Abstract：
:Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Abdel-Shafi IR,Shoieb EY,Attia SS,Rubio JM,Ta-Tang TH,El-Badry AAdoi
10.1007/s00436-017-5372-7subject
Has Abstractpub_date
2017-03-01 00:00:00pages
963-970issue
3eissn
0932-0113issn
1432-1955pii
10.1007/s00436-017-5372-7journal_volume
116pub_type
杂志文章abstract：:Cryptosporidiosis, caused by Cryptosporidium, causes self-limited diarrhea in normal hosts but may cause life-threatening diarrhea in immunocompromised persons. Cryptosporidium-induced manifestations, including weight-loss and intestinal physiological alterations are not noted in adult immunocompetent mice. So far, st...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0785-3
更新日期：2008-02-01 00:00:00
abstract：:Exposure to viable Acanthamoeba may cause fatal encephalitis and blinding keratitis in humans. Quantification of environmental Acanthamoeba by a reliable analytical assay is essential to assess the risk of human exposure and efficacy of control measures (e.g., superheating). Two DNA binding dyes (ethidium monoazide (E...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3556-3
更新日期：2013-11-01 00:00:00
abstract：:Schistosomiasis japonica remains one important public health concern that cause great loss of humans' health and social-economic development in the Peoples' Republic of China. At the end of 1990s and the beginning of 2000s, there were still about 0.8 million patients and nearly 85 million people living in the epidemic...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3295-5
更新日期：2013-04-01 00:00:00
abstract：:The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050435
更新日期：1998-06-01 00:00:00
abstract：:Ras are key components of diverse signal transduction pathways and play important roles in growth and development. To know about growth regulation in Clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cDNA library. The open reading frame contains 561 bp ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2147-9
更新日期：2011-04-01 00:00:00
abstract：:Chagas disease (CD) is a tropical zoonosis caused by the protozoan Trypanosoma cruzi. Severe autonomic dysfunction like reduced cardiac catecholamine-containing or acetylcholinesterase-positive innervation have been reported in CD. Renin-angiotensin system (RAS) seems to participate in the regulation of adrenal catech...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06485-6
更新日期：2020-01-01 00:00:00
abstract：:Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4327-0
更新日期：2015-04-01 00:00:00
abstract：:Serum and colostrum were collected from adult buffalo cows naturally infected with Toxocara vitulorum. When injected into mice, the colostrum reduced the number of larvae of T. vitulorum that migrated in the mice. Injection of particularly the IgG-containing fraction but also the IgM-containing fraction of Sephadex G2...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932381
更新日期：1994-01-01 00:00:00
abstract：:The round goby Neogobius melanostomus is a bottom-dwelling fish native for the Ponto-Caspian basin, which started to colonize the Baltic Sea since 1991. The parasites of this fish species in the southwestern Baltic region are studied for the first time. The round goby in the SW Baltic Sea was infected with 12 parasite...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2321-8
更新日期：2011-09-01 00:00:00
abstract：:To determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the Southern Province of Zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). Almost half of the stools (49.5%) were diarrhetic. The overal...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0003-0
更新日期：2005-12-01 00:00:00
abstract：:The bacteria Anaplasma platys, Anaplasma phagocytophilum and Ehrlichia canis are tick-borne agents that cause canine vector-borne disease. The prevalence of these pathogens in South Eastern Europe is unknown with the exception of an isolated case of A. platys detected in a dog imported into Germany from Croatia. To ga...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5611-y
更新日期：2017-11-01 00:00:00
abstract：:Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Satureja cuneifolia and Melissa officinalis. In th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2744-2
更新日期：2012-06-01 00:00:00
abstract：:The uterine organization in Tetrabothrius erostris (Tetrabothriidea) was investigated by the methods of transmission and scanning electron microscopy. In sexually mature proglottids, the uterine wall consists of a syncytial epithelium (1.4-2.5 μm thick, except in regions containing nuclei). The ribosomes, mitochondria...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-4153-9
更新日期：2014-12-01 00:00:00
abstract：:Nematodes of the family Aspidoderidae (Nematoda: Heterakoidea) Freitas 1956 are widely distributed from Americas. The species of the genus Aspidodera Railliet and Henry 1912 are parasites of mammals of the orders Edentata, Marsupialia, and Rodentia. In the present work, Aspidodera raillieti (L. Travassos, Mem Inst Osw...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0641-5
更新日期：2007-10-01 00:00:00
abstract：:The effect of incubation of parasites (epimastigote and trypomastigote forms of Trypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process of N-glycosylation of p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930829
更新日期：1989-01-01 00:00:00
abstract：:NADH-ubiquinone oxidoreductase activity is present in mitochondrial lysates of Phytomonas serpens. Rotenone at 2-10 microM inhibited the activity 50-75%, indicating that it belongs to respiratory complex I. The activity was also inhibited 50-60% in the presence of 10-30 nM atovaquone suggesting that inhibition of comp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1058-4
更新日期：2004-03-01 00:00:00
abstract：:The oocysts of Caryospora cherrughi sp. n. were described from the feces of the saker falcon, Falco cherrug, collected from the falcon market in Riyadh City, Saudi Arabia. The prevalence of infection was 21.4 % (15/70). The majority of the oocysts examined had completed sporulation within 64-84 h at 24 ± 2 °C. Sporula...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3217-y
更新日期：2013-03-01 00:00:00
abstract：:According to the genetic characterization by the analysis of the miniexon gene, strains of Trypanosoma cruzi can be classified into six discrete typing units (DTUs), and the DTU 1 into four distinct genotypes associated with different life cycles. While Chagas disease is endemic in Costa Rica, T. cruzi isolates from t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3000-0
更新日期：2012-10-01 00:00:00
abstract：:We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1304-x
更新日期：2009-03-01 00:00:00
abstract：:In the State of Amazonas, American tegumentary leishmaniasis is endemic and presents a wide spectrum of clinical variability due to the large diversity of circulating species in the region. Isolates from patients in Manaus and its metropolitan region were characterized using monoclonal antibodies and isoenzymes belong...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2139-9
更新日期：2011-03-01 00:00:00
abstract：:Many malaria-endemic areas are also associated with high rates of human immunodeficiency virus (HIV) infection. An understanding of the chemotherapeutic interactions that occur during malaria and HIV co-infections is important. Our previous studies have demonstrated that some antiretroviral protease inhibitors are eff...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2427-z
更新日期：2011-12-01 00:00:00
abstract：:Suckling rabbits from six litters were orally inoculated at various ages with oocysts of Eimeria flavescens or Eimeria intestinalis. The total number of oocysts in the ceca served to estimate parasite reproduction. The rabbits at 14 and 16 days old remained uninfected, and in 19-day-old animals, low or no oocyst shedd...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0646-0
更新日期：2007-10-01 00:00:00
abstract：:The efficacy of emodepside plus toltrazuril (Procox® oral suspension for dogs) against different species of gastrointestinal nematodes (Toxocara canis, Ancylostoma caninum, Uncinaria stenocephala) was evaluated in nine randomised,blinded and placebo-controlled laboratory studies in naturally or experimentally infected...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-011-2397-1
更新日期：2011-08-01 00:00:00
abstract：:The nervous system of Cysticercus cellulosae, the metacestode stage of the tapeworm Taenia solium, was delineated using histochemical methods for the localization of the enzyme markers; nonspecific esterase and acetylcholinesterase. The main features of the nervous system include a pair of cerebral ganglia, a circumce...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00936456
更新日期：1992-01-01 00:00:00
abstract：:The emergence of resistance against most of the drugs in current use against malaria has aggravated the disease burden in endemic regions. Several plants species have been used for treatment of malaria in traditional/cultural health systems. Bergenia ciliata, used traditionally for treatment of fever by local communit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3487-z
更新日期：2013-09-01 00:00:00
abstract：:Malarial antigens on the surface of infected erythrocytes have been described by many investigators. However, few of these antigens have been unambiguously demonstrated to be exposed on the surface of erythrocytes. This study demonstrates that mild glutaraldehyde fixation results in the cytoplasmic face of the host me...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932242
更新日期：1993-01-01 00:00:00
abstract：:In the current laboratory study, 14 essential oils (EOs) derived from 12 Lamiaceae plant species and their major components were screened for their larvicidal and repellent properties against Aedes albopictus, an invasive mosquito species of great medical importance. The results of toxicity bioassays revealed that the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5892-9
更新日期：2018-06-01 00:00:00
abstract：:Systematic, continuous mosquito surveillance is considered the most reliable tool to predict the spread and establishment of alien mosquito species such as the Asian tiger mosquito (Aedes albopictus), Japanese bush mosquito (Aedes japonicus), and the transmission risk of mosquito-borne arboviruses to humans. Only sing...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06287-w
更新日期：2019-05-01 00:00:00
abstract：:In 1997, an outbreak of alveolar echinococcosis in Japanese monkeys (Macaca fuscata) occurred in a zoo in Hokkaido, Japan. Twelve infected monkeys from a colony (n = 57) were diagnosed serologically by Western blotting, and ultrasonography showed the presence of tumor-like tissue in the livers of nine monkeys. The 12 ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1627-2
更新日期：2009-12-01 00:00:00
abstract：:Transferrin is a defence protein known to be up-regulated upon infection of parasites/pathogens in Aedes aegypti mosquito. However, no information is available on its up-regulation in Culex quinquefasciatus, the vector of bancroftian filarial parasite. In the present study, enhancement of transferrin in C. quinquefasc...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0474-2
更新日期：2007-07-01 00:00:00
