Role of surface components in the process of interaction of Giardia duodenalis with epithelial cells in vitro.

Abstract:

:Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment of the parasites to the epithelial cells. Experiments in which the parasites or epithelial cells were treated before interaction showed that these monosaccharides interfered with both cell surfaces. Trypsin-sensitive, but not neuraminidase-sensitive, groups exposed on the cell surface are important for the parasite-epithelial cell association. Fluorescein isothiocyanate (FITC)- or colloidal gold-labeled lectins were used to analyse the distribution of carbohydrates on the surface of G. duodenalis and epithelial cells. It is important to stress here the presence of fucose on the parasite surface. Treatment of the cells with lectins was also used to analyse the role of carbohydrate-containing macromolecules in the parasite-cell interaction.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Pegado MG,de Souza W

doi

10.1007/BF02351874

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

320-6

issue

4

eissn

0932-0113

issn

1432-1955

journal_volume

80

pub_type

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