Abstract：
:Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The library contains a tenfold E. tenella genome equivalent consisting of 3177 clones arrayed in 96-well microtitre plates and gridded on nylon membranes at 1728 clones/86 cm2. Size fractionation of a random sample of 185 YACs revealed an average insert size of approximately 170 kb. Hybridisation of four E. tenella single-copy probes to the gridded colony filters produced around four or more positive clones. A probe representing the whole of the 1.2-Mb chromosome 2, excised from a preparative gel following pulsed-field gel electrophoresis, revealed between 80 and 100 positive clones.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Hoogendoorn B,Toye AA,Bumstead N,Shirley MWdoi
10.1007/s004360050349subject
Has Abstractpub_date
1998-01-01 00:00:00pages
13-6issue
1eissn
0932-0113issn
1432-1955journal_volume
84pub_type
杂志文章abstract：:Malaria is caused by unicellular parasites of the genus Plasmodium, which reside in erythrocytes during the clinically relevant stage of infection. To separate parasite from host cell material, haemolytic agents such as saponin are widely used. Previous electron microscopy studies on saponin-treated parasites reported...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06932-9
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journal_title：Parasitology research
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pub_type： 杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章,评审
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doi：10.1007/BF00931020
更新日期：1991-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-01-01 00:00:00
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更新日期：2015-06-01 00:00:00
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