Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis.


:Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukaryotic expression vector pVAX I, and the pVAX-MIC8 expression plasmid was constructed, and the plasmid diluted with PBS to 100 mg/100 microl was injected into the Kunming mice muscularly. Levels of IgG antibody, gamma-interferon (IFN-gamma), interleukin-2 (IL-2), interleukin-4, and interleukin-10 were detected. The mice were challenged with tachyzoites of the virulent T. gondii RH strain at the 14th day after the last immunization to observe the survival time. The high level of IFN-gamma, IL-2, and IgG antibody indicated that mice vaccinated with recombinant pVAX-MIC8 plasmid could elicit strong cellular and humoral immune responses and showed a significantly increased survival time (10.3 +/- 0.9 days) compared with control mice which died within 5 days of challenge infection. These data demonstrate that the T. gondii MIC8 is a potential vaccine candidate against toxoplasmosis.


Parasitol Res


Parasitology research


Liu MM,Yuan ZG,Peng GH,Zhou DH,He XH,Yan C,Yin CC,He Y,Lin RQ,Song HQ,Zhu XQ




Has Abstract


2010-04-01 00:00:00












  • Immune responses of the domestic fowl to Dermanyssus gallinae under laboratory conditions.

    abstract::There appear to be few reports in the literature regarding the host-poultry red mite (Dermanyssus gallinae) immunological relationship, despite the negative impact D. gallinae can have on both bird welfare and egg production, as well as its potential to act as a reservoir of zoonotic infections. The current study inve...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Harrington DW,Robinson K,Sparagano OA

    更新日期:2010-05-01 00:00:00

  • Molecular karyotypes of Eimeria tenella resolved by PFGE: an evaluation of different agaroses.

    abstract::Molecular karyotypes that revealed size polymorphisms between homologous forms of chromosomes 1, 2, 3 or 4 in three strains of the coccidial parasite Eimeria tenella were derived by pulsed field gel electrophoresis (PFGE) using brands of agaroses marketed either specifically for PFGE or for purposes such as protein el...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Sheriff R,Carroll F,Shirley MW

    更新日期:2003-03-01 00:00:00

  • Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae).

    abstract::Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with st...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Montresor LC,Vidigal TH,Mendonça CL,Fernandes AA,de Souza KN,Carvalho OS,Caputo LF,Mota EM,Lenzi HL

    更新日期:2008-11-01 00:00:00

  • Sarcocystis oreamni, n. sp. (Apicomplexa: Sarcocystidae) from the mountain goat (Oreamnos americanus).

    abstract::Numerous species of Sarcocystis have been reported from wild ruminants, but none has been named from the Rocky Mountain goat (Oreamnos americanus). Mature sarcocysts were found in frozen muscle samples of three of seven mountain goats from Alaska, USA. Two morphological types of sarcocysts were found; one had Sarcocys...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Calero-Bernal R,Van Wilpe E,White K,Verma SK,Cerqueira-Cézar CK,Dubey JP

    更新日期:2015-11-01 00:00:00

  • Relationships between vector-borne parasites and free-living mammals at the Brazilian Pantanal.

    abstract::In nature, parasitic infections must be addressed as complex systems involving parasite-host relationships on a temporal and spatial scale. Since the parasites cover a great biological diversity, we can expect that wildlife are exposed simultaneously to different parasites. In this sense, the objective of this work wa...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Santos FM,de Sousa KCM,Sano NY,Nantes WAG,Liberal SC,Machado RZ,André MR,Herrera HM

    更新日期:2021-01-09 00:00:00

  • Status of tick distribution in Bangladesh, India and Pakistan.

    abstract::On a global basis, ticks transmit a greater variety of pathogenic microorganisms, protozoa, rickettsiae, spirochaets, and viruses than any other arthropods and are among the most important vectors of diseases affecting livestock, humans, and companion animals. Ticks and tick-borne diseases (TTBDs) affect 80% of the wo...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Ghosh S,Bansal GC,Gupta SC,Ray D,Khan MQ,Irshad H,Shahiduzzaman M,Seitzer U,Ahmed JS

    更新日期:2007-09-01 00:00:00

  • Monitoring of alien mosquitoes of the genus Aedes (Diptera: Culicidae) in Austria.

    abstract::Systematic, continuous mosquito surveillance is considered the most reliable tool to predict the spread and establishment of alien mosquito species such as the Asian tiger mosquito (Aedes albopictus), Japanese bush mosquito (Aedes japonicus), and the transmission risk of mosquito-borne arboviruses to humans. Only sing...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Schoener E,Zittra C,Weiss S,Walder G,Barogh BS,Weiler S,Fuehrer HP

    更新日期:2019-05-01 00:00:00

  • Proteolytic activity of the Gymnorhynchus gigas plerocercoid: purification and properties of a collagenase from the crude extract.

    abstract::The present report demonstrates that the Gymnorhynchus gigas plerocercoid possesses various types of endo- and exoproteases with activity against general (azocoll, azocasein, and azoalbumin) and specific substrates (elastin, keratin, collagen, hemoglobin, fibrinogen, plasma, and immunoglobulin G). The activity against...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Vázquez-López C,de Armas-Serra C,Giménez-Pardo C,Rodríguez-Caabeiro F

    更新日期:1999-01-01 00:00:00

  • Fasciola hepatica: the effect of the sodium ionophore monensin on the adult tegument.

    abstract::The effect on the tegument of adult Fasciola hepatica of incubation in the sodium ionophore monensin, the Na+/K+-ATPase inhibitor ouabain and ouabain pretreatment followed by monensin has been determined in vitro by scanning and transmission electron microscopy (SEM, TEM). With monensin incubation alone (1 x 10(-6) M)...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Skuce PJ,Fairweather I

    更新日期:1989-01-01 00:00:00

  • Inhibitory effects of novel ciprofloxacin derivatives on the growth of four Babesia species and Theileria equi.

    abstract::The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Batiha GE,Tayebwa DS,Beshbishy AM,N'Da DD,Yokoyama N,Igarashi I

    更新日期:2020-09-01 00:00:00

  • Immunopathological assessments of human Blastocystis spp. in experimentally infected immunocompetent and immunosuppresed mice.

    abstract::Blastocystis spp., one of the most common parasites colonizing the human intestine, is an extracellular, luminal protozoan with controversial pathogenesis. The host's immune response against Blastocystis spp. infection has also not been defined yet. Therefore, this research aimed to assess the potential pathogenicity ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Abdel-Hafeez EH,Ahmad AK,Abdelgelil NH,Abdellatif MZ,Kamal AM,Hassanin KM,Abdel-Razik AR,Abdel-Raheem EM

    更新日期:2016-05-01 00:00:00

  • Lethal efficacy of extract from Zingiber officinale (traditional Chinese medicine) or [6]-shogaol and [6]-gingerol in Anisakis larvae in vitro.

    abstract::The authors previously reported that an extract from Zingiber officinale, traditionally eaten along with raw fish and used in traditional Chinese medicine, effectively destroyed Anisakis larvae in vitro. In this study, we analyzed the effective components of ginger rhizomes. Methanol extracts were fractionated after f...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Goto C,Kasuya S,Koga K,Ohtomo H,Kagei N

    更新日期:1990-01-01 00:00:00

  • Anaphylactic shock ensuing therapeutic puncture of an echinococcal cyst.

    abstract::Cystic echinococcosis (CE) is a widespread zoonosis. For treating single echinococcal cysts during the last decades, therapeutic puncture of the cyst, aspiration, injection of a scolicide, and re-aspiration (PAIR) has been established as a minimal-invasive alternative method to surgery. A recent review on the complica...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Richter J,Profis E,Holtfreter MC,Orhun A,Müller-Stöver I,Dedelen H,Kubitz R

    更新日期:2015-02-01 00:00:00

  • Efficacy of mangiferin against Cryptosporidium parvum in a neonatal mouse model.

    abstract::The inhibitory activity of mangiferin (50 mg/kg/die and 100 mg/kg/die) on Cryptosporidium parvum was evaluated in a neonatal mouse model and its activity was compared with that of paromomycin (100 mg/kg/die). At 4 days of age, neonatal Swiss conventional outbred mice were experimentally infected by oral administration...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Perrucci S,Fichi G,Buggiani C,Rossi G,Flamini G

    更新日期:2006-07-01 00:00:00

  • Characterization and bioassay for larvicidal activity of Anacardium occidentale (cashew) shell waste fractions against dengue vector Aedes aegypti.

    abstract::Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of A...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Torres RC,Garbo AG,Walde RZ

    更新日期:2015-10-01 00:00:00

  • Detection of Blastocystis hominis: a controversial human pathogen.

    abstract::Blastocystis hominis has been reclassified as a protozoan parasite. Its role as a human pathogen is somewhat controversial. There has been a dramatic increase in the frequency of B. hominis infection in association with diarrhea especially in immunocompromised hosts like AIDS patients, travelers, homosexuals, day care...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Basak S,Rajurkar MN,Mallick SK

    更新日期:2014-01-01 00:00:00

  • A fast and inexpensive method for isolation of high-quality RNA from eimerian oocysts.

    abstract::An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h. ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Johnston DA,Bratty J,Fernando MA

    更新日期:1998-07-01 00:00:00

  • Prevalence and distribution of Cryptosporidium spp. in dairy cattle in Heilongjiang Province, China.

    abstract::Few data are available on the molecular characterization of Cryptosporidium spp. in cattle in China. In the present study, a total of 507 fecal specimens from six dairy farms in Heilongjiang Province were examined for Cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentatio...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Liu A,Wang R,Li Y,Zhang L,Shu J,Zhang W,Feng Y,Xiao L,Ling H

    更新日期:2009-09-01 00:00:00

  • Role of CCR5Δ32 mutation in protecting patients with Schistosoma mansoni infection against hepatitis C viral infection or progression.

    abstract::Schistosomiasis has been incriminated in the significant increase in hepatitis C virus (HCV) infections, although the association has not been adequately explained. We hypothesized that the CCR5Δ32 mutation may be involved in the high prevalence of HCV with schistosomiasis. The aim was to explore the association betwe...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: El-Moamly AA,El-Sweify MA,Rashad RM,Abdalla EM,Ragheb MM,Awad MM

    更新日期:2013-07-01 00:00:00

  • Larvicidal activity of micronized aqueous suspension of calcium hydroxide against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae).

    abstract::In the search of alternatives for the control of mosquitoes of medical importance, we evaluated the larvicidal activity of micronized suspensions of calcium hydroxide [Ca(OH)₂] against Aedes aegypti and Culex quinquefasciatus. Tests conducted under laboratory conditions determined a LC50 = 0.027% (LC90 = 0.096%) for A...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Estrada-Aguilar A,Sánchez-Manzano RM,Martínez-Ibarra JA,Camacho AD,Márquez-Navarro A,Nogueda-Torres B

    更新日期:2012-03-01 00:00:00

  • Clinical implications of a gradual dormancy concept in malaria.

    abstract::Malaria recurrences after an initially successful therapy and malarial fever occurring a long time after infection are well-known problems in malariology. Currently, two distinct types of malaria recurrences are defined: recrudescence and relapse. A recrudescence is thought to originate from circulating Plasmodium blo...

    journal_title:Parasitology research

    pub_type: 杂志文章,评审


    authors: Richter J,Franken G,Holtfreter MC,Walter S,Labisch A,Mehlhorn H

    更新日期:2016-06-01 00:00:00

  • Ultrastructural observations of the attachment organs of the monogenean Rajonchocotyle emarginata (Olsson, 1876) (Polyopisthocotylea: Hexabothriidae), a gill parasite of rays.

    abstract::The present study uses scanning and transmission electron microscopical observations to examine the haptoral features of the hexabothriid polyopisthocotylean monogenean Rajonchocotyle emarginata from the gills of the elasmobranch Amblyraja radiata. The haptor possesses six equal, pedunculate suckers, each armed with a...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Poddubnaya LG,Hemmingsen W,Gibson DI

    更新日期:2016-06-01 00:00:00

  • Biological activity of Paecilomyces genus against Toxocara canis eggs.

    abstract::Saprophytic soil fungi can exert ovicidal and ovistatic effects on helminths with differing degrees of efficiency. The representatives of such fungi from temperate regions, Paecilomyces lilacinus (Thom) Samson and P. marquandii (Masse) Hughes, exhibit recognized ovicidal activity on some nematodes. We evaluated the ac...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Basualdo JA,Ciarmela ML,Sarmiento PL,Minvielle MC

    更新日期:2000-10-01 00:00:00

  • The first detection of Cryptosporidium deer-like genotype in cattle in Japan.

    abstract::The general perception is that cattle are major reservoirs for Cryptosporidium parvum infections in humans and that C. parvum is a major cause of diarrhea and production loss in cattle. Adult cattle may play an important role as cryptic carrier of the infection. Cryptosporidium spp. in asymptomatic adult dairy cattle ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Amer S,Honma H,Ikarashi M,Oishi R,Endo M,Otawa K,Nakai Y

    更新日期:2009-03-01 00:00:00

  • Ticks and tick-borne pathogens in wild birds in Greece.

    abstract::Wild birds are common hosts of ticks and can transport them for long distances, contributing to the spreading of tick-borne pathogens. The information about ticks on birds and tick-borne pathogens in Greece is limited. The present study aimed to evaluate the prevalence and species of ticks infesting wild resident bird...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Diakou A,Norte AC,Lopes de Carvalho I,Núncio S,Nováková M,Kautman M,Alivizatos H,Kazantzidis S,Sychra O,Literák I

    更新日期:2016-05-01 00:00:00

  • Alien parasites on an alien fish species: monogeneans from the black bullhead Ameiurus melas (Siluriformes) in the Lake Srebarna Biosphere Reserve, Bulgaria, with the first record of Gyrodactylus nebulosus in the Palaearctic.

    abstract::Black bullhead Ameiurus melas (Actinopterygii: Siluriformes) is an alien fish species of North American origin, which has expanded its invasive geographical range in Europe. In 2017-2019, 32 black bullhead specimens from the Lake Srebarna Biosphere Reserve, Bulgaria, were examined for monogenean parasites. Two species...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Vancheva N,Bobeva A,Pehlivanov L,Stefanov T,Georgiev BB

    更新日期:2020-07-01 00:00:00

  • Consequences of eye fluke infection on anti-predator behaviours in invasive round gobies in Kalmar Sound.

    abstract::Larvae of the eye fluke, Diplostomum, emerge from snails and infect fish by penetrating skin or gills, then move to the lens where they may impair the vision of the fish. For the fluke to reproduce, a bird must eat the infected fish, and it has been suggested that they therefore actively manipulate the fish's behaviou...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Flink H,Behrens JW,Svensson PA

    更新日期:2017-06-01 00:00:00

  • Towards the Toxoplasma gondii proteome: position of 13 parasite excretory antigens on a standardized map of two-dimensionally separated tachyzoite proteins.

    abstract::High resolution two-dimensional separation of Toxoplasma gondii tachyzoite lysate revealed up to 224 distinct protein spots in Coomassie-stained gel. Computional matching of 14 digitized gels yielded a standard two-dimensional proteome map. The excretory T. gondii dense granule proteins GRA1-GRA8, S16/acid phosphatase...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Dlugonska H,Dytnerska K,Reichmann G,Stachelhaus S,Fischer HG

    更新日期:2001-08-01 00:00:00

  • Cloning and characterization of a novel enzyme: tyrosine hydroxylase from Schistosoma japonicum.

    abstract::Catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including Schistosoma japonicum. S. japonicum tyrosine hydroxylase (SjTH) was amplified by rapid amplification of cDNA ends polymerase chain reaction that shows strong homo...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Hu Y,Shi D,Luo Q,Liu Q,Zhou Y,Liu L,Yu L,Wei W,Shen J

    更新日期:2011-10-01 00:00:00

  • Development of a highly sensitive IgG-ELISA based on recombinant arginine kinase of Toxocara canis for serodiagnosis of visceral larva migrans in the murine model.

    abstract::Toxocariasis is a worldwide zoonotic disease caused by the ascarid nematode Toxocara canis. The most common method available for serodiagnosis of toxocariasis is an enzyme-linked immunosorbent assay (ELISA) test using Toxocara excretory-secretory antigen (TES). The present study describes the development of IgG-ELISA ...

    journal_title:Parasitology research

    pub_type: 杂志文章


    authors: Wickramasinghe S,Yatawara L,Nagataki M,Takamoto M,Watanabe Y,Rajapakse RP,Uda K,Suzuki T,Agatsuma T

    更新日期:2008-09-01 00:00:00