Abstract:
:SAG2 locus, the coding gene of the P22 protein, has been widely used for the molecular epidemiology of Toxoplasma gondii and characterization of the parasite isolates with two separate polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) processes. To re-assess the resolution power and suitability of this genetic marker for molecular characterization of the parasite isolates, a number of 27 Toxoplasma strains from different zymodeme patterns were used in the present study. Both codon and non-codon regions of the SAG2 locus of all 27 strains were amplified and subjected to sequencing and nucleotide alignment. Nucleotide variations clustered the three major genotypes (I, II and III). Some minor genotypes, unidentifiable by SAG2-RFLP, could be identified by sequence comparison. However, there were other genotypes that could not be differentiated from the major types due to having identical sequences. This suggests that a remarkable number of field isolates representing several minor types will be miss-clustered with the major types by using the traditional SAG2-PCR-RFLP method. It was concluded that this technique seems not to be suitable for Toxoplasma population study. Thus, the utilization of more variable markers and other discriminatory methods are also recommended.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Fazaeli A,Ebrahimzadeh Adoi
10.1007/s00436-006-0449-8subject
Has Abstractpub_date
2007-06-01 00:00:00pages
99-104issue
1eissn
0932-0113issn
1432-1955journal_volume
101pub_type
杂志文章abstract::The tegument proteins of schistosomes are regarded as potential vaccine candidates and drug targets to control schistosomiasis. Nucleotide pyrophosphatase/phosphodiesterase-5 (NPP-5), which belongs to a multigene family of nucleotide pyrophosphatase/phosphodiesterases (NPPs), is important in the hydrolysis of pyrophos...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2552-8
更新日期:2012-02-01 00:00:00
abstract::In the present study, we sequenced and analyzed the mitochondrial (mt) genome of Gastrothylax crumenifer and compared it with other selected trematodes. The full mt genome of G. crumenifer was amplified, sequenced, assembled, analyzed and then subjected to phylogenetic analysis. The complete mt genome of G. crumenifer...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5019-0
更新日期:2016-06-01 00:00:00
abstract::Avian malaria is of significant ecological importance and serves as a model system to study broad patterns of host switching and host specificity. The erythrocyte invasion mechanism of the malaria parasite Plasmodium is mediated, in large part, by proteins of the erythrocyte-binding-like (ebl) family of genes. However...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-3211-4
更新日期:2013-03-01 00:00:00
abstract::Attractive toxic sugar bait (active ingredient, 1% boric acid) was evaluated against Aedes albopictus Skuse populations in the laboratory, semi-field trials, and field trials in residential communities in St. Augustine, Florida. Laboratory evaluations of boric acid sugar baits applied to the plant Pentas lanceolata (R...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3312-8
更新日期:2013-04-01 00:00:00
abstract::Differentiation between viable and non-viable hookworm ova in environmental samples is necessary in order to implement strategies to mitigate re-infections in endemic regions. In this study, an untargeted metabolic profiling method was developed that utilised gas chromatography-mass spectrometry (GC-MS) in order to in...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5112-4
更新日期:2016-09-01 00:00:00
abstract::Cryptosporidiosis, caused by Cryptosporidium, causes self-limited diarrhea in normal hosts but may cause life-threatening diarrhea in immunocompromised persons. Cryptosporidium-induced manifestations, including weight-loss and intestinal physiological alterations are not noted in adult immunocompetent mice. So far, st...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0785-3
更新日期:2008-02-01 00:00:00
abstract::Seventy perch (Perca fluviatilis) caught in Lake Constance at Langenargen in May 1990 were examined for parasites. The frequency distributions of the eyefluke Diplostomum spathaceum (Rudolphi, 1819), and the tapeworms Proteocephalus percae (Müller, 1780) and Triaenophorus nodulosus (Pallas, 1781) fitted closely to a n...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050264
更新日期:1997-01-01 00:00:00
abstract::Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with st...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1138-6
更新日期:2008-11-01 00:00:00
abstract::During their different life stages, parasites undergo remarkable morphological, physiological, and behavioral "metamorphoses" to meet the needs of their changing habitats. This is even true for ectoparasites, such as the monogeneans, which typically have a free-swimming larval stage (oncomiracidium) that seeks out and...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-07045-z
更新日期:2021-01-12 00:00:00
abstract::Sheep infected with the triclabendazole-susceptible Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48, and 72 h post-treatment (p.t.). Changes to the spermatogenic cells in the testis were examined by...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1649-9
更新日期:2010-01-01 00:00:00
abstract::Different Tunga penetrans isolates from various hosts obtained from South America (Fortaleza. Brazil) have been studied by nucleotide sequence comparison of the first and the second internal transcribed spacer (ITS1, ITS2) of the ribosomal deoxyribonucleic acid (rDNA) and part of the mitochondrial 16S rDNA. Results sh...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0743-0
更新日期:2008-01-01 00:00:00
abstract::Among tick species, members of the subfamily Amblyomminae have received special attention, since they serve as vectors for pathogens such as Rickettsia spp. and display cryptic species complexes that make their taxonomical classification challenging. Amblyomma ovale, Amblyomma maculatum, and other species of the genus...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5592-x
更新日期:2017-11-01 00:00:00
abstract::The bacteria Anaplasma platys, Anaplasma phagocytophilum and Ehrlichia canis are tick-borne agents that cause canine vector-borne disease. The prevalence of these pathogens in South Eastern Europe is unknown with the exception of an isolated case of A. platys detected in a dog imported into Germany from Croatia. To ga...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5611-y
更新日期:2017-11-01 00:00:00
abstract::Within the present study, we offer new information on the morphology, histopathology, and 18S ribosomal DNA (rDNA) sequence of Myxobolus scardinii to supplement and revise the original description of Reuss (Bull Acad Imp Sci St-Pétersbourg, V Serie, XXV, No. 3: 199-205, 1906). M. scardinii was found infecting the gill...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4587-8
更新日期:2015-10-01 00:00:00
abstract::The physicochemical properties of four N-halomethylated and one non-halomethylated ammonium salts, with proven in vitro antileishmanial activity, were determined according to pharmaceutical standard procedures. The effectiveness and toxicity of these compounds were assessed in hamsters infected with Leishmania (Vianni...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5702-9
更新日期:2018-01-01 00:00:00
abstract::Giardia lamblia and Cryptosporidium parvum are recognized as the most common protozoan infections in Saudi Arabia. Microscopic examination of stool samples, either direct or concentrated, for the recovery of G. lamblia cysts and trophozoites and C. parvum oocysts is still the most commonly used for the diagnosis of bo...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3319-1
更新日期:2013-04-01 00:00:00
abstract::A molecular approach was used to analyse a focus of cercarial dermatitis in northeastern France (Lake Der-Chantecoq), including both cercariae and snails,by sequencing the internal transcribed spacers (ITS1 for ocellate furcocercariae and ITS2 for snails). Lymnaea stagnalis were found infected with the furcocercariae ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1273-7
更新日期:2005-01-01 00:00:00
abstract::The endemic avifauna of Arabia is unique and characteristic through the whole region. Little is known about these birds and their ectoparasites. The Arabian partridge Alectoris melanocephala (Rüppell, 1835) and Philby's partridge Alectoris philbyi Lowe, 1934 are two endemic species which are distributed through the Sa...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5219-7
更新日期:2016-11-01 00:00:00
abstract::The Stomacher is very widely used in food and medical research for extracting tissues. To determine whether nematode larvae were disrupted by the Stomacher, L3 larvae of Haemonchus contortus were homogenised for up to 40 min at full power but no larval disruption occurred. Therefore, tissue from the mucosa and submuco...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1473-2
更新日期:2009-08-01 00:00:00
abstract::In frozen sections of the acanthocephalan Pomphorhynchus laevis, which is a frequent intestinal parasite of cyprinid and salmonid fishes, leucine aminopeptidase (APase) was localized histochemically in outer parts of the presomal bulbus as well as in all layers and most nuclei of the metasomal body wall. Enzyme activi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00932697
更新日期:1994-01-01 00:00:00
abstract::The widespread apicomplexan parasites Toxoplasma gondii (T. gondii) and Eimeria tenella (E. tenella) are important pathogens with high prevalence in poultry. The aim of our study was the investigation of mutual influences in co-infected chickens, focusing on immune response and course of infection. Two separate trials...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5636-2
更新日期:2017-11-01 00:00:00
abstract::The effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro application was done...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5056-8
更新日期:2016-08-01 00:00:00
abstract::The artemisinin-based combination therapy (ACT) is adopted by several countries as first line for malaria treatment in the last decade. Concomitantly, the World Health Organization and other research reports showed a dramatic decline in malaria burden in terms of morbidity, mortality and treatment failure (TF). The op...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-009-1700-x
更新日期:2010-02-01 00:00:00
abstract::With the advent of polymerase chain reaction (PCR), genetic markers are now accessible for all organisms, including parasites. Amplified fragment length polymorphism (AFLP) is a PCR-based marker for the rapid screening of genetic diversity and intraspecific variation. It is a potent fingerprinting technique for genomi...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-012-3238-6
更新日期:2013-02-01 00:00:00
abstract::Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by C. meleagridis or C. baileyi with zoonotic potential have also been reported in domestic birds; and recent studies indicate the presence of new host-adapted species or genotype in birds. Therefore, accurately identifying isolates is...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1082-z
更新日期:2004-04-01 00:00:00
abstract::The discovery and optimization of antiparasitic compounds has profited by information-based methods newly emerged in the modern drug development process. The generation of computer models enables the cost-efficient and fast computational screening of virtual compound libraries for biologically active molecules. Two so...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-002-0773-6
更新日期:2003-06-01 00:00:00
abstract::Seventy-five previously frozen Northern Lapwings, Vanellus vanellus (Linnaeus, 1758) were dissected and examined for trematodes. The available birds were euthanized after suffering severe injuries from a hail storm. Trematode specimens were found in the air sacs, body cavities and kidneys. They were cleared, stained i...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-5330-9
更新日期:2017-02-01 00:00:00
abstract::Balantidium coli is a ciliated protozoon inhabiting the colon of swine, rodents, horses, nonhuman primates and humans. In association with disease triggered by other infectious agents, B. coli may become a pathogenic opportunist. This study describes the isolation, cultivation, morphological as well as molecular chara...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1673-9
更新日期:2010-01-01 00:00:00
abstract::A total of 37 juvenile Etmopterus spinax from the Norwegian Deep were examined for stomach contents and metazoan ecto- and endoparasites. These squaliform elasmobranchs were caught by benthopelagic net in May 2001 at a depth of 194-214 m. The euphausiid Meganyctiphanes norvegica and the teleost Maurolicus muelleri wer...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-002-0741-1
更新日期:2003-03-01 00:00:00
abstract::Numerous nematodes were found in the rectum of three fish species Synodontis ocellifer, S. nigrita, and S. schall (Mochokidae, Siluriformes) from the Gambia River and Mare Simenti, National Park Niokolo Koba, Senegal. A nematode species Raillietnema synodontisi Vassiliadès, 1973 (host S. ocellifer), is redescribed usi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0869-0
更新日期:2008-04-01 00:00:00