Abstract：
:Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukaryotic expression vector pVAX I, and the pVAX-MIC8 expression plasmid was constructed, and the plasmid diluted with PBS to 100 mg/100 microl was injected into the Kunming mice muscularly. Levels of IgG antibody, gamma-interferon (IFN-gamma), interleukin-2 (IL-2), interleukin-4, and interleukin-10 were detected. The mice were challenged with tachyzoites of the virulent T. gondii RH strain at the 14th day after the last immunization to observe the survival time. The high level of IFN-gamma, IL-2, and IgG antibody indicated that mice vaccinated with recombinant pVAX-MIC8 plasmid could elicit strong cellular and humoral immune responses and showed a significantly increased survival time (10.3 +/- 0.9 days) compared with control mice which died within 5 days of challenge infection. These data demonstrate that the T. gondii MIC8 is a potential vaccine candidate against toxoplasmosis.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Liu MM,Yuan ZG,Peng GH,Zhou DH,He XH,Yan C,Yin CC,He Y,Lin RQ,Song HQ,Zhu XQdoi
10.1007/s00436-010-1742-0subject
Has Abstractpub_date
2010-04-01 00:00:00pages
1079-84issue
5eissn
0932-0113issn
1432-1955journal_volume
106pub_type
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