Abstract：
:In this study, we compared and validated a nested ABC polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay to amplify Cryptosporidium parvum oocyst wall protein (COWP) gene fragment with a previous nested PCR-RFLP method. When RFLP of COWP gene did not provide clear results, we further analysed samples by cloning and sequencing for species and genotype identification. ABC-PCR was performed on DNA extracted from human faecal samples collected in England where Cryptosporidium was previously detected by conventional methods. COWP gene amplification was successful for all specimens; RFLP analyses using both RsaI and AluI enzymes recognised 54/55 restriction profiles. Among these, 40 (72.7%) characteristic patterns were generated for C. parvum and six (10.9%) for Cryptosporidium hominis; four (7.3%) showed species mixtures, while the remaining four samples (7.3%) showed atypical genotypes. Cloning and sequencing of mixtures and atypical genotypes identified one C. parvum, one C. hominis and three Cryptosporidium meleagridis. Indeed, molecular tools improved PCR-RFLP results. In conclusion, this study showed that ABC-PCR-RFLP technique was quick, simple and specific. Cloning and sequencing were helpful in characterising mixtures or previously uncharacterised RFLP patterns. All these epidemiological findings are useful information for any preventive intervention to control disease diffusion.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Leone A,Ripabelli G,Sammarco ML,Grasso GMdoi
10.1007/s00436-008-1233-8subject
Has Abstractpub_date
2009-02-01 00:00:00pages
583-7issue
3eissn
0932-0113issn
1432-1955journal_volume
104pub_type
杂志文章abstract：:To determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the Southern Province of Zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). Almost half of the stools (49.5%) were diarrhetic. The overal...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0003-0
更新日期：2005-12-01 00:00:00
abstract：:Before and after a stress treatment, larval stages and adult worms of Haemonchus contortus were tested for the induction and expression of heat-shock proteins (HSPs) using immunoblot analysis with HSP70-and HSP65-specific monoclonal antibodies. Stress treatment or heat shock did not alter the signals obtained with the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931852
更新日期：1995-01-01 00:00:00
abstract：:Gyrodactylus bubyri Osmanov, 1965, a monogenean parasite of Caucasian dwarf goby Knipowitschia caucasica (Berg) described from Aral Sea and subsequently reported from the same host from Strymon River, Greece, is recorded from Atanasovsko Lake, Bulgarian Black Sea coast (the first record of G. bubyri from the Black Sea...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4899-8
更新日期：2016-04-01 00:00:00
abstract：:Triatoma platensis is occasionally found coexisting with Triatoma infestans in chicken coops in Argentina. Some authors have reported the presence of hybrid specimens of both species in chicken coops and other peridomestic habitats. Given the coexistence of T. infestans with T. platensis and the possibility of generat...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06822-0
更新日期：2020-09-01 00:00:00
abstract：:Toxoplasma gondii is a zoonotic protozoan of almost all species of mammals and birds. However, no information is available about seroprevalence of the pathogen in stray dogs in seven cities from Inner Mongolia, Jilin, Liaoning province, China. Using the indirect hemagglutination antibody (IHA) test with a cutoff titer...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4746-y
更新日期：2015-12-01 00:00:00
abstract：:Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5166-3
更新日期：2016-10-01 00:00:00
abstract：:Wild birds are common hosts of ticks and can transport them for long distances, contributing to the spreading of tick-borne pathogens. The information about ticks on birds and tick-borne pathogens in Greece is limited. The present study aimed to evaluate the prevalence and species of ticks infesting wild resident bird...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4943-3
更新日期：2016-05-01 00:00:00
abstract：:This analysis investigated the influence of breed and gender on the pharmacokinetics of monepantel, and influence of breed, age, and gender on its efficacy against gastrointestinal nematodes of sheep. In a comparison of pharmacokinetic profiles from two studies, Merino lambs had significantly greater maximum concentra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1671-y
更新日期：2010-01-01 00:00:00
abstract：:Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 an...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3908-7
更新日期：2014-07-01 00:00:00
abstract：:This study aims to observe the histopathological alterations in schistosomula of Schistosoma japonicum induced by mefloquine. Mice were infected with S. japonicum cercariae, and after 14 days, a single dose of mefloquine (400 mg/kg) was administered orally. After 8 h, 24 h, 3 days, 7 days, and 14 days, groups of two t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1611-x
更新日期：2009-11-01 00:00:00
abstract：:Acetylcholinesterase (AChE) is a key enzyme involved in nerve impulse transmission in both vertebrates and invertebrates. In addition to neuromuscular AChE, many parasitic nematodes synthesize AChE in secretory glands and release the enzyme into their external environment. In this study, we evaluate the activities of ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3878-9
更新日期：2014-06-01 00:00:00
abstract：:This study aimed to identify tick species and to determine their relationship with the Theileria parva seroprevalence in cattle raised under an extensive farming system in North Kivu Province, Democratic Republic of Congo in two agro-ecological zones namely medium (1,000-1,850 m) and high (>1,850 m) altitude. Among th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3200-7
更新日期：2013-02-01 00:00:00
abstract：:Babesia motasi BQ1 (Lintan) was first isolated from Haemaphysalis qinghaiensis collected in Gannan Tibet Autonomous Region, Gansu province in April 2000. In this study, a total of 3,204 serum samples from small ruminants in 22 provinces located in different districts of China were tested for antibodies against merozoi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3310-x
更新日期：2013-06-01 00:00:00
abstract：:The Qinghai-Tibetan Plateau (QTP, in western China), which is the largest and highest plateau on Earth, is a highly epidemic region for Echinococcus spp. We collected 70 Echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern Qinghai and genotyped them using the mitochondr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2815-z
更新日期：2012-07-01 00:00:00
abstract：:A pellet freezing method, one type of rapid freezing protocol, was established for Trichomonas vaginalis. As cryoprotectants, 5-20% dimethylsulfoxide (DMSO), ethylene glycol, propylene glycol, and glycerol were used. Of these, DMSO showed a high protective effect, with a maximum effect at a concentration of 20%. No ef...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0559-y
更新日期：2007-09-01 00:00:00
abstract：:Catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including Schistosoma japonicum. S. japonicum tyrosine hydroxylase (SjTH) was amplified by rapid amplification of cDNA ends polymerase chain reaction that shows strong homo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2347-y
更新日期：2011-10-01 00:00:00
abstract：:The prevalence and abundance of infections with haemoparasites were studied over a 4-year period in Microtus arvalis (common vole, n =321) sampled from fallow grassland sites in north-eastern Poland. Total species richness was five (prevalence= Haemobartonella sp. 63.9%, Bartonella spp. 27.7%, Babesia microti 9.0%, Tr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-1040-1
更新日期：2004-03-01 00:00:00
abstract：:The activities of the disaccharidases lactase, maltase, sucrase and trehalase were examined in gerbils during Giardia lamblia infections. In a primary infection with trophozoites, the activities of all four enzymes were reduced from day 10 post-infection (p.i.) and remained at low levels well past the elimination phas...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932025
更新日期：1995-01-01 00:00:00
abstract：:This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0851-x
更新日期：2008-04-01 00:00:00
abstract：:In recent years, the mitochondrial haplotype diversity of the monogenean ectoparasites Gyrodactylus salaris Malmberg, 1957 on Atlantic salmon and G. thymalli Zitnan, 1960 on grayling has been studied intensively to understand the taxonomy and phylogeography of the two species. According to these studies, neither speci...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0423-5
更新日期：2007-05-01 00:00:00
abstract：:Infection in dogs and humans with the protozoan parasite Leishmania are widespread in tropical and subtropical countries around the globe. Sand flies of the order Phlebotomus in the Old World and Lutzomyia in the New World function as the vector of this disease. In dogs, skin lesions are the most prominent signs of ca...
journal_title：Parasitology research
pub_type： 临床试验,杂志文章
doi：10.1007/s00436-003-0905-7
更新日期：2003-07-01 00:00:00
abstract：:Tachyzoites of Toxoplasma gondii attach to the macrophage surface and are internalized either by a phagocytic process, which can be inhibited by cytochalasin D, or by an active process, independent of host cell actin. Previous studies have shown that parasite attachment induces the secretion of macromolecules found in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008500
更新日期：2000-01-01 00:00:00
abstract：:Our previous in vivo studies on angiostrongyliasis showed that PF1022A had stronger killing effects against female adults than against males. No killing effects were observed against young adult worms in the central nervous system. To characterize the former in vivo action of PF1022A, in vitro effects of PF1022A on th...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050301
更新日期：1997-01-01 00:00:00
abstract：:Understanding the fundamental factors influencing the epidemiology of wildlife disease is essential to determining the impact of disease on individual health and population dynamics. The host-pathogen-environment relationship of the endangered Australian sea lion (Neophoca cinerea) and the parasitic hookworm, Uncinari...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3997-3
更新日期：2014-09-01 00:00:00
abstract：:Tryptophan-N-formylated gramicidin A, a nonhemolytic derivative of the toxic peptide antibiotic gramicidin A, has previously been shown to induce potassium leakage from Plasmodium falciparum-infected erythrocytes in vitro and to inhibit the growth of the parasite. In the present study the antimalarial activities of tw...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932413
更新日期：1995-01-01 00:00:00
abstract：:Gossypol, a natural polyphenolic compound, induces growth-inhibitory and antiparasitic effects in Taenia taeniaeformis metacestodes in vivo and in vitro. We investigated the uptake and localization of [3H]-gossypol in this parasite. Metacestodes were incubated in 10(-5) M [3H]-gossypol at 37 degrees C. Parasites stead...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932182
更新日期：1993-01-01 00:00:00
abstract：:This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of P...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2514-1
更新日期：2012-02-01 00:00:00
abstract：:During our investigations on helminthofauna in roe deer (Capreolus capreolus) and red deer (Cervus elaphus) in France (1985-1998) we isolated nematodes not only of the genus Haemonchus Cobb, 1898 but also of the genus Ashworthius Le Roux 1930, both of which belong to the same subfamily of Haemonchinae. The prevalence ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008534
更新日期：2000-07-01 00:00:00
abstract：:A novel cDNA encoding a lysophospholipase (SmLPLH) homologue has been cloned from the human parasite, Schistosoma mansoni. The predicted primary structure of SmLPLH shares high sequence homology with mammalian lysophospholipases (62%) and also contains aconsensus signature peptide (GXSXG), which is present in all lipa...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050497
更新日期：1998-10-01 00:00:00
abstract：:Albendazole is known as the drug of choice for medical treatment of cystic echinococcosis (CE). Albendazole sulfoxide (ABZ-SO), as the main active metabolite of albendazole, has low efficacy in the disease due to low water solubility and poor absorptivity. PLGA nanoparticles (NPs) enhance the dissolution of poorly sol...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06901-2
更新日期：2020-12-01 00:00:00
