Abstract：
:An endopeptidase specific to the Plasmodium falciparum erythrocytic schizont stage and to free merozoites was detected using the fluorogenic GlcA-Val-Leu-Gly-Lys(or Arg)-AEC substrate. The enzyme was purified by high performance liquid chromatography (HPLC); its optimal activity was around pH 7.5 and its isoelectric point was 4.4. The molecular weight of the enzyme was about 68,000, as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. The endopeptidase was strongly inhibited by thiol proteinase inhibitors, leupeptin, and antipain. The possible involvement of this neutral endopeptidase in the reinvasion process is discussed.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Grellier P,Picard I,Bernard F,Mayer R,Heidrich HG,Monsigny M,Schrével Jdoi
10.1007/BF00930972subject
Has Abstractpub_date
1989-01-01 00:00:00pages
455-60issue
6eissn
0932-0113issn
1432-1955journal_volume
75pub_type
杂志文章abstract：:Supplementary feeding is widely used in game management but may aid the transmission of parasites. Firstly, feeding sites attract animals and may be regarded as high-risk areas for parasite transmission. Secondly, high host population densities resulting from and supported by supplementary feeding, as well as accumula...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5512-0
更新日期：2017-08-01 00:00:00
abstract：:After our promising results from prophylactic and therapeutic study (i.p. route) with the radio-attenuated Leishmania donovani parasites against experimental murine visceral leishmaniasis, we prompted to check their therapeutic efficacy through i.m route. BALB/c mice were infected with highly virulent L. donovani para...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2847-4
更新日期：2012-07-01 00:00:00
abstract：:Thus far, Entamoeba species have been classified based on morphology such as the number of nuclei in mature cysts and their hosts. Using recently developed molecular tools, ruminant Entamoeba spp. are currently classified into four species/genotypes: E. bovis and Entamoeba ribosomal lineages (RL) 1, 2, and 4. However,...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5689-2
更新日期：2018-01-01 00:00:00
abstract：:Biomphalaria glabrataembryonic (Bge) cells have been shown to provide favourable environmental conditions for the development of Schistosoma mansoni sporocysts. We investigated the effect of Bge excretory-secretory products on metabolic activity and gene transcription in S. mansoni mother sporocysts. Using the differe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0643-2
更新日期：2003-01-01 00:00:00
abstract：:Several murine monoclonal antibodies were raised against purified soluble Plasmodium falciparum antigens from the asexual blood stage. The monoclonal antibodies were purified from ascites by preparative agarose gel electrophoresis and tested for inhibitory activities against P. falciparum in vitro cultures. One monocl...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00535326
更新日期：1987-01-01 00:00:00
abstract：:Giardia is a prevalent intestinal parasitic infection. The trophozoite structural protein a1-giardin (a1-g) and the cyst protein cyst wall protein 2 (CWP2) have shown promise as Giardia vaccine antigen candidates in murine models. The present study assesses the genetic diversity of a1-g and CWP2 between and within ass...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5516-9
更新日期：2017-08-01 00:00:00
abstract：:Feasibility of implementing a DEC-fortified (DEC at 0.2% w/w and iodine) salt strategy to hasten elimination of diurnally sub-periodic Wuchereria bancrofti (DspWB) from the lone foci in Nancowry islands, Nicobar district, India, was assessed. This is a two-arm community-based study: one arm (12 villages, population 29...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06659-7
更新日期：2020-05-01 00:00:00
abstract：:In this paper, we cloned a novel full-length cDNA that encodes a Trichinella spiralis cathepsin B-like protease gene (TsCPB) using 3'-RACE PCR. The recombinant mature TsCPB protein (rTsCPB) was then expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3497-x
更新日期：2013-09-01 00:00:00
abstract：:Hepatozoon canis is a hemoprotozoan organism that infects domestic and wild carnivores throughout much of Europe. The parasite is mainly transmitted through the ingestion of infected ticks containing mature oocysts. The aims of the present survey were to determine the prevalence of H. canis in hunting dogs living in S...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06820-2
更新日期：2020-09-01 00:00:00
abstract：:In frozen sections of the acanthocephalan Pomphorhynchus laevis, which is a frequent intestinal parasite of cyprinid and salmonid fishes, leucine aminopeptidase (APase) was localized histochemically in outer parts of the presomal bulbus as well as in all layers and most nuclei of the metasomal body wall. Enzyme activi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932697
更新日期：1994-01-01 00:00:00
abstract：:A cross-sectional study was conducted to evaluate the seroprevalence of and risk factors for Toxoplasma gondii antibodies in 260 blood donors seen at blood banks in Mansoura University Hospital, Egypt. Blood donors were interviewed about sociodemographic characteristics and risk factors for T. gondii infection. A bloo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1350-z
更新日期：2009-06-01 00:00:00
abstract：:Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by Cryptosporidium parvum, Cryptosporidium meleagridis, and Cryptosporidium baileyi with zoonotic potential have also been reported in domestic birds, and recent studies indicate the presence of new host-adapted species or genotypes in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1810-5
更新日期：2010-05-01 00:00:00
abstract：:Plasmodium falciparum malaria in pregnancy is a major public health problem in sub-Saharan Africa and represents enormous diagnostic challenge. The objective of this report was to review scientific data from studies conducted in sub-Saharan Africa on the diagnosis of malaria in pregnancy within the last two decades (1...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-007-0782-6
更新日期：2008-02-01 00:00:00
abstract：:Tachyzoites of Toxoplasma gondii attach to the macrophage surface and are internalized either by a phagocytic process, which can be inhibited by cytochalasin D, or by an active process, independent of host cell actin. Previous studies have shown that parasite attachment induces the secretion of macromolecules found in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/pl00008500
更新日期：2000-01-01 00:00:00
abstract：:Leishmania (L.) tropica is a causative agent of cutaneous and occasionally visceral or viscerotropic leishmaniasis in humans. The dose of parasites influences the course and outcome of disease in some Leishmania species. The effect of parasite dose on L. tropica infection in an experimental model was studied in the cu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5991-7
更新日期：2018-09-01 00:00:00
abstract：:The insect growth regulator, Lufenuron, at concentrations of even multiples of LC(90) (determined under laboratory conditions) was tested against the III instar larvae of Aedes aegypti under simulated field conditions. For all concentrations tested, 100 % mortality of the larvae was observed within 24 h of exposure to...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2968-9
更新日期：2012-09-01 00:00:00
abstract：:Northern elephant seals (Mirounga angustirostris Gill, 1866), inhabiting rookeries on the mainland of Año Nuevo State Reserve in central California, were investigated in 2012 for presence of hookworms (Uncinaria spp.). Material collected and examined for hookworms included: blubber (n = 15), stomach and intestines (n ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2919-5
更新日期：2012-10-01 00:00:00
abstract：:Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050419
更新日期：1998-05-01 00:00:00
abstract：:A monoclonal antibody raised by immunization of BALB/c mice with erythrocytic stages of Plasmodium vivax was shown to react with asexual erythrocytic stages of P. chabaudi. The cross-reactivity molecules are antigens of 200 and 148 kDa in P. vivax and of 190 and 70 kDa in P. chabaudi. Immunofluorescence studies of the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050241
更新日期：1997-01-01 00:00:00
abstract：:When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1157-x
更新日期：2004-08-01 00:00:00
abstract：:A seasonal investigation on the occurrence of fishborne zoonotic trematodes (FZT) in economically important mono-cultured hybrid catfish and giant gouramy was conducted in the Mekong Delta of Vietnam. Fish from carp poly-culture and intensive small-scale integrated vegetable-aquaculture-animal husbandry farming (VAC) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0633-5
更新日期：2007-10-01 00:00:00
abstract：:Considering the snake venoms' pharmacological properties and chemotherapeutic potential as well as the need for new alternatives for Giardia infection treatment, the present study was carried out aiming to evaluate the in vitro effects of crude Crotalus durissus terrificus and Bothrops jararaca venoms on the growth an...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0037-3
更新日期：2006-03-01 00:00:00
abstract：:A key challenge in the successful treatment of Acanthamoeba infections is its ability to transform into a dormant cyst form that is resistant to physiological conditions and pharmacological therapies, resulting in recurrent infections. The carbohydrate linkage analysis of cyst walls of Acanthamoeba castellanii showed ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3254-6
更新日期：2013-03-01 00:00:00
abstract：:Reverse-transcriptase polymerase chain reaction cloning of the 3'-ends of the alpha-tubulin cDNAs of Tritrichomonas mobilensis in combination with Southern-blot analysis identified seven to eight distinct alpha-tubulin genes. All seven lack a carboxy-terminal tyrosine and the corresponding sequence compatible with pos...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050542
更新日期：1999-03-01 00:00:00
abstract：:The infectivity of Cryptosporidium muris (strain RN 66), originally isolated from the house rat (Iseki 1986), to various laboratory animals was studied by transmission experiments. After oral inoculation with 1 x 10(6) oocysts, mice, guinea pigs, rabbits, dogs, and cats all discharged endogenously produced oocysts in ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931279
更新日期：1989-01-01 00:00:00
abstract：:Synoptic data and an understanding of helminth parasite diversity among diverse rodent assemblages across temperate latitudes of North America remain remarkably incomplete. Renewed attention to comprehensive survey and inventory to establish the structure of biodiverse faunas is essential in providing indicators and p...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5255-3
更新日期：2016-12-01 00:00:00
abstract：:The aim of the study is to understand the anti-Clonorchis sinensis properties of mebendazole and albendazole, and compare to praziquantel and tribendimidine. Two hundred and thirty rats were divided into five batches for experimental treatment. In four batches, each rat was infected orally with 50 or 100 C. sinensis m...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2187-1
更新日期：2011-03-01 00:00:00
abstract：:A new nematode species, Philometra spicarae sp. n. (Philometridae), is described from a gravid female found in the abdominal cavity of spicarel, Spicara smaris (Linnaeus) (Centracanthidae, Perciformes), from the Ionian Sea off Sicily, Italy. The new species differs from most other Philometra spp. parasitic in the abdo...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-1877-z
更新日期：2010-07-01 00:00:00
abstract：:Precise identification of avian schistosomes in the genus Trichobilharzia at the species level is difficult and requires both traditional morphological and molecular techniques. To obtain satisfactory results by traditional methods, the characteristics of the intact adults or large fragments of male and females are ne...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06573-7
更新日期：2020-03-01 00:00:00
abstract：:The parasite community structure of the neotropical cormorant, Phalacrocorax brasilianus, from two lagoons (Coyuca and Tres Palos) from Guerrero state, México, was examined. Fourteen species of adult helminths (6,391 individuals) from 48 cormorants were identified: 9 digeneans, 1 acanthocephalan, 1 cestode, and 3 nema...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2377-5
更新日期：2011-11-01 00:00:00
