Detection of Cryptosporidium spp. from human faeces by PCR-RFLP, cloning and sequencing.

Abstract:

:In this study, we compared and validated a nested ABC polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay to amplify Cryptosporidium parvum oocyst wall protein (COWP) gene fragment with a previous nested PCR-RFLP method. When RFLP of COWP gene did not provide clear results, we further analysed samples by cloning and sequencing for species and genotype identification. ABC-PCR was performed on DNA extracted from human faecal samples collected in England where Cryptosporidium was previously detected by conventional methods. COWP gene amplification was successful for all specimens; RFLP analyses using both RsaI and AluI enzymes recognised 54/55 restriction profiles. Among these, 40 (72.7%) characteristic patterns were generated for C. parvum and six (10.9%) for Cryptosporidium hominis; four (7.3%) showed species mixtures, while the remaining four samples (7.3%) showed atypical genotypes. Cloning and sequencing of mixtures and atypical genotypes identified one C. parvum, one C. hominis and three Cryptosporidium meleagridis. Indeed, molecular tools improved PCR-RFLP results. In conclusion, this study showed that ABC-PCR-RFLP technique was quick, simple and specific. Cloning and sequencing were helpful in characterising mixtures or previously uncharacterised RFLP patterns. All these epidemiological findings are useful information for any preventive intervention to control disease diffusion.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Leone A,Ripabelli G,Sammarco ML,Grasso GM

doi

10.1007/s00436-008-1233-8

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

583-7

issue

3

eissn

0932-0113

issn

1432-1955

journal_volume

104

pub_type

杂志文章
  • Plasmodium vivax and P. chabaudi: inter-species cross-reacting antigens.

    abstract::A monoclonal antibody raised by immunization of BALB/c mice with erythrocytic stages of Plasmodium vivax was shown to react with asexual erythrocytic stages of P. chabaudi. The cross-reactivity molecules are antigens of 200 and 148 kDa in P. vivax and of 190 and 70 kDa in P. chabaudi. Immunofluorescence studies of the...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050241

    authors: Perez HA,Bracho C,Romano M,De la Rosa M

    更新日期:1997-01-01 00:00:00

  • Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Cestoda, Nippotaeniidea): first data on spermiogenesis and sperm ultrastructure.

    abstract::Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-t...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4327-0

    authors: Bruňanská M,Bílý T,Nebesářová J

    更新日期:2015-04-01 00:00:00

  • Trypanosoma cruzi: evaluation of (-)-cubebin derivatives activity in the messenger RNAs processing.

    abstract::No fully effective treatment has been developed since the discovery of Chagas' disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to n...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2275-x

    authors: Andrade e Silva ML,Cicarelli RM,Pauletti PM,Luz PP,Rezende KC,Januário AH,da Silva R,Pereira AC,Bastos JK,de Albuquerque S,Magalhães LG,Cunha WR

    更新日期:2011-08-01 00:00:00

  • Competitive growth, energy allocation, and host modification in the acanthocephalan Acanthocephalus dirus: field data.

    abstract::The acanthocephalan Acanthocephalus dirus is a trophically transmitted parasite that modifies both the physiology and behavior of its intermediate host (isopod) prior to transmission to its definitive host (fish). Infected isopods often contain multiple A. dirus individuals and we examined the relationships between ho...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-5279-8

    authors: Caddigan SC,Pfenning AC,Sparkes TC

    更新日期:2017-01-01 00:00:00

  • Assessment of the role of small mammals in the transmission cycle of tegumentary leishmaniasis and first report of natural infection with Leishmania braziliensis in two sigmodontines in northeastern Argentina.

    abstract::To contribute to the knowledge of the role of small mammals in the transmission cycle of tegumentary leishmaniasis caused by Leishmania braziliensis, we studied the small mammal community and its temporal and spatial association with phlebotominae, as well as small mammal infection by Leishmania spp. by PCR-RFLP analy...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5714-5

    authors: Fernández MS,Fraschina J,Acardi S,Liotta DJ,Lestani E,Giuliani M,Busch M,Salomón OD

    更新日期:2018-02-01 00:00:00

  • Development of an indirect immunofluorescence technique for the diagnosis of toxoplasmosis in bottlenose dolphins.

    abstract::The diagnosis of toxoplasmosis is often complicated by the lack of specific clinical symptoms or postmortem features, in humans and other animals. The only diagnostic test described so far for the serological diagnosis of Toxoplasma gondii in marine mammals is the modified agglutination test (Dubey et al., Am J Vet Re...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3674-y

    authors: Bernal-Guadarrama MJ,Salichs J,Almunia J,García-Parraga D,Fernández-Gallardo N,Santana-Morales MÁ,Pacheco V,Afonso-Lehmann RN,Déniz D,Lorenzo-Morales J,Valladares B,Martínez-Carretero E

    更新日期:2014-02-01 00:00:00

  • Host-feeding pattern of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in heterogeneous landscapes of South Andaman, Andaman and Nicobar Islands, India.

    abstract::Mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. Therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. Competent vectors of dengue and chikungunya viz. Aedes aegypti and Aedes albo...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4634-5

    authors: Sivan A,Shriram AN,Sunish IP,Vidhya PT

    更新日期:2015-09-01 00:00:00

  • Studies on tsetse midgut factors that induce differentiation of blood-stream Trypanosoma brucei brucei in vitro.

    abstract::An in vitro system for studying the transformation of bloodstream forms of Trypanosoma brucei brucei into procylic (midgut) forms is described. In this system, transformation of the parasites was stimulated by Glossina morsitans morsitans midgut homogenates at 27 degrees C but not at 4 degrees C. The transformation-st...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00936174

    authors: Imbuga MO,Osir EO,Labongo VL,Darji N,Otieno LH

    更新日期:1992-01-01 00:00:00

  • A survey of the prevalence and genotypes of Giardia duodenalis infecting household and sheltered dogs.

    abstract::Giardia duodenalis (syn. G. intestinalis, G. lamblia) is an intestinal protozoan parasite that infects humans and a wide range of mammals that includes dogs. Highly significant genetic heterogeneity has been found within this species, while only genotypes from assemblages A and B have zoonotic potential. Although Giar...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-1766-5

    authors: Solarczyk P,Majewska AC

    更新日期:2010-04-01 00:00:00

  • Toxoplasma gondii metacaspase 2 is an important factor that influences bradyzoite formation in the Pru strain.

    abstract::Toxoplasma gondii is an important zoonotic protozoan of the phylum Apicomplexa that can infect nearly all warm-blooded animals. The parasite can exist as the interconvertible tachyzoite or bradyzoite forms, leading to acute or latent infection, respectively. No drug has been reported to penetrate the cyst wall and red...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-020-06722-3

    authors: Song X,Lin M,Li M,Yang X,Liu J,Liu Q

    更新日期:2020-07-01 00:00:00

  • Factors affecting the component community structure of haemoparasites in common voles ( Microtus arvalis) from the Mazury Lake District region of Poland.

    abstract::The prevalence and abundance of infections with haemoparasites were studied over a 4-year period in Microtus arvalis (common vole, n =321) sampled from fallow grassland sites in north-eastern Poland. Total species richness was five (prevalence= Haemobartonella sp. 63.9%, Bartonella spp. 27.7%, Babesia microti 9.0%, Tr...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-003-1040-1

    authors: Pawelczyk A,Bajer A,Behnke JM,Gilbert FS,Sinski E

    更新日期:2004-03-01 00:00:00

  • Short communication on regional climate change scenarios and their possible use for impact studies on vector-borne diseases.

    abstract::Atmospheric observations demonstrate that, during the last decades, the climate has changed. As reported by the Intergovernmental Panel on Climate Change (IPCC, 2001, 2007), a mean increase of temperature by 0.09 K per decade was observed globally from 1951 to 1989. Up to now, 2008, this trend has continued. Europe ex...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-1099-9

    authors: Jacob D

    更新日期:2008-12-01 00:00:00

  • Prostate adenocarcinoma associated with prostatic infection due to Schistosoma haematobium. Case report and systematic review.

    abstract::Schistosomiasis affects more than 240 million people worldwide, an infection which may cause urogenital manifestations including, among others, squamous bladder cancer and prostate involvement. We describe the first case of a prostate adenocarcinoma associated with prostatic Schistosoma haematobium infection occurring...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审

    doi:10.1007/s00436-014-4250-9

    authors: Figueiredo JC,Richter J,Borja N,Balaca A,Costa S,Belo S,Grácio MA

    更新日期:2015-02-01 00:00:00

  • Evora district as a new focus for canine leishmaniasis in Portugal.

    abstract::On the basis of information acquired from local health authorities in Evora district of Portugal on cases of visceral leishmaniasis (VL), an epidemiology survey study was conducted. To determine the prevalence of anti-Leishmania antibodies in the local human and canine populations residing in Evora town and 14 adjacen...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00937115

    authors: Semião-Santos SJ,el Harith A,Ferreira E,Pires CA,Sousa C,Gusmão R

    更新日期:1995-01-01 00:00:00

  • Predominance of Blastocystis sp. subtype 4 in rural communities, Nepal.

    abstract::Blastocystis sp. is a common intestinal parasite. To date, there have been sporadic and scanty studies on Blastocystis sp. carried out in rural communities in Nepal. We surveyed the prevalence of Blastocystis sp. and its possible associated risk factors, and reported the predominant Blastocystis sp. subtype in two rur...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2665-0

    authors: Lee IL,Tan TC,Tan PC,Nanthiney DR,Biraj MK,Surendra KM,Suresh KG

    更新日期:2012-04-01 00:00:00

  • Comparative study of nitric oxide (NO) production during human hydatidosis: relationship with cystic fluid fertility.

    abstract::Human hydatidosis is characterized by a prolonged coexistence of Echinococcus granulosus and its host without effective rejection of the parasite. This parasitic infection constitutes a major health problem in Algeria. In this study, we investigated in vivo production of nitrite (NO(2)(-) + NO(3)(-)) in sera of Algeri...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-012-3181-6

    authors: Zeghir-Bouteldja R,Amri M,Bouaziz S,Mezioug D,Touil-Boukoffa C

    更新日期:2013-02-01 00:00:00

  • Metazoan parasites and food composition of juvenile Etmopterus spinax (L., 1758) (Dalatiidae, Squaliformes) from the Norwegian Deep.

    abstract::A total of 37 juvenile Etmopterus spinax from the Norwegian Deep were examined for stomach contents and metazoan ecto- and endoparasites. These squaliform elasmobranchs were caught by benthopelagic net in May 2001 at a depth of 194-214 m. The euphausiid Meganyctiphanes norvegica and the teleost Maurolicus muelleri wer...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-002-0741-1

    authors: Klimpel S,Palm HW,Seehagen A

    更新日期:2003-03-01 00:00:00

  • Eimeria bovis infection enhances adhesion of peripheral blood mononuclear cells to and their transmigration through an infected bovine endothelial cell monolayer in vitro.

    abstract::The first schizogony of Eimeria bovis takes place in lymphatic endothelial cells of the ileum, resulting in the formation of macroschizonts within 2-3 weeks. In this study, we analyse early cellular immune responses to infected host cells on the basis of peripheral blood mononuclear cell (PBMC) adhesion on and transmi...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0517-8

    authors: Taubert A,Zahner H,Hermosilla C

    更新日期:2007-08-01 00:00:00

  • The role of free-ranging, captive, and domestic birds of Western Poland in environmental contamination with Cryptosporidium parvum oocysts and Giardia lamblia cysts.

    abstract::As Cryptosporidium parvum and Giardia lamblia can be disseminated in the environment by avian hosts, a total of 499 fecal dropping from 308 free-ranging, 90 captive, and 101 domestic birds were tested by conventional, immunological, and molecular techniques for these human enteropathogens. Twenty-six (5.2%) tested pos...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-1293-9

    authors: Majewska AC,Graczyk TK,Słodkowicz-Kowalska A,Tamang L,Jedrzejewski S,Zduniak P,Solarczyk P,Nowosad A,Nowosad P

    更新日期:2009-04-01 00:00:00

  • Echinococcus multilocularis metacestode metabolites contain a cysteine protease that digests eotaxin, a CC pro-inflammatory chemokine.

    abstract::In many helminthic infections, eotaxin, a CC-chemokine, triggers the mobilization of eosinophils, thus, contributing to an elevated blood and periparasitic eosinophil level. Following an experimental intraperitoneal infection of C57BL6 mice with Echinococcus multilocularis metacestodes, however, we observed the absenc...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-009-1549-z

    authors: Mejri N,Gottstein B

    更新日期:2009-10-01 00:00:00

  • Prevalence of camel trypanosomosis (surra) and associated risk factors in Borena zone, southern Ethiopia.

    abstract::A study was made to determine the prevalence of camel trypanosomosis (surra) and its associated risk factors in Borena zone, southern Ethiopia during 2013-2014. A total of 2400 blood samples were collected and examined by the buffy coat and thin blood film laboratory methods, and data were analyzed using the SPSS stat...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4845-9

    authors: Olani A,Habtamu Y,Wegayehu T,Anberber M

    更新日期:2016-03-01 00:00:00

  • Amplified fragment length polymorphism: an adept technique for genome mapping, genetic differentiation, and intraspecific variation in protozoan parasites.

    abstract::With the advent of polymerase chain reaction (PCR), genetic markers are now accessible for all organisms, including parasites. Amplified fragment length polymorphism (AFLP) is a PCR-based marker for the rapid screening of genetic diversity and intraspecific variation. It is a potent fingerprinting technique for genomi...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审

    doi:10.1007/s00436-012-3238-6

    authors: Kumar A,Misra P,Dube A

    更新日期:2013-02-01 00:00:00

  • Evaluation of quantitative real-time PCR for rapid assessments of the exposure of sentinel fish to Myxobolus cerebralis.

    abstract::Pathogen-free rainbow trout (Oncorhynchus mykiss) aged 735 degree days were experimentally exposed to a low dose of infectious Myxobolus cerebralis (20 triactinomyxons fish(-1)). Three time periods were chosen for sampling that included 10 days (d), 67 d, and 5 months (mo) post exposure. Five diagnostic assays were us...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-006-0166-3

    authors: Kelley GO,Adkison MA,Zagmutt-Vergara FJ,Leutenegger CM,Bethel JW,Myklebust KA,McDowell TS,Hedrick RP

    更新日期:2006-09-01 00:00:00

  • Inhibitory monoclonal antibodies to soluble Plasmodium falciparum antigens.

    abstract::Several murine monoclonal antibodies were raised against purified soluble Plasmodium falciparum antigens from the asexual blood stage. The monoclonal antibodies were purified from ascites by preparative agarose gel electrophoresis and tested for inhibitory activities against P. falciparum in vitro cultures. One monocl...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00535326

    authors: Jakobsen PH,Jepsen S,Agger R

    更新日期:1987-01-01 00:00:00

  • Seaweeds as a source of lead compounds for the development of new antiplasmodial drugs from South East coast of India.

    abstract::The problems of resistant lines of Plasmodium falciparum are escalating. Twelve seaweeds species belong to five different families (Sargassaceae, Gracilariaceae, Hypneaceae, Corallinaceae and Halimedaceae) were collected from Mandapam coastal area, and the seaweeds extracts were tested for in vitro antiplasmodial acti...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-2219-x

    authors: Ravikumar S,Jacob Inbaneson S,Suganthi P

    更新日期:2011-07-01 00:00:00

  • Isolation and identification of Acanthamoeba species from natural water sources in the northeastern part of Thailand.

    abstract::Acanthamoeba are found in the environment, particularly in water, all over the world. The genus is currently classified into 20 different genotypes, T1-T20. In this study, 63 natural water samples from 11 provinces in northeast Thailand were collected and cultured on non-nutrient agar plates. Positive samples by cultu...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-4911-y

    authors: Thammaratana T,Laummaunwai P,Boonmars T,Neglected, zoonosis and vector-borne disease research group.

    更新日期:2016-04-01 00:00:00

  • Anti-trypanosomal activities and structural chemical properties of selected compound classes.

    abstract::Potent compounds do not necessarily make the best drugs in the market. Consequently, with the aim to describe tools that may be fundamental for refining the screening of candidates for animal and preclinical studies and further development, molecules of different structural classes synthesized within the frame of a br...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-4210-4

    authors: Ponte-Sucre A,Bruhn H,Schirmeister T,Cecil A,Albert CR,Buechold C,Tischer M,Schlesinger S,Goebel T,Fuß A,Mathein D,Merget B,Sotriffer CA,Stich A,Krohne G,Engstler M,Bringmann G,Holzgrabe U

    更新日期:2015-02-01 00:00:00

  • Phylogenetic characterization of Setaria equina and its association with other filarids.

    abstract::Molecular characterization studies on Setaria equina are limited. The present study aimed to characterize S. equina at the cytochrome c oxidase gene and to examine its phylogenetic relationships with other filarid species. Sequence analysis showed 100% nucleotide homology with an S. equina sequence from Italy (AJ54487...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-020-06917-8

    authors: Devi A,Sudan V,Shanker D

    更新日期:2020-12-01 00:00:00

  • A retrospective survey into the presence of Plasmodium spp. and Toxoplasma gondii in archived tissue samples from New Zealand raptors: New Zealand falcons (Falco novaeseelandiae), Australasian harriers (Circus approximans) and moreporks (Ninox novaeseelan

    abstract::Human colonisation of New Zealand has resulted in the introduction of emerging diseases, such as avian malaria and toxoplasmosis, which arrived with their exotic avian and mammalian hosts. Plasmodium spp. and Toxoplasma gondii have a wide host range, and several species of endemic New Zealand birds have developed a fa...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5536-5

    authors: Mirza V,Burrows EB,Gils S,Hunter S,Gartrell BD,Howe L

    更新日期:2017-08-01 00:00:00

  • Molecular characterization of Cyclospora-like organism from dairy cattle.

    abstract::Cyclospora cayetanensis was identified as the cause of large outbreaks of diarrhea in many parts of the world, but its host range and reservoirs remains poorly defined. Recently, oocysts resembling the C. cayetanensis were detected in dairy cattle fecal specimens from China. The 18S rDNA from two of these Cyclospora-l...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-006-0380-z

    authors: Li G,Xiao S,Zhou R,Li W,Wadeh H

    更新日期:2007-04-01 00:00:00