Abstract:
:Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment of the parasites to the epithelial cells. Experiments in which the parasites or epithelial cells were treated before interaction showed that these monosaccharides interfered with both cell surfaces. Trypsin-sensitive, but not neuraminidase-sensitive, groups exposed on the cell surface are important for the parasite-epithelial cell association. Fluorescein isothiocyanate (FITC)- or colloidal gold-labeled lectins were used to analyse the distribution of carbohydrates on the surface of G. duodenalis and epithelial cells. It is important to stress here the presence of fucose on the parasite surface. Treatment of the cells with lectins was also used to analyse the role of carbohydrate-containing macromolecules in the parasite-cell interaction.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Pegado MG,de Souza Wdoi
10.1007/BF02351874subject
Has Abstractpub_date
1994-01-01 00:00:00pages
320-6issue
4eissn
0932-0113issn
1432-1955journal_volume
80pub_type
杂志文章abstract::Nematodes of the family Aspidoderidae (Nematoda: Heterakoidea) Freitas 1956 are widely distributed from Americas. The species of the genus Aspidodera Railliet and Henry 1912 are parasites of mammals of the orders Edentata, Marsupialia, and Rodentia. In the present work, Aspidodera raillieti (L. Travassos, Mem Inst Osw...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0641-5
更新日期:2007-10-01 00:00:00
abstract::This study describes the ultrastructural organisation of the spermatozoon of a digenean Enodiotrema reductum (Pligiorchiida: Plagiorchiidae) from the green turtle Chelonia mydas (Linnaeus, 1758). This is the first report of E. reductum from Senegal. The mature spermatozoon of E. reductum is filiform and exhibits two a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-2911-0
更新日期:2012-08-01 00:00:00
abstract::Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The librar...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050349
更新日期:1998-01-01 00:00:00
abstract::Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukar...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1742-0
更新日期:2010-04-01 00:00:00
abstract::Acute phase serum (APS) given at the time of challenge with Plasmodium berghei inhibited the generation to immunity to the infecting plasmodia. Administered with a single dose of vaccine, it inhibited induction of immunity by the vaccine. Three weekly doses, the last given two weeks before infection, induced immunity....
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00536022
更新日期:1987-01-01 00:00:00
abstract::We have identified the 2-Cys peroxiredoxin (Prx) from a bovine Babesia parasite, B. bovis. Prx is a recently described family of antioxidant enzymes that are highly conserved in eukaryotes and prokaryotes. B. bovis 2-Cys Prx (BbTPx-1) contained two conserved cysteine residues that corresponded to Cys47 and Cys170 of t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1587-6
更新日期:2009-10-01 00:00:00
abstract::Parasites from Flensburg fjord, northern German Baltic coast, were investigated under regard of the component, guild, and supra community level. At least, 28 host species of the guilds snails, planktonic and benthic crustaceans, and fish (exclusively small-sized species) were infected by 53 parasite species (28 autoge...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1218-7
更新日期:2009-01-01 00:00:00
abstract::Legionella were detected with the direct DNA extraction method, Legionella culture method, and free-living amoebae (FLA) culture method from weak alkaline carbonate spring water in Taiwan. Moreover, we also investigated the existence of Acanthamoeba, Hartmannella, and Naegleria, ubiquitous FLA in aquatic environments,...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2366-8
更新日期:2011-11-01 00:00:00
abstract::Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050419
更新日期:1998-05-01 00:00:00
abstract::Filter-feeding molluscan shellfish can concentrate environmentally derived waterborne pathogens of humans, which can be utilized in the sanitary assessment of water quality. In the present study, oocysts of Cryptosporidium were detected in Bent mussels (Ischadium recurvum) at two Chesapeake Bay sites from which C. par...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050590
更新日期:1999-07-01 00:00:00
abstract::Blastocystis hominis has been reclassified as a protozoan parasite. Its role as a human pathogen is somewhat controversial. There has been a dramatic increase in the frequency of B. hominis infection in association with diarrhea especially in immunocompromised hosts like AIDS patients, travelers, homosexuals, day care...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3652-4
更新日期:2014-01-01 00:00:00
abstract::Northern elephant seals (Mirounga angustirostris Gill, 1866), inhabiting rookeries on the mainland of Año Nuevo State Reserve in central California, were investigated in 2012 for presence of hookworms (Uncinaria spp.). Material collected and examined for hookworms included: blubber (n = 15), stomach and intestines (n ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-012-2919-5
更新日期:2012-10-01 00:00:00
abstract::This work investigated the anti-amoebic activity of two samarium (Sm) complexes, the acyclic complex [bis(picrato)(pentaethylene glycol)samarium(III)] picrate-referred to as [Sm(Pic)2(EO5)](Pic)-and the cyclic complex [bis(picrato)(18-crown-6)samarium(III)] picrate-referred to as [Sm(Pic)2(18C6)](Pic). Both Sm complex...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5814-x
更新日期:2018-05-01 00:00:00
abstract::The blowfly Lucilia sericata (Diptera: Calliphoridae) is a facultative ectoparasite that causes myiasis in both man and animals, leading to serious human health problems and economic losses in the livestock industry. Like other insects, olfaction of this species plays an important role in host location and is presumab...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2563-5
更新日期:2012-02-01 00:00:00
abstract::The fine structure of the infective hexacanths of Echinococcus multilocularis was examined with particular emphasis on the functional ultrastructure of penetration glands and nerve cells directly involved in the mechanism of initial host infection. The oncosphere contains two types of penetration glands, PG1 and PG2, ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5957-9
更新日期:2018-08-01 00:00:00
abstract::Common frogs (Rana temporaria) were exposed either to third-stage larvae (L3) or to first-stage larvae (L1) of Angiostrongylus vasorum. Following exposure to L3, viable larvae could be detected in the frogs for at least 2 weeks. Following exposure to L1, the frogs developed viable L3 in their tissues within 30 days. I...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931834
更新日期:1993-01-01 00:00:00
abstract::Disseminated visceral coccidiosis (DVC) is a widely distributed intestinal and extraintestinal disease of cranes caused by eimeriid coccidia and has lethal pathogenicity to several crane species. Here, feces of 164 black-necked cranes collected in Dashanbao Black-necked Crane National Nature Reserve, China, were exami...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5765-2
更新日期:2018-03-01 00:00:00
abstract::Praziquantel is still very effective for the treatment of schistosomiasis, but there are rising concerns on the potential risk of developing resistances because of the extensive use of this drug. Triclabendazole, a systemic anthelmintic, is very effective against other trematodes such as Paragonimus spp. and Fasciola ...
journal_title:Parasitology research
pub_type: 临床试验,杂志文章
doi:10.1007/s00436-007-0802-6
更新日期:2008-03-01 00:00:00
abstract::In opposite to the free-living soil nematode Caenorhabditis elegans, the genetic regulation of hypobiosis or inhibited or arrested development in parasitic nematodes is completely unknown. In C. elegans, the daf-genes or the age-1 gene are of major importance in signaling pathways regulating arrested development. To i...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0594-8
更新日期:2007-09-01 00:00:00
abstract::An experiment was conducted to study the adjuvant effect of ginsomes on the recombinant profilin in coccidian-infected breeding birds. Three-day-old chickens were vaccinated with Eimeria tenella recombinant profilin antigen (10, 50, and 100 μg per chicken) with or without 50 μg ginsomes per chicken. The boost vaccinat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-011-2784-7
更新日期:2012-06-01 00:00:00
abstract::Two thousand four hundred fifty-eight fish comprised of 53 species were captured in the Danjiangkou Reservoir, in the northwestern part of Hubei Province, central China during 2004, to examine Camallanus cotti infections. We found that 19 cypriniform, 3 siluriforme, and 4 perciforme fishes were infected by the nematod...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0472-4
更新日期:2007-07-01 00:00:00
abstract::In the gastrointestinal mucosa, mucus produced by goblet cells plays an important role in the defense against various pathogens. It is well known that some helminth parasites are able to up-regulate goblet cell numbers and alter the mucus components. However, the nature of the interactions between the protozoan parasi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1256-1
更新日期:2009-03-01 00:00:00
abstract::Isometamidium chloride (ISM) is an effective trypanocide with curative and prophylactive activity in husbandry animals. The mode of action of ISM against pathogenic trypanosomes is not fully understood, but there is evidence in the literature that kinetoplastic topoisomerase type II is selectively inhibited by the dru...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050346
更新日期:1997-01-01 00:00:00
abstract::Naturally occurring bithyniid snails, Bithynia siamensis goniomphalos (Prosobranchia: Bithyniidae), and their intermediate hosts were sampled from Khammouane Province, the Lao People's Democratic Republic, and the prevalence of the carcinogenic human liver fluke, Opisthorchis viverrini, was examined. The presence of O...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2140-3
更新日期:2011-04-01 00:00:00
abstract::Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s004360050568
更新日期:1999-05-01 00:00:00
abstract::During a survey lasting from 1990 to 2008, we captured 4,113 Asian house rats, Rattus tanezumi Temminck 1844 (Rodentia: Muridae) from 28 counties of Yunnan Province in southwestern China. From these rats, a total of 19,304 gamasid mites (Acari: Mesostigmata) were collected and identified as comprising 50 different spe...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-013-3354-y
更新日期:2013-05-01 00:00:00
abstract::The migration of Schistosoma japonicum and S. mansoni through mouse skin epidermis and dermis was compared by immunofluorescence techniques from 4 to 22 h after infection. At all times, the percentage of parasites detected in the dermis was significantly higher for S. japonicum than for S. mansoni. Thus, S. japonicum ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-004-1284-4
更新日期:2005-02-01 00:00:00
abstract::Sarcocystis sarcocysts are common in many species of domestic and wild animals. Here, we report sarcocystosis in muscles from 91 free range elk (Cervus elaphus) from Pennsylvania, USA, tested by histopathology, transmission electron microscopy (TEM), and DNA sequencing. Sarcocysts were detected in hematoxylin and eosi...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6024-2
更新日期:2018-10-01 00:00:00
abstract::Intraspecific variation in Schistosoma mansoni infection and modulation of its expression by vertebrate host genetics was studied by evaluation of some biological parameters of the infection in BALB/c and C57BL/6 mice infected with one Brazilian (BH) and two Venezuelan (YT and SM) laboratory strains of the parasite. M...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/pl00008565
更新日期:2001-02-01 00:00:00
abstract::The catalysis of malate dehydrogenase (MDH) in Plasmodium falciparum (pfMDH) which involves NAD/NADH coupling is crucial for the parasite's pathogenicity. Primers were designed based on the P. falciparum genome resource, and these facilitated the cloning of a gene coding for pfMDH from a local clinical isolate. The DN...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-0996-1
更新日期:2004-01-01 00:00:00