Abstract：
:The recombinant surface protein of Theileria annulata (TaSP) was used in the standardization and validation of an enzyme linked immunosorbent assay (ELISA) for the detection of circulating antibodies against tropical theileriosis. ELISA data were expressed as the percentage positivity (PP) of the reactivity of an internal positive control. A total of 50 sera samples from a disease-free area were used for the calculation of the cut-off value which served as a threshold between the positive and the negative sera samples. This was determined as the mean PP plus two standard deviations or the twice the mean PP of the results obtained with these negative samples. The obtained thresholds were 17.8% and 18.3%, respectively. Accordingly, the reactivity of 140 field sera samples collected at random from an area known to be endemic for tropical theileriosis in Sudan was determined as PP values which were then compared to the results obtained using the indirect fluorescence antibody test (IFAT) from the same samples. Both tests showed a high degree of correlation. The TaSP-ELISA had a sensitivity of 99.1% and specificity of 90.47% when taking the IFAT as a reference test. Our test has proved its suitability for the diagnosis of tropical theileriosis and could be used in serological surveys to map out the prevalence of the disease or to monitor vaccination efficiencies in disease-free populations.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Bakheit MA,Schnittger L,Salih DA,Boguslawski K,Beyer D,Fadl M,Ahmed JSdoi
10.1007/s00436-003-1055-7subject
Has Abstractpub_date
2004-03-01 00:00:00pages
299-302issue
4eissn
0932-0113issn
1432-1955journal_volume
92pub_type
杂志文章abstract：:Gross anatomy of muscle and sensory/motor innervation of adult and intramolluscan developmental stages of Echinostoma caproni have been investigated to ascertain the organisation and the functional correlates of any stage-specific patterns of staining. Using indirect immunocytochemistry to demonstrate neuroactive subs...
journal_title：Parasitology research
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doi：10.1007/s00436-004-1120-x
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journal_title：Parasitology research
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journal_title：Parasitology research
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journal_title：Parasitology research
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journal_title：Parasitology research
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doi：10.1007/BF00930972
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journal_title：Parasitology research
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journal_title：Parasitology research
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更新日期：2012-07-01 00:00:00
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更新日期：2020-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s004360050405
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1702-8
更新日期：2010-02-01 00:00:00
