Abstract：
:The present investigation was undertaken to identify and characterize trophozoite proteases of five axenic strains of Giardia duodenalis isolated in Brazil and the reference strain Portland 1 isolated in the United States. Trophozoite cell lysates of each strain were analysed for the pattern of proteins and for proteolytic activity. Samples were tested in SDS-polyacrylamide gel electrophoresis for the protein profiles, and the detection of proteases in cell lysates was performed using substrate gel electrophoresis [gelatin, collagen, bovine serum albumin (BSA) and haemoglobin] and azocasein assays. Indeed, synthetic inhibitors were included in the assays to characterize the protease classes. Differences on the hydrolysis patterns of protein substrates were observed in relation to the substrate composition as much as the Giardia trophozoite strain. The substrate-containing gels revealed hydrolysis bands with molecular masses ranging from >97 to 20-15 kDa, and most zones were common to the five strains. However, some pronounced differences could be detected in the BTU-11 pattern. Azocasein was also degraded; however, depending on the lysate assayed, the degree of substrate degradation was variable. It was observed that inhibitory effects are substrate-dependent since the activity was predominantly due to cysteine proteases against gelatin, collagen, BSA and azocasein substrates and due to serine against haemoglobin. The presence of aspartic protease and aminopeptidase activity in the lysates was also indicated.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Coradi ST,Guimarães Sdoi
10.1007/s00436-005-0124-5subject
Has Abstractpub_date
2006-07-01 00:00:00pages
131-6issue
2eissn
0932-0113issn
1432-1955journal_volume
99pub_type
杂志文章abstract：:Mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. Dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. Recently, transmission has strongly increased in urban and semiurban areas, becoming a major international public ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4339-9
更新日期：2015-04-01 00:00:00
abstract：:Companion animals including dogs are one of the important components in One Health. Parasites may cause not only diseases in pet animals but also many zoonotic diseases infecting humans. In this study, we performed a survey of intestinal parasites in fecal specimens (n = 485) collected from outpatient pet dogs with di...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5631-7
更新日期：2018-01-01 00:00:00
abstract：:The purpose of this study was to evaluate morphologically the tongue of individuals with chronic Chagas disease (CD) in comparison to the non-chagasic ones. Twenty-four protocol cases of autopsies were selected. They were subdivided into CD patients (10 cases) and non-chagasic ones (14 cases). The morphometric analysi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0023-9
更新日期：2006-01-01 00:00:00
abstract：:A total of 480 snails were collected from 3 habitats on the Mau Escarpment, Kenya, and were identified as Bulinus tropicus. Of the 351 snails examined alive in London, 75 were infected with Calicophoron microbothrium, 39 with C. microbothrium and Schistosoma bovis, 1 with S. bovis, 24 with other species of trematodes ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931134
更新日期：1989-01-01 00:00:00
abstract：:The localization and distribution of SALMFamide (S1)-like immunoreactivity (IR), was determined at both the cellular and subcellular level in the central nervous system (CNS) of the nematode roundworm Ascaris suum. The techniques of indirect immunofluorescence in conjunction with confocal scanning laser microscopy and...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050086
更新日期：1996-01-01 00:00:00
abstract：:Black bullhead Ameiurus melas (Actinopterygii: Siluriformes) is an alien fish species of North American origin, which has expanded its invasive geographical range in Europe. In 2017-2019, 32 black bullhead specimens from the Lake Srebarna Biosphere Reserve, Bulgaria, were examined for monogenean parasites. Two species...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06699-z
更新日期：2020-07-01 00:00:00
abstract：:Schistosomiasis caused by different species of schistosome parasites is one of the most debilitating helminthic diseases of humans worldwide. For decades, chemotherapy is the main method of controlling schistosomiasis. However, the fear of drug resistance has motivated the search for alternatives. It has been demonstr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06224-x
更新日期：2019-03-01 00:00:00
abstract：:Praziquantel is still very effective for the treatment of schistosomiasis, but there are rising concerns on the potential risk of developing resistances because of the extensive use of this drug. Triclabendazole, a systemic anthelmintic, is very effective against other trematodes such as Paragonimus spp. and Fasciola ...
journal_title：Parasitology research
pub_type： 临床试验,杂志文章
doi：10.1007/s00436-007-0802-6
更新日期：2008-03-01 00:00:00
abstract：:Neodiplostomum americanum Chandler and Rausch, 1947 has been reported from six species of owls in North America. At present, there are no molecular data for this species and gene sequence data from Neodiplostomum Railliet, 1919 are limited. A freshly deceased specimen of the Great Horned Owl Bubo virginianus Gmelin, 1...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5503-1
更新日期：2017-08-01 00:00:00
abstract：:The purpose of the present study was to investigate the acaricidal and larvicidal activity against the larvae of Haemaphysalis bispinosa Neumann (Acarina: Ixodidae) and larvae of hematophagous fly Hippobosca maculata Leach (Diptera: Hippoboscidae) and against the fourth-instar larvae of malaria vector, Anopheles steph...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2473-6
更新日期：2012-08-01 00:00:00
abstract：:The fine structure of the infective hexacanths of Echinococcus multilocularis was examined with particular emphasis on the functional ultrastructure of penetration glands and nerve cells directly involved in the mechanism of initial host infection. The oncosphere contains two types of penetration glands, PG1 and PG2, ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5957-9
更新日期：2018-08-01 00:00:00
abstract：:Probolocoryphe species occur primarily as intestinal parasites of birds and mammals. Infection of the crab Nanosesarma minutum with the metacercarial cyst stages of Probolocoryphe uca is common in Kuwait Bay. In this study, the snail Cerithidea cingulata was used to determine if it would serve as first intermediate ho...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1778-1
更新日期：2010-04-01 00:00:00
abstract：:Strongyloides stercoralis can cause severe infection both in humans and dogs. Coproparasitological examination has low sensitivity for the diagnosis of this parasite; hence, different diagnostic techniques have been implemented. However, serology and molecular methods have been assessed almost exclusively in humans. I...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5468-0
更新日期：2017-07-01 00:00:00
abstract：:Cryptosporidium spp. are common intestinal protozoa causing diarrhea in humans and a variety of animal species. With the recent development of fur industry, a large number of fur animals are farmed worldwide, especially in China. The existence of identical Cryptosporidium species/genotypes in humans and fur animals su...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5686-5
更新日期：2018-01-01 00:00:00
abstract：:In this study, the larvicidal activity of an enriched fraction of the major lipophilic phenolic compounds from Hypericum carinatum Griseb. (Clusiaceae) was investigated against larvae of Aedes aegypti (Diptera: Culicidae), the main vector of dengue virus in Brazil. The larval mortality rate ranged 37.33 to 72.00 % at ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3401-8
更新日期：2013-06-01 00:00:00
abstract：:Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk of infection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes up-regulated in the schistosomulum stage in relation to ce...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2045-1
更新日期：2011-01-01 00:00:00
abstract：:In vitro leucocyte proliferative responses to parasite antigens and to mitogens as well as lymphocyte sub-types were compared in guinea pigs with genetically determined differences in their ability to express protective immunity against Trichostronylus colubriformis infection. Proliferative responses to parasite antig...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050048
更新日期：2000-04-01 00:00:00
abstract：:Little is known of the prevalence and genetic identity of Giardia duodenalis in sheep in Algeria. The present study aimed at characterizing G. duodenalis in lambs up to 6 months of age in Djelfa, Algeria. A total of 346 fecal specimens were collected from 28 farms and screened for G. duodenalis cysts by zinc sulfate f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06808-y
更新日期：2020-09-01 00:00:00
abstract：:Allicin is an active ingredient of garlic that has antibacterial, antifungal, antiviral, and antiprotozoal activity. However, the inhibitory effects of allicin on Babesia parasites have not yet been examined. In the present study, allicin was tested as a potent inhibitor against the in vitro growth of bovine and equin...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3654-2
更新日期：2014-01-01 00:00:00
abstract：:Plasmodium falciparum malaria in pregnancy is a major public health problem in sub-Saharan Africa and represents enormous diagnostic challenge. The objective of this report was to review scientific data from studies conducted in sub-Saharan Africa on the diagnosis of malaria in pregnancy within the last two decades (1...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0782-6
更新日期：2008-02-01 00:00:00
abstract：:Myiasis refers to the infestation of living humans and vertebrates with fly larvae that feed on necrotic or vital tissue of the host. Since the invasion of new fly species in Europe is currently being observed, which live obligatorily parasitically or are close relatives of such species, the aim of this study is to ob...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6145-7
更新日期：2019-02-01 00:00:00
abstract：:The volatile metabolites of Greek wild growing Thymus leucospermus and Thymus teucrioides subsp. candilicus were determined by gas chromatography and gas chromatography-mass spectrometry. The monoterpene hydrocarbon p-cymene (64.2%) dominated T. leucospermus oil, followed by γ-terpinene (7.9%), thymol (4.8%), and born...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2271-1
更新日期：2011-08-01 00:00:00
abstract：:The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0165-4
更新日期：2006-07-01 00:00:00
abstract：:We have recently demonstrated that the antimicrotubule drug oryzalin inhibits the growth of Entamoeba invadens as well as E. histolytica, the former being more resistant to the drug than the latter, and that effective doses of oryzalin are higher for Entamoeba than for the other parasitic protozoa examined thus far. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008542
更新日期：2000-08-01 00:00:00
abstract：:Giardia lamblia and Cryptosporidium parvum are recognized as the most common protozoan infections in Saudi Arabia. Microscopic examination of stool samples, either direct or concentrated, for the recovery of G. lamblia cysts and trophozoites and C. parvum oocysts is still the most commonly used for the diagnosis of bo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3319-1
更新日期：2013-04-01 00:00:00
abstract：:Hymenolepis diminuta is a zoonotic cestode parasitizing the small intestine of rodents (definitive hosts). Humans can accidentally enter into the life cycle of this tapeworm via the ingestion of infected insects (intermediate hosts) containing cestode cysticercoids in their body cavity. More than two centuries after t...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-020-06663-x
更新日期：2020-07-01 00:00:00
abstract：:In order to assess the potential of the stem bark of Kigelia africana (Lam.) Benth as source of new anti-malarial leads, n-hexane and ethyl acetate (EtOAc) extracts and four compounds isolated from the stem bark were screened in vitro against the chloroquine-resistant W-2 and two field isolates of Plasmodium falciparu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2363-y
更新日期：2011-06-01 00:00:00
abstract：:During investigations of fish parasites in the Neotropics (including the state of Veracruz and the Yucatán Peninsula in the Gulf of Mexico, the Chautengo Lagoon on the Pacific coast of the state of Guerrero in Mexico, and Lake Gatun in the Panama Canal), three monogenoidean (Dactylogyridae) species were found parasiti...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1442-9
更新日期：2009-09-01 00:00:00
abstract：:Argulus are common aquatic ectoparasites that create one of the major threats to aquaculture due to absence of suitable therapy. Piperine, a bioactive component of Piper longum, has medicinal properties and acts as anti-inflammatory, antibacterial, and antifungal, considering eco-friendliness and cost-effectiveness. T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3054-z
更新日期：2012-11-01 00:00:00
abstract：:Spermiogenesis and spermatozoon ultrastructure of the caryophyllidean cestode Breviscolex orientalis Kulakovskaya, 1962, first member of the family Capingentidae studied, a parasite of cyprinid fish Abbottina rivularis, are described using transmission electron microscopy. Spermiogenesis in B. orientalis follows the T...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2144-z
更新日期：2011-04-01 00:00:00
