日期:2016-04-01 00:00:00