Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens.


:We have recently demonstrated that the antimicrotubule drug oryzalin inhibits the growth of Entamoeba invadens as well as E. histolytica, the former being more resistant to the drug than the latter, and that effective doses of oryzalin are higher for Entamoeba than for the other parasitic protozoa examined thus far. The aim of the present study was to examine the effect of oryzalin on the encystation of E. invadens using an axenic encystation system in vitro. Oryzalin inhibited the encystation of E. invadens strain IP-1 in a dose-dependent manner. The addition of oryzalin after the induction of encystation was also inhibitory for encystation and cyst maturation. Trophozoites incubated for 1 day in encystation medium with oryzalin did not encyst after removal of the drug. Although trophozoites grown in the presence of 300 microM oryzalin for 2 days did not encyst after their transfer to encystation medium containing the same concentration of drug, a number of trophozoites survived for at least 3 days. In contrast, trophozoites grown in the absence of oryzalin neither survived nor encysted after their transfer to encystation medium supplemented with the drug, which suggests that pretreatment of trophozoites with oryzalin contributes to their continued survival as trophozoites, i.e., without their transforming into cysts, in encystation medium. Trophozoites grown with oryzalin did encyst after their transfer to encystation medium without the drug. Accumulation of trophozoites in the mitotic phase was observed after culture with oryzalin. When cysts prepared at day 1 of encystation, most of which were mononucleate, were reincubated in the presence of oryzalin for an additional 2 days, inhibition of their maturation was observed. Thus, oryzalin is a potent mitotic-phase inhibitor of E. invadens and may become a useful tool for studies on the relationship between the cell cycle and encystation of this parasite.


Parasitol Res


Parasitology research


Makioka A,Kumagai M,Ohtomo H,Kobayashi S,Takeuchi T




Has Abstract


2000-08-01 00:00:00












  • HLA class II alleles: susceptibility or resistance to cystic echinococcosis in Yemeni patients.

    abstract::This is the first study dealing with the association between HLA alleles and cystic echinococcosis (CE) in Yemeni patients. The present study aimed to detect the association of HLA-DRB(1) alleles and susceptibility or resistance to CE in Yemeni patients by HLA-DRB(1) typing; first by HLA-DRB(1) amplification using PCR...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Al-Ghoury AB,El-Hamshary EM,Azazy AA,Hussein EM,Rayan HZ

    更新日期:2010-07-01 00:00:00

  • The sources and metabolic dynamics of Schistosoma japonicum DNA in serum of the host.

    abstract::The polymerase chain reaction (PCR) assay has turned out to be one of the most potential tools for diagnosis of schistosomiasis. However, the source and metabolic dynamics of Schistosoma japonicum DNA in the blood of hosts is not clear. In this study, rabbit models with monosexual and mixed sexual cercariae infection ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Xu J,Liu AP,Guo JJ,Wang B,Qiu SJ,Sun H,Guan W,Zhu XQ,Xia CM,Wu ZD

    更新日期:2013-01-01 00:00:00

  • Epidemiology, pathophysiology, management and outcome of renal dysfunction associated with plasmodia infection.

    abstract::Malaria remains a serious health problem in many parts of the world. It causes high morbidity and claims many lives in developing countries each year. Humans are generally infected by four species of malaria parasites. However, malaria infection caused by Plasmodium malariae or P. falciparum is recognized as an import...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Elsheikha HM,Sheashaa HA

    更新日期:2007-10-01 00:00:00

  • Entomopathogenic marine actinomycetes as potential and low-cost biocontrol agents against bloodsucking arthropods.

    abstract::A novel approach to control strategies for integrated blood-feeding parasite management is in high demand, including the use of biological control agents. The present study aims to determine the efficacy of optimized crude extract of actinomycetes strain LK1 as biological control agent against the fourth-instar larvae...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Loganathan K,Kumar G,Kirthi AV,Rao KV,Rahuman AA

    更新日期:2013-11-01 00:00:00

  • New data on Neodiplostomum americanum Chandler and Rausch, 1947 (Digenea: Diplostomidae), in the Great Horned Owl Bubo virginianus Gmelin, 1788 and the Eastern Screech Owl Megascops asio Linnaeus, 1758 in Mississippi, USA.

    abstract::Neodiplostomum americanum Chandler and Rausch, 1947 has been reported from six species of owls in North America. At present, there are no molecular data for this species and gene sequence data from Neodiplostomum Railliet, 1919 are limited. A freshly deceased specimen of the Great Horned Owl Bubo virginianus Gmelin, 1...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Woodyard ET,Rosser TG,Griffin MJ

    更新日期:2017-08-01 00:00:00

  • Comparative studies on the proteomic expression patterns in the third- and fifth-stage larvae of Angiostrongylus cantonensis.

    abstract::Angiostrongylus cantonensis is an important zoonotic parasite causing eosinophilic meningitis and eosinophilic meningoencephalitis in humans. In this study, the protein expression profiles of the infective third- and pathogenic fifth-stage larvae (L3 and L5) of this parasite were compared by proteomic techniques. Isol...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Chen KY,Cheng CJ,Yen CM,Tang P,Wang LC

    更新日期:2014-10-01 00:00:00

  • Thecamoeba quadrilineata (Amoebozoa, Lobosa) as a new member of amphizoic amoebae-first isolation from endozoic conditions.

    abstract::A free-living soil amoeba Thecamoeba quadrilineata (Carter, 1856) Lepşi, 1960 (Amoebozoa: Thecamoebidae) was isolated from endozoic conditions for the first time. Presence of amoebae was detected after 4 days following inoculation of the gut of the earthworm Lumbricus terrestris on agar plate with Escherichia coli. On...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Borovičková T,Mrva M,Garajová M

    更新日期:2019-03-01 00:00:00

  • Supplementary studies and the first molecular data on Myxobolus scardinii Reuss, 1906 (Myxozoa: Myxosporea) infecting the gill filaments of rudd, Scardinius erythrophthalmus (L.).

    abstract::Within the present study, we offer new information on the morphology, histopathology, and 18S ribosomal DNA (rDNA) sequence of Myxobolus scardinii to supplement and revise the original description of Reuss (Bull Acad Imp Sci St-Pétersbourg, V Serie, XXV, No. 3: 199-205, 1906). M. scardinii was found infecting the gill...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Pekmezci GZ,Yardimci B,Yilmaz S

    更新日期:2015-10-01 00:00:00

  • Anisakis infection in allis shad, Alosa alosa (Linnaeus, 1758), and twaite shad, Alosa fallax (Lacépède, 1803), from Western Iberian Peninsula Rivers: zoonotic and ecological implications.

    abstract::Spawning individuals of allis shad, Alosa alosa (Linnaeus, 1758), and twaite shad, Alosa fallax (Lacépède, 1803), were sampled from three rivers on the Atlantic coast of the Iberian Peninsula (Ulla, Minho, Mondego) during 2008 to 2013 to assess the presence of the zoonotic marine parasite Anisakis spp. larvae. The res...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Bao M,Mota M,Nachón DJ,Antunes C,Cobo F,Garci ME,Pierce GJ,Pascual S

    更新日期:2015-06-01 00:00:00

  • Case report of systemic coccidiosis in a radiated tortoise (Geochelone radiata).

    abstract::More than 30 species of coccidian parasites have been described in Chelonidae (tortoises and turtles). Eimeria spp. are apparently the most common coccidia in chelonians. Findings of Caryospora cheloniae, Isospora sp., and Mantonella sp. have also been published, but reports about systemic coccidiosis are rare. We des...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Schmidt V,Dyachenko V,Aupperle H,Pees M,Krautwald-Junghanns ME,Daugschies A

    更新日期:2008-02-01 00:00:00

  • The ITS2 ribosomal DNA of Anopheles beklemishevi and further remarks on the phylogenetic relationships within the Anopheles maculipennis group of species (Diptera: Culicidae).

    abstract::Anopheles beklemishevi specimens from Russia were analysed by their ITS2 ribosomal DNA sequence to amend and to specify the phylogenetic tree of the Anopheles maculipennis species complex. Surprisingly, with 638 base pairs, the ITS2 regions of all the 34 An beklemishevi specimens examined were considerably longer than...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Kampen H

    更新日期:2005-09-01 00:00:00

  • Effect of glucantime on field and patient isolates of New World Leishmania: use of growth parameters of promastigotes to assess antimony susceptibility.

    abstract::The effect of pentavalent meglumine antimoniate (glucantime) on the growth kinetics of promastigotes of 13 South American Leishmania strains isolated from patients, sylvatic reservoirs, and vectors was studied. Four of five L. (Viannia) braziliensis human isolates were obtained from drug-responsive patients and one wa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Moreira ES,Anacleto C,Petrillo-Peixoto ML

    更新日期:1998-09-01 00:00:00

  • Adjuvant effect of ginsenoside-based nanoparticles (ginsomes) on the recombinant vaccine against Eimeria tenella in chickens.

    abstract::An experiment was conducted to study the adjuvant effect of ginsomes on the recombinant profilin in coccidian-infected breeding birds. Three-day-old chickens were vaccinated with Eimeria tenella recombinant profilin antigen (10, 50, and 100 μg per chicken) with or without 50 μg ginsomes per chicken. The boost vaccinat...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Zhang DF,Xu H,Sun BB,Li JQ,Zhou QJ,Zhang HL,Du AF

    更新日期:2012-06-01 00:00:00

  • Dose- and time-dependent functional and structural damage to the colon mucosa by Entamoeba histolytica trophozoite lysates.

    abstract::Analysis of the initial interaction between Entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lys...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Navarro-García F,López-Revilla R,Tstusumi V

    更新日期:1993-01-01 00:00:00

  • A survey for gregarines (Protozoa: Apicomplexa) in arthropods in Spain.

    abstract::Gregarines thrive in the digestive tract of arthropods and may be deleterious to their hosts, especially when present in high densities. The impact of parasites on these invertebrates may affect both the ecosystem equilibrium and human economic activities. However, information available on gregarines in Spain is limit...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Criado-Fornelio A,Verdú-Expósito C,Martin-Pérez T,Heredero-Bermejo I,Pérez-Serrano J,Guàrdia-Valle L,Panisello-Panisello M

    更新日期:2017-01-01 00:00:00

  • Feeding on different attractive flowering plants affects the energy reserves of Culex pipiens pallens adults.

    abstract::Mosquito adults usually need to ingest sugar from nectar host plants to sustain their metabolic needs. Mosquitoes could be differentially attracted by various flowering plant species, and the volatiles were thought to be important factors attributed to the differential attractiveness. However, whether mosquitoes' pref...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Yu BT,Hu Y,Ding YM,Tian JX,Mo JC

    更新日期:2018-01-01 00:00:00

  • Molecular characterization of the histone H2A gene from the parasite Trypanosoma rangeli.

    abstract::The sequence, genomic organization, and transcription of the gene encoding the H2A histone protein of the protozoan parasite Trypanosoma rangeli is described in this paper. The locus encoding the T. rangeli H2A protein is formed by at least 11 gene units measuring 790 nucleotides in length, organized in tandem, and lo...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Puerta C,Cuervo P,Thomas MC,Lopez MC

    更新日期:2000-11-01 00:00:00

  • Insecticide resistance and its molecular basis in urban insect pests.

    abstract::Insecticide resistance is one of the most important evolutionary phenomena for researchers. Overuse of chemicals has induced resistance in insect pests that ultimately has led to the collapse of disease control programs in many countries. The erroneous and inappropriate management of insect vectors has resulted in dis...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Naqqash MN,Gökçe A,Bakhsh A,Salim M

    更新日期:2016-04-01 00:00:00

  • Insights on the host associations and geographic distribution of Hymenolepis folkertsi (Cestoda: Hymenolepididae) among rodents across temperate latitudes of North America.

    abstract::Synoptic data and an understanding of helminth parasite diversity among diverse rodent assemblages across temperate latitudes of North America remain remarkably incomplete. Renewed attention to comprehensive survey and inventory to establish the structure of biodiverse faunas is essential in providing indicators and p...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Hoberg EP,Makarikov AA,Tkach VV,Meagher S,Nims TN,Eckerlin RP,Galbreath KE

    更新日期:2016-12-01 00:00:00

  • Amebic cysteine proteinase 2 (EhCP2) plays either a minor or no role in tissue damage in acute experimental amebic liver abscess in hamsters.

    abstract::Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic mi...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Olivos-García A,González-Canto A,López-Vancell R,García de León Mdel C,Tello E,Nequiz-Avendaño M,Montfort I,Pérez-Tamayo R

    更新日期:2003-06-01 00:00:00

  • Host-parasite relationship of Trypanosoma corvi in Ornithomyia avicularia.

    abstract::The first description of an electron microscopic study of Trypanosoma corvi in the vector Ornithomyia avicularia is reported. There is a close association between vector and parasite in the midgut, ileum and rectum of the vector. The midgut distribution of parasites is determined by the peritrophic membrane, which con...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Mungomba LM,Molyneux DH,Wallbanks KR

    更新日期:1989-01-01 00:00:00

  • Asian fish tapeworm, Khawia japonensis (Yamaguti, 1934), has expanded its European invasive range.

    abstract::The invasive fish tapeworm, Khawia japonensis (Yamaguti, 1934) originally described in Japan, is reported for the first time in Slovakia. The tapeworm was found in farmed common carp (Cyprinus carpio L.) from East-Slovakian breeding fish pond. This finding is registered 4 years after the first announcement of this par...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Oros M,Barčák D,Bazsalovicsová E,Hanzelová V

    更新日期:2015-05-01 00:00:00

  • Immunomodulation of liver injury by Ascaris suum extract in an experimental model of autoimmune hepatitis.

    abstract::Adult worm extract from Ascaris suum (Asc) has immunosuppressive activity and elicits Th2/IL-4/IL-10 response. This study evaluated the prophylactic and therapeutic effect of Asc in a murine model of concanavalin A (ConA)-induced autoimmune hepatitis (AIH). BALB/c mice received ConA, iv, (20 mg/kg), and three groups o...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Nascimento WC,Silva RP,Fernandes ES,Silva MC,Holanda GC,Santos PA,Albuquerque MP,Costa VA,Pontes-Filho NT,Souza VO

    更新日期:2014-09-01 00:00:00

  • Metazoan parasites and food composition of juvenile Etmopterus spinax (L., 1758) (Dalatiidae, Squaliformes) from the Norwegian Deep.

    abstract::A total of 37 juvenile Etmopterus spinax from the Norwegian Deep were examined for stomach contents and metazoan ecto- and endoparasites. These squaliform elasmobranchs were caught by benthopelagic net in May 2001 at a depth of 194-214 m. The euphausiid Meganyctiphanes norvegica and the teleost Maurolicus muelleri wer...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Klimpel S,Palm HW,Seehagen A

    更新日期:2003-03-01 00:00:00

  • Climate or host availability: what determines the seasonal abundance of ticks?

    abstract::Ticks can significantly affect the health and fitness of the host. Seasonal population dynamics of ticks play a vital role in disease transmission and the shaping of life-history traits of both tick and host. In this study, we examine the seasonal population dynamics of Ixodes hirsti in South Australia. For 2 years, w...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Oorebeek M,Kleindorfer S

    更新日期:2008-09-01 00:00:00

  • Are Teladorsagia circumcincta (Nematoda) morphs equally able to survive under anthelmintic treatment in sheep on pastures?

    abstract::The nematode Teladorsagia circumcincta is a polymorphic species, morphs T. c. trifurcata and T. c. circumcincta being the most frequent. Among other traits, T. c. trifurcata has lower infectivity than T. c. circumcincta. Anthelmintic treatments that regularly remove populations should favour the more infective T. c. c...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Leignel V,Cabaret J

    更新日期:2001-09-01 00:00:00

  • Ultrastructure of a Sarcocystis sp. infecting skinks of the genus Agama.

    abstract::Macroscopically visible sarcocysts were observed in the skeletal muscles of naturally infected skinks of the genus Agama (infection rate 11.3%). Sarcocysts were described by means of transmission electron microscopy. These cysts measured 0.03-0.25 x 0.38-1.7 mm (mean 0.12 x 1.1 mm). Typical mature cysts were bordered ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sakran TF

    更新日期:2000-09-01 00:00:00

  • The intraperitoneal inoculation of Lactobacillus casei in mice induces total protection against Trichinella spiralis infection at low challenge doses.

    abstract::The following effects of Lactobacillus casei in NIH mice were evaluated: the establishment of Trichinella spiralis adult worms in the intestine (AWI), larvae per gram of muscle tissue (LPG), levels of IgG and IgA, and levels of IL-4 and IFN-γ. One hundred and eight mice were allocated at random into 18 groups of six m...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Martínez-Gómez F,Fuentes-Castro BE,Bautista-Garfias CR

    更新日期:2011-12-01 00:00:00

  • Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequences.

    abstract::Seven isolates of Cryptocaryon irritans from different host species and geographical locations in Mainland China were characterized by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA) using two isolates of Ichthyophthirius multifiliis for comparative purposes. The...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sun HY,Zhu XQ,Xie MQ,Wu XY,Li AX,Lin RQ,Song HQ

    更新日期:2006-07-01 00:00:00

  • Sexual cycle of Babesia divergens confirmed by DNA measurements.

    abstract::The DNA content of the developmental stages within the life cycle of Babesia divergens was measured by means of fluorescence microscopy using the DNA-specific bisbenzimide Hoechst 33258. By comparing the mean relative fluorescence intensities, the assumption of sexual reproduction in the gut of the tick vector (Ixodes...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Mackenstedt U,Gauer M,Mehlhorn H,Schein E,Hauschild S

    更新日期:1990-01-01 00:00:00