Abstract：
:Cryptosporidiosis, caused by Cryptosporidium, causes self-limited diarrhea in normal hosts but may cause life-threatening diarrhea in immunocompromised persons. Cryptosporidium-induced manifestations, including weight-loss and intestinal physiological alterations are not noted in adult immunocompetent mice. So far, studies that have been used to test the therapeutic efficacy of drugs have been performed using various immunocompromised animal models. There is an urgent need of an immunocompetent small animal model that portrays Cryptosporidium-induced manifestations. In the current studies, we have compared two Cryptosporidium parvum pretreatment methods, we have hence used sodium hypochlorite or acidic water to treat Cryptosporidium parvum, followed by infection by oral gavage in adult immunocompetent C57BL6 mice. We demonstrated manifestations such as weight loss, intestinal structural and physiological alterations such as intestinal, villi blunting, and glucose malabsorption (as studied by the Ussing chamber technique) only in response to infection with C. parvum that has been treated with acidic water and not with sodium hypochlorite. These novel studies reveal that acidic water treatment of C. parvum results in manifestations of cryptosporidiosis in otherwise resistant immunocompetent mice. The current studies open up possibilities of using the normal immunocompetent mice model to test therapeutic drugs against cryptosporidiosis.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Garza A,Castellanos-Gonzalez A,Griffiths J,Robinson Pdoi
10.1007/s00436-007-0785-3subject
Has Abstractpub_date
2008-02-01 00:00:00pages
457-63issue
3eissn
0932-0113issn
1432-1955journal_volume
102pub_type
杂志文章abstract：:Sheared flagella from Giardia lamblia were freed from cytoskeleton fragments and other cell contaminants by centrifuging in a density gradient. The purified organelles contain many polypeptides, including a set of low-molecular-weight antigens [apparent molecular weights (MWs) = 31, 32, 34, 35 and 37 kD] in the same s...
journal_title：Parasitology research
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journal_title：Parasitology research
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journal_title：Parasitology research
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pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00935425
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050287
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2000-02-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2593-z
更新日期：2012-03-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2776-7
更新日期：2012-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5035-0
更新日期：2016-07-01 00:00:00
abstract：:The aim of this paper was to investigate the component communities of parasites in Chaetobranchopsis orbicularis from a tributary of the Amazon River system, in Northern Brazil. In 32 fish examined, 902,551 parasites were collected, including Ichthyophthirius multifiliis, Piscinoodinium pillulare, Sciadicleithrum geop...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
