A fast and inexpensive method for isolation of high-quality RNA from eimerian oocysts.


:An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h.


Parasitol Res


Parasitology research


Johnston DA,Bratty J,Fernando MA




Has Abstract


1998-07-01 00:00:00












  • Biological activity of Paecilomyces genus against Toxocara canis eggs.

    abstract::Saprophytic soil fungi can exert ovicidal and ovistatic effects on helminths with differing degrees of efficiency. The representatives of such fungi from temperate regions, Paecilomyces lilacinus (Thom) Samson and P. marquandii (Masse) Hughes, exhibit recognized ovicidal activity on some nematodes. We evaluated the ac...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Basualdo JA,Ciarmela ML,Sarmiento PL,Minvielle MC

    更新日期:2000-10-01 00:00:00

  • Continuous in vitro cultivation of Babesia caballi in serum-free medium.

    abstract::Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Zweygarth E,van Niekerk CJ,de Waal DT

    更新日期:1999-05-01 00:00:00

  • Assessment of the role of small mammals in the transmission cycle of tegumentary leishmaniasis and first report of natural infection with Leishmania braziliensis in two sigmodontines in northeastern Argentina.

    abstract::To contribute to the knowledge of the role of small mammals in the transmission cycle of tegumentary leishmaniasis caused by Leishmania braziliensis, we studied the small mammal community and its temporal and spatial association with phlebotominae, as well as small mammal infection by Leishmania spp. by PCR-RFLP analy...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Fernández MS,Fraschina J,Acardi S,Liotta DJ,Lestani E,Giuliani M,Busch M,Salomón OD

    更新日期:2018-02-01 00:00:00

  • Fasciola hepatica: disruption of spermatogenesis by the fasciolicide compound alpha.

    abstract::Sheep infected with the triclabendazole-susceptible Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48, and 72 h post-treatment (p.t.). Changes to the spermatogenic cells in the testis were examined by...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: McConville M,Hanna RE,Brennan GP,McCoy M,Edgar HW,McConnell S,Castillo R,Hernández-Campos A,Fairweather I

    更新日期:2010-01-01 00:00:00

  • Anaplasma phagocytophilum in central and western Wisconsin: a molecular survey.

    abstract::Anaplasma phagocytophilum is an obligate intracellular bacterium that is transmitted to humans through the bite of Ixodes spp. ticks, and causes a febrile disease known as human granulocytic anaplasmosis. The presence of A. phagocytophilum in Wisconsin white-tailed deer blood and in deer ticks was assessed using PCR a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Michalski M,Rosenfield C,Erickson M,Selle R,Bates K,Essar D,Massung R

    更新日期:2006-11-01 00:00:00

  • Size-dependent pattern of metacercariae accumulation in Macomona liliana: the threshold for infection in a dead-end host.

    abstract::While bivalves can acquire trematode metacercariae over their lifetime, the rate at which this accumulation takes place is not necessarily linear. The present study found that the bivalve Macomona liliana acquires very few or no metacercariae until it reaches 30 mm in size, but thereafter the rate at which it becomes ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Leung TL,Poulin R

    更新日期:2008-12-01 00:00:00

  • Effects of orally administered chemotherapeutics (quinine, salinomycin) against Henneguya sp. Thelohán, 1892 (Myxozoa: Myxobolidae), a gill parasite in the tapir fish Gnathonemus petersii Günther, 1862 (Teleostei).

    abstract::When given orally, quinine or salinomycin cause irreversible damage to the plasmodial developmental stages of Henneguya sp., a gill parasite in the tapir fish Gnathonemus petersii. Naturally infected tapir fish measured 75-169 mm in total length and their total weight ranged over 4.3-11.7 g. The fish bore 7-77 plasmod...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Dohle A,Schmahl G,Raether W,Schmidt H,Ritter G

    更新日期:2002-09-01 00:00:00

  • In vitro anti-plasmodial activity of some traditionally used medicinal plants against Plasmodium falciparum.

    abstract::The anti-plasmodial activity of different solvent extracts of Adhatoda vasica (root), Caesalpinia pulcherrima (leaf), Carica papaya (pulp), Erythroxylum monogynum (leaf), Lantana camara (whole plant), Ocimum sanctum (root) and Phyllanthus niruri (whole plant) were studied against Plasmodium falciparum. Of the 35 extra...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Venkatesalu V,Gopalan N,Pillai CR,Singh V,Chandrasekaran M,Senthilkumar A,Chandramouli N

    更新日期:2012-07-01 00:00:00

  • Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequences.

    abstract::Seven isolates of Cryptocaryon irritans from different host species and geographical locations in Mainland China were characterized by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA) using two isolates of Ichthyophthirius multifiliis for comparative purposes. The...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sun HY,Zhu XQ,Xie MQ,Wu XY,Li AX,Lin RQ,Song HQ

    更新日期:2006-07-01 00:00:00

  • Origin, differentiation and functional ultrastructure of egg envelopes in the cestode Echinococcus multilocularis Leuckart, 1863 (Cyclophyllidea: Taeniidae).

    abstract::The origin, differentiation and functional ultrastructure of oncospheral or egg envelopes in Echinococcus multilocularis Leuckart, 1863 were studied by transmission electron microscopy (TEM) and cytochemistry. The purpose of our study is to describe the formation of the four primary embryonic envelopes, namely vitelli...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Świderski Z,Miquel J,Azzouz-Maache S,Pétavy AF

    更新日期:2017-07-01 00:00:00

  • Genetic typing of Echinococcus granulosus in Romania.

    abstract::In Romania, cystic echinococcosis is endemic and affects, besides humans, various animal species including sheep, cattle, and swine. As yet, no molecular-genetic typing has been carried out to clearly identify the putative strains being transmitted there. Parasite samples (protoscoleces or germinal layers) were collec...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bart JM,Morariu S,Knapp J,Ilie MS,Pitulescu M,Anghel A,Cosoroaba I,Piarroux R

    更新日期:2006-01-01 00:00:00

  • A leishmaniosis surveillance system among stray dogs in the region of Madrid: ten years of serodiagnosis (1996-2006).

    abstract::Two seroepidemiological surveys on canine leishmaniosis in stray dogs were performed annually in the Madrid region for 10 years (November 1996-April 2006). The presence of anti-Leishmania antibodies was detected by immunofluorescence antibodies test (cut off 1:100). The overall seroprevalence found in the 1,803 dogs s...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Miró G,Montoya A,Mateo M,Alonso A,García S,García A,Caballero MJ,Molina R

    更新日期:2007-07-01 00:00:00

  • Sarcocystis arctica (Apicomplexa: Sarcocystidae): ultrastructural description and its new host record, the Alaskan wolf (Canis lupus).

    abstract::Sarcocystis sarcocysts are common in muscles of herbivores but are rare in muscles of carnivores. Here, we report sarcocysts in the muscles of a gray wolf (Canis lupus) from Alaska, USA, for the first time. Sarcocysts extracted from the tongue of the wolf were up to 900 μm long and slender and appeared to have a relat...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Calero-Bernal R,Cerqueira-Cézar CK,Verma SK,Mowery J,Carmena D,Beckmen K,Dubey JP

    更新日期:2016-07-01 00:00:00

  • Application of the recombinant Theileria annulata surface protein in an indirect ELISA for the diagnosis of tropical theileriosis.

    abstract::The recombinant surface protein of Theileria annulata (TaSP) was used in the standardization and validation of an enzyme linked immunosorbent assay (ELISA) for the detection of circulating antibodies against tropical theileriosis. ELISA data were expressed as the percentage positivity (PP) of the reactivity of an inte...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bakheit MA,Schnittger L,Salih DA,Boguslawski K,Beyer D,Fadl M,Ahmed JS

    更新日期:2004-03-01 00:00:00

  • Prime-boost: the way forward for recombinant vaccines against apicomplexan parasites. A Theileria perspective.

    abstract::A good CD8+ response is required for immunity to many intracellular pathogens. Traditional antigen delivery using isolated sub-units or killed pathogens and adjuvant stimulates strong antibody responses but weak T-cell reactivity. Attenuated vaccines are usually more effective. The prime-boost delivery system based on...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Hall R,Adamson R,Boulter N

    更新日期:2002-05-01 00:00:00

  • Occurrence of Anisakis and Hysterothylacium larvae in commercial fish from Balearic Sea (Western Mediterranean Sea).

    abstract::This study investigates the occurrence of anisakids and raphidascarids in commercial fish from Balearic Sea (Western Mediterranean). A total of 335 fish including 19 black anglerfish (Lophius budegassa), 33 white anglerfish (L. piscatorius), 129 European hake (Merluccius merluccius), 30 red mullet (Mullus barbatus), a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Barcala E,Ramilo A,Ortega N,Picó G,Abollo E,Pascual S,Muñoz P

    更新日期:2018-12-01 00:00:00

  • Oviposition deterrent and ovicidal activities of seven herbal essential oils against female adults of housefly, Musca domestica L.

    abstract::The oviposition deterrent and ovicidal of seven herbal essential oils derived from Citrus sinensis, Cymbopogon citratus, Eucalyptus glubulus, Illicium verum, Lavandula angustifolia, Mentha piperita, and Zingiber cussumunar were assessed against the gravid female of housefly, Musca domestica L., under laboratory condit...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sinthusiri J,Soonwera M

    更新日期:2014-08-01 00:00:00

  • The association of Blastocystis hominis and Endolimax nana with diarrheal stools in Zambian school-age children.

    abstract::To determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the Southern Province of Zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). Almost half of the stools (49.5%) were diarrhetic. The overal...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Graczyk TK,Shiff CK,Tamang L,Munsaka F,Beitin AM,Moss WJ

    更新日期:2005-12-01 00:00:00

  • Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene.

    abstract::Twenty-five isolates of Echinococcus granulosus protoscoleces from hydatid cysts were collected from 25 buffaloes in five different provinces of Iran. DNA was extracted from protoscoleces and used for phylogenetical analysis. For this aim, the DNA was amplified using specific primers derived from cox1 gene and, subseq...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Pour AA,Hosseini SH,Shayan P

    更新日期:2011-05-01 00:00:00

  • Seasonal occurrence and metrical variability of Gyrodactylus rhodei Zitnan 1964 (Monogenea, Gyrodactylidae).

    abstract::The seasonal dynamics of Gyrodactylus rhodei, a monogenean ectoparasite of bitterling (Rhodeus sericeus), was studied from June 2000 to May 2001 in the Kyjovka River, Czech Republic. A negative relationship between prevalence and intensity of infection of G. rhodei and water temperature was found. Metrical variability...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Dávidová M,Jarkovský J,Matejusová I,Gelnar M

    更新日期:2005-04-01 00:00:00

  • Hirudins and hirudin-like factors in Hirudinidae: implications for function and phylogenetic relationships.

    abstract::Haematophagous leeches express a broad variety of bioactive factors that are released from the salivary gland cells into the wound of a host during feeding. Among these, hirudin is probably the best studied factor and, moreover, the only one that has successfully made the transition from nature to clinical use. Many c...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Müller C,Haase M,Lemke S,Hildebrandt JP

    更新日期:2017-01-01 00:00:00

  • Correction to: Acanthamoeba of three morphological groups and distinct genotypes exhibit variable and weakly inter-related physiological properties.

    abstract::The authors recognized a mistake in Abstract and in the Discussion section. ...

    journal_title:Parasitology research

    pub_type: 杂志文章,已发布勘误


    authors: Possamai CO,Loss AC,Costa AO,Falqueto A,Furst C

    更新日期:2018-06-01 00:00:00

  • Thelohania montirivulorum sp. nov. (Microspora: Thelohaniidae), a parasite of the Australian freshwater crayfish, Cherax destructor (Decapoda: Parastacidae): fine ultrastructure, molecular characteristics and phylogenetic relationships.

    abstract::Thelohania montirivulorum sp. nov., a new species of microsporidian parasite, was found in a highland population of the Australian yabby, Cherax destructor. Data are presented on fine ultrastructure, developmental morphology and DNA sequence of the small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Moodie EG,Le Jambre LF,Katz ME

    更新日期:2003-10-01 00:00:00

  • The involvement of the cysteine proteases of Clonorchis sinensis metacercariae in excystment.

    abstract::The effects of trypsin, bile, trypsin-bile, pepsin, dithiothreitol (DTT) and metacercarial excretory-secretory product (ESP) on the in vitro excystment of Clonorchis sinensis metacercariae were investigated. The majority of metacercariae excysted immediately in trypsin-bile in PBS solution, a process which was complet...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Li S,Chung YB,Chung BS,Choi MH,Yu JR,Hong ST

    更新日期:2004-05-01 00:00:00

  • Studies on the impact of biosynthesized silver nanoparticles (AgNPs) in relation to malaria and filariasis vector control against Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae).

    abstract::Biosynthesized nanoparticles have been achieved using environmentally acceptable plant extract and eco-friendly reducing and capping agents. The present study was based on assessments of the larvicidal activities to determine the efficacies of synthesized silver nanoparticles (AgNPs) using aqueous leaf extract of Vinc...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Subarani S,Sabhanayakam S,Kamaraj C

    更新日期:2013-02-01 00:00:00

  • Occurrence of trichodinid ciliates (Peritricha : Urceolariidae) in the Kiel Fjord, Baltic Sea, and its possible use as a biological indicator.

    abstract::Investigations on the occurrence of trichodinid ciliates from fish caught in the Kiel Bight and Kiel Fjord (western Baltic Sea) were carried out between September 1996 and March 1997. Smears of the gills, fins, and skin of 120 Gadus morhua and 92 Platichthys flesus caught by fish traps and trammel nets revealed the pr...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Palm HW,Dobberstein RC

    更新日期:1999-08-01 00:00:00

  • Characterization and differential expression analysis of Toxocara canis aquaporin-1 gene.

    abstract::Toxocara canis is an intestinal nematode of canids with a worldwide distribution, causing an important but neglected parasitic zoonosis in humans. Aquaporins (AQP) are a family of water channel proteins, which function as membrane channels to regulate water homeostasis. In this study, the coding sequence of aquaporin-...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Luo YF,Hu L,Ma GX,Luo YL,Yin SS,Xiong Y,Zhu XQ,Zhou RQ

    更新日期:2016-09-01 00:00:00

  • Two simple techniques for the safe Sarcoptes collection and individual mite DNA extraction.

    abstract::Availability of mites is a recognized limiting factor of biological and genetic investigations of the genus Sarcoptes. Current methods of deoxyribonucleic acid (DNA) extraction from individual mites also need substantial improvement in efficiency and operator friendliness. We have first developed a technique for effic...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Soglia D,Rambozzi L,Maione S,Spalenza V,Sartore S,Alasaad S,Sacchi P,Rossi L

    更新日期:2009-10-01 00:00:00

  • Biological nature of Cryptosporidium sp. isolated from a cat.

    abstract::A small type of Cryptosporidium oocysts was isolated from a naturally infected cat and its biological nature was investigated. In cats experimentally inoculated with Cryptosporidium oocysts, long-lasting shedding of the oocysts occurred after a prepatent period of 8-10 days, and a number of peaks of oocyst count appea...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Asahi H,Koyama T,Arai H,Funakoshi Y,Yamaura H,Shirasaka R,Okutomi K

    更新日期:1991-01-01 00:00:00

  • Metabolites from the leaf extract of Serjania yucatanensis with trypanocidal activity against Trypanosoma cruzi.

    abstract::The bioassay-guided phytochemical investigation of the leaf extract of Serjania yucatanensis, a woody climbing plant endemic to the Yucatan peninsula, led to the identification of a mixture of a triterpene [lup-20(29)-en-3-one] and an oxygenated sesquiterpene (β-caryophyllene oxide), as that responsible for the origin...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Polanco-Hernández G,Escalante-Erosa F,García-Sosa K,Chan-Bacab MJ,Sagua-Franco H,González J,Osorio-Rodríguez L,Peña-Rodríguez LM

    更新日期:2012-07-01 00:00:00