Abstract:
:Balantidium coli is a ciliated protozoon inhabiting the colon of swine, rodents, horses, nonhuman primates and humans. In association with disease triggered by other infectious agents, B. coli may become a pathogenic opportunist. This study describes the isolation, cultivation, morphological as well as molecular characterization of B. coli isolated from the large intestine of a pig in the Philippines. Based on scanning and transmission electron microscopy, this protozoon presents a dense ciliation in the oral structure and somatic cilia that are arranged in a more transverse field. Oral and somatic monokinetids were identified in the cortex of the organism. The presence of heterokaryotic nuclear condition is evident, and the cell body of the ciliate shows numerous mucocysts, several food vacuoles, mitochondria, endoplasmic reticulum, and contractile vacuoles. Polymerase chain reaction and phylogenetic analysis based on the small subunit ribosomal RNA gene were performed in order to compare our isolate with other previously reported B. coli isolates. The full-length sequence of the SSU rRNA gene of the isolate showed 99% similarity to other B. coli isolates reported in the GenBank. Phylogenetic analysis revealed that the isolate clustered with previously reported B. coli isolates from gorillas, pig, and ostrich. To date, no studies on the ultrastructure and phylogeny of B. coli isolated in the Philippines have been reported. Results from this study may serve as a baseline data for further ultrastructural and phylogenetic studies on this organism. This study also suggests that morphological characteristics along with molecular identification are essential for validating and identifying species of Balantidium.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Nilles-Bije ML,Rivera WLdoi
10.1007/s00436-009-1673-9subject
Has Abstractpub_date
2010-01-01 00:00:00pages
387-94issue
2eissn
0932-0113issn
1432-1955journal_volume
106pub_type
杂志文章abstract::Ultrastructural changes of gastritis due to infection with Physaloptera clausa in 12 fresh carcasses of euthanized European hedgehogs (Erinaceus europaeus) collected from different part of Urmia, Iran, in which they were highly populated with this animal, six females and six males were subjected to detail necropsy wit...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4597-6
更新日期:2015-10-01 00:00:00
abstract::Myxobolus miyairii Kudo, 1919 was first reported from the intestines of the Amur catfish (Silurus asotus) in Japan and then in China and Russia, but with incomplete description. During the investigation of fish myxosporean diversity in Poyang Lake, the biggest lake along the Yangtze River, China, two Amur catfish high...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4889-x
更新日期:2016-04-01 00:00:00
abstract::Exposure to viable Acanthamoeba may cause fatal encephalitis and blinding keratitis in humans. Quantification of environmental Acanthamoeba by a reliable analytical assay is essential to assess the risk of human exposure and efficacy of control measures (e.g., superheating). Two DNA binding dyes (ethidium monoazide (E...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3556-3
更新日期:2013-11-01 00:00:00
abstract::In Egypt, reclaiming portions of the desert using water from the Nile has resulted in large-scale invasion of Biomphalaria alexandrina in these regions. Studies exploring the local adaptation of Schistosoma mansoni to its snail host have been carried out to predict the extension of schistosomiasis to newly reclaimed a...
journal_title:Parasitology research
pub_type: 杂志文章,meta分析
doi:10.1007/s00436-014-3756-5
更新日期:2014-03-01 00:00:00
abstract::Limited data is available on the vertical transmission of Neospora caninum via the colostrum. The results of our previous research revealed the presence of N. caninum DNA in the milk of seropositive cows. The aim of the present work is to demonstrate parasite DNA in colostrum samples. A polymerase chain reaction using...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0288-7
更新日期:2007-02-01 00:00:00
abstract::Spirometra erinacei is a pseudophyllidean tapeworm which inhabits the intestines of cats and dogs. The infections are usually asymptomatic in these animals, but the infection of the plerocercoid larvae of the parasite, spargana, cause sparganosis in other vertebrates, including human. In this study, we identified a ge...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1714-4
更新日期:2010-02-01 00:00:00
abstract::Many factors contribute to parasites varying in host specificity and distribution among potential hosts. Metagonimoides oregonensis is a digenetic trematode that uses stream-dwelling plethodontid salamanders as second intermediate hosts in the Eastern US. We completed a field survey to identify which stream salamander...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4550-8
更新日期:2015-08-01 00:00:00
abstract::The present paper reports the ability of egg-propagated gametocyte vaccine to induce protective immunity against Eimeria tenella infections in offspring chicks. For this purpose, breeding hens were immunized with adjuvanted gametocyte vaccine orally, being the best vaccine in our previous studies. Eggs collected 2 wee...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-006-0382-x
更新日期:2007-04-01 00:00:00
abstract::Ultrastructural characters of the spermiogenesis and mature spermatozoon of Notopentorchis sp. (Cestoda, Cyclophyllidea, Paruterinidae), a parasite from Apus affinis (Aves, Apodiformes, Apodidae) from Gabon, are described by means of transmission electron microscopy. Cytochemical analysis for detection of glycogen was...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2809-2
更新日期:2012-07-01 00:00:00
abstract::Chagas disease, caused by the protozoan Trypanosoma cruzi, remains a serious public health problem in Latin America. In relation to digestive problems, 4.5% of patients show mega syndromes (megacolon) in the chronic phase. In this article, we evaluated intestinal motility at the acute phase of T. cruzi infection throu...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0829-8
更新日期:2008-03-01 00:00:00
abstract::Adult liver flukes belonging to three isolates of differing sensitivity to triclabendazole were incubated for 24 h in vitro in nitroxynil at a concentration of 100 microg/ml. Fine structural changes to the tegument, sub-tegumental region and gut were assessed by transmission electron microscopy. Similar changes were o...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1241-8
更新日期:2009-02-01 00:00:00
abstract::The purpose of the present study was to assess the effect of crude extracts of marine actinobacteria on larvicidal, repellent, and ovicidal activities against Culex tritaeniorhynchus and Culex gelidus (Diptera: Culicidae). The early fourth instar larvae of C. tritaeniorhynchus and C. gelidus, reared in the laboratory,...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2193-3
更新日期:2011-06-01 00:00:00
abstract::The present study represents the first detailed description of the haptoral sclerites of Macrogyrodactylus clarii Gussev 1961. Light microscopy reveals outgrowths of the hamuli roots; two lateral spine-like extensions of the hood-like accessory sclerites; a ridged, fan-shaped distal end of an accessory sclerite; and t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06799-w
更新日期:2020-12-01 00:00:00
abstract::Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. A complete open reading frame of oligopeptidase B from Leishmania amazonensis was amplified with polymerase chain reaction with gradient annealing temperatures using primers designed...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0552-5
更新日期:2007-09-01 00:00:00
abstract::Sera of ten dogs infected with Angiostrongylus vasorum were analyzed by immunoblotting proteins of first stage larvae (L1) and adult parasites. The molecular weights (m.w.) of the principal L1 proteins identified by IgG, IgM, IgA, and IgE were 18-118 kDa and those of the adult parasite were 28-209 kDa. The L1 proteins...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0774-6
更新日期:2008-02-01 00:00:00
abstract::The pathogenicity of 19 hypocrealean entomopathogenic fungi from seven different genera in adult Aedes aegypti was tested. All fungi proved to be pathogenic, and Isaria fumosorosea, Lecanicillium muscarium, Lecanicillium psalliotae, Metarhizium anisopliae, Metarhizium lepidiotae, Metarhizium majus, Metarhizium frigidu...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1991-y
更新日期:2010-10-01 00:00:00
abstract::Blastocytis sp. is the most common enteric protozoan in human, but its pathogenesis is still unclear. To study the infectious effects of Blastocytis sp. on tissue damage, we orally challenged the Sprague Dawley (SD) rats with different doses of Blastocytis subtype1 (ST1) and examined the histological changes. We found...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3536-7
更新日期:2013-10-01 00:00:00
abstract::Two tracer tests were conducted between August 2004 and March 2007 at an ecological farm in western Bohemia. The first tracer test was performed for the summer-autumn grazing period (onset of arrested development), the second for spring (resumption of arrested development). In the first tracer test, the percentage of ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-0984-6
更新日期:2008-07-01 00:00:00
abstract::The purpose of the present study was to investigate the acaricidal and larvicidal activity against the larvae of Haemaphysalis bispinosa Neumann (Acarina: Ixodidae) and larvae of hematophagous fly Hippobosca maculata Leach (Diptera: Hippoboscidae) and against the fourth-instar larvae of malaria vector, Anopheles steph...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2473-6
更新日期:2012-08-01 00:00:00
abstract::The metacercarial cyst walls of Paragonimus westermani, P. miyazakii, P. ohirai, and P. iloktsuenensis were examined using ultrastructural and histochemical techniques. The cyst walls of P. westermani, P. miyazakii, and P. ohirai were found to have five distinct layers, whereas the wall of P. iloktsuenensis had only t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931563
更新日期:1992-01-01 00:00:00
abstract::Entamoeba histolytica infection in humans is still one of the leading causes of parasitic diseases among the developing countries, including India. It therefore becomes extremely important to characterize this parasite with the aim of preventing and controlling the amoebiasis which it causes. The present study describ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360000363
更新日期:2002-02-01 00:00:00
abstract::The present work was aimed at evaluating the "in vitro" efficacy of different concentrations of thymol on engorged nymphs and females of Rhipicephalus sanguineus. The nymphs were separated in seven groups and immersed in different concentrations of thymol (0.25%, 0.5%, 1.0%, 1.5%, and 2.0%) for 5 min. A control group ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1530-x
更新日期:2009-10-01 00:00:00
abstract::The distribution of actin filaments in the spermatogenic cells of Fasciola hepatica was determined using a fluorescent derivative of phalloidin. Actin was localised primarily in the region of separation of a secondary spermatogonium from a primary spermatogonium, in the inner faces at the centre of four-cell clusters ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00935425
更新日期:1991-01-01 00:00:00
abstract::We examine the taxonomic composition and endemism of adult helminth parasites of freshwater fishes of Mexico, with regard to the main hydrological basins of the country. A presence-absence matrix, including every species of adult helminth parasites of freshwater fishes from 23 Mexican hydrological basins was compiled ...
journal_title:Parasitology research
pub_type: 杂志文章,评审
doi:10.1007/s00436-012-3175-4
更新日期:2013-01-01 00:00:00
abstract::Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF02351874
更新日期:1994-01-01 00:00:00
abstract::Three actinosporean types--raabeia, aurantiactinomyxon, and guyenotia--from the oligochaete Branchiura sowerbyi Beddard collected from a crucian carp (Carassius auratus gibelio) pond are described in this report. Compared with the actinospores described previously, the raabeia type presents similar spore shape with th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3306-6
更新日期:2013-04-01 00:00:00
abstract::Angiostrongylus vasorum is a metastrongyloid nematode that may cause cardiopulmonary disease, neurological signs and coagulopathies in dogs. The parasite has an indirect life cycle with molluscs as intermediate hosts, in which the infective third larval stage develops. Recently, A. vasorum has been repeatedly reported...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3532-y
更新日期:2013-10-01 00:00:00
abstract::The aims of this study are to identify Leishmania species and compare and validate internal transcribed spacers (ITS) polymerase chain reaction (PCR) assay against parasitological methods for diagnosing cutaneous leishmaniasis (CL). We used the ITS-PCR, parasite culture, and microscopic evaluation of stained smears on...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1111-4
更新日期:2008-10-01 00:00:00
abstract::The time-course of the detection of circulating and urinary filarial antigens was followed with a 2S-IRMA assay, using a mouse monoclonal antibody raised against Brugia malayi larvae, in Mastomys natalensis experimentally infected with Brugia malayi, Brugia pahangi, or Litomosoides carinii. In the prepatent phase of t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00536033
更新日期:1987-01-01 00:00:00
abstract::The aim of the study is to understand the anti-Clonorchis sinensis properties of mebendazole and albendazole, and compare to praziquantel and tribendimidine. Two hundred and thirty rats were divided into five batches for experimental treatment. In four batches, each rat was infected orally with 50 or 100 C. sinensis m...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2187-1
更新日期:2011-03-01 00:00:00