Abstract：
:This report describes a technique for studying the adherence and growth of Giardia intestinalis trophozoites (strains PARIS/86/LCF/1, PARIS/86/LCF/2 and PARIS/88/LCF/8) using the human colon carcinoma cell line Caco2. Giardia trophozoites were cultured with Caco2 cells in a modified HSP3 culture medium. The biochemical differentiation of Caco2 cells was established by an increase in sucrase isomaltase activities to values of 4.51 +/- 0.90 and 10.39 +/- 3.00 milliunits/mg protein for 8- and 12-day-old cultures, respectively. Giardia, adherence to 8- and 12-day-old Caco2 cells reached a value of greater than 75% after 60 and 30 min, respectively. Adherence diminished significantly at 24 degrees C and was almost undetectable at 4 degrees C. Depletion of divalent cations reduced the proportion of adherent trophozoites by up to 46%. Adherence was pH-independent between pH 6.0 and 7.6. Parasite growth increased when Caco2 cell monolayers were used instead of axenic cultures. This in vitro human cell model may contribute to the study of the mechanisms and factors involved in the host-parasite interaction.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Favennec L,Chochillon C,Meillet D,Magne D,Savel J,Raichvarg D,Gobert JGdoi
10.1007/BF00932566subject
Has Abstractpub_date
1990-01-01 00:00:00pages
581-4issue
7eissn
0932-0113issn
1432-1955journal_volume
76pub_type
杂志文章abstract：:In freshwater ecosystems, snails can significantly influence the competition between primary producers through grazing of periphyton. This activity can potentially be modified by trematodes, a large group of parasites which mostly use molluscs as the first intermediate host. Available studies, however, show contradict...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6052-y
更新日期：2018-11-01 00:00:00
abstract：:Availability of mites is a recognized limiting factor of biological and genetic investigations of the genus Sarcoptes. Current methods of deoxyribonucleic acid (DNA) extraction from individual mites also need substantial improvement in efficiency and operator friendliness. We have first developed a technique for effic...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1580-0
更新日期：2009-10-01 00:00:00
abstract：:Prevalence and intensity of gastrointestinal parasites were studied through a longitudinal survey in 400 horses over a 17-month period in an abattoir in Germany. Three hundred and ten horses (77.5 %) were demonstrated harbouring endoparasites either by direct recovery of parasites from the digestive tract and/or in te...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3150-0
更新日期：2013-01-01 00:00:00
abstract：:The efficacy of a combination of ivermectin (IVM) and albendazole (ABZ) used as a chemoprophylactic treatment and as treatment of secondary hydatidosis is described for the first time. IVM treatment alone was not effective against Echinococcus granulosus, either when the protoscolices were recently inoculated or when ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0623-6
更新日期：2002-06-01 00:00:00
abstract：:Immunoscreening of an adult Schistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and i...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931812
更新日期：1989-01-01 00:00:00
abstract：:The narrow Andean-Patagonian temperate rainforest strip in the west of southern South America is inhabited by two endemic species of cervids, the southern pudu (Pudu puda) and the huemul (Hippocamelus bisulcus), both cataloged as near threatened and threatened species, respectively. One of the possible causes of their...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5429-7
更新日期：2017-05-01 00:00:00
abstract：:Discoid granules were isolated from the tegument of adult Schistosoma mansoni by differential centrifugation of freeze-thaw extracts. Comparison of proteins and glycoproteins from discoid granules, surface membranes and the soluble fraction implied that discoid granules and the surface membrane were functionally linke...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00539573
更新日期：1988-01-01 00:00:00
abstract：:Experimental infections of Galba truncatula with Fasciola gigantica or F. hepatica were carried out under laboratory conditions (20 degrees C) to determine the characteristics of rediae of both species via their morphometry and to find reliable measurements that might be efficiently used to discriminate between the re...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-003-0966-7
更新日期：2003-11-01 00:00:00
abstract：:Introduced Siberian chipmunks Eutamias sibiricus have been reported to be important reservoirs for human Lyme disease, as they may host high numbers of hard ticks carrying Borrelia spp. and other pathogens. In the present study, we assessed the prevalence of Borrelia spp. and other pathogenic bacteria in ectoparasite ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6022-4
更新日期：2018-10-01 00:00:00
abstract：:A key challenge in the successful treatment of Acanthamoeba infections is its ability to transform into a dormant cyst form that is resistant to physiological conditions and pharmacological therapies, resulting in recurrent infections. The carbohydrate linkage analysis of cyst walls of Acanthamoeba castellanii showed ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3254-6
更新日期：2013-03-01 00:00:00
abstract：:Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of plant origin my serve as an alternative biocontrol technique in the future. The aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extra...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4856-6
更新日期：2016-03-01 00:00:00
abstract：:Snake venom can affect the growth of Trypanosoma cruzi and Leishmania spp. As new classes of therapeutic drugs against protozoan parasites could be derived from snake venom, alterations in the ultrastructure and growth of the epimastigotes, trypomastigotes and amastigotes of T. cruzi, as well as the promastigotes of L...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0626-3
更新日期：2002-07-01 00:00:00
abstract：:Pyriproxyfen, a juvenile hormone analogue, diflubenzuron, a chitin synthesis inhibitor, and azadirachtin, an ecdysone agonist, are three insect growth regulators (IGRs) considered as selective and effective insecticides for mosquitoes. Romanomermis iyengari (Welch) is a mosquito-parasitic mermithid that can provide bi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-3201-6
更新日期：2013-02-01 00:00:00
abstract：:Ticks spend most of their life cycle in the environment, and all tick life cycle stages are dependent on a complex combination of climate variables. Furthermore, host availability and vegetation significantly modulate the dynamics of tick populations. Tick recruitment is dependent on successful reproduction, which in ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-008-1056-7
更新日期：2008-12-01 00:00:00
abstract：:Several rhoptry proteins (ROPs) have been confirmed to be critical virulence factors of Toxoplasma gondii strains from North America and Europe. The two active kinases ROP17 and ROP18, and pseudokinase ROP5 were thought to be the key determinants of parasites' virulence in laboratory mice. Given the genetic diversity ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5141-z
更新日期：2016-10-01 00:00:00
abstract：:Atractolytocestus tenuicollis (Li, 1964) Xi, Wang, Wu, Gao et Nie, 2009 is a monozoic, non-segmented tapeworm of the order Caryophyllidea, parasitizing exclusively common carp (Cyprinus carpio L.). In the current work, the first molecular data, in particular complete ribosomal internal transcribed spacer 2 (ITS2) and ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3516-y
更新日期：2013-10-01 00:00:00
abstract：:Direct wet mount examination and concentration are the most commonly used methods for detecting intestinal parasites from fecal samples. Concentration methods are used when there are fewer protozoan cyst, coccidian oocyst, microsporidial spore, helminth egg, and larvae in the fecal samples. Early detection of the caus...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3899-4
更新日期：2014-07-01 00:00:00
abstract：:Two placebo-controlled pen studies were conducted to assess the efficacy of an imidacloprid 10 %/flumethrin 4.5 % polymer matrix collar (Seresto®, Bayer; Investigational Veterinary Product (IVP)) against the Australian paralysis tick (Ixodes holocyclus). Dogs assigned to the placebo (n = 8) or IVP (n ≥ 8) groups had c...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-013-3276-8
更新日期：2013-08-01 00:00:00
abstract：:Feline lungworms infect the respiratory tract of wild and domestic cats, causing infection often associated with clinical disease. Until recently, Aelurostrongylus abstrusus has been considered the most relevant species of lungworm, while Troglostrongylus brevior was considered of less significance. Fecal samples of f...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06852-8
更新日期：2020-10-01 00:00:00
abstract：:The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0541-8
更新日期：2007-08-01 00:00:00
abstract：:This randomised controlled laboratory study demonstrated the residual speed of efficacy of an imidacloprid/flumethrin collar (Seresto(®), Bayer) for the control of ticks (Dermacentor variabilis, Amblyomma americanum) at 6 and 12 hours postinfestation on dogs when compared to oral afoxolaner (NexGard(®), Merial). Dogs ...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-015-4515-y
更新日期：2015-08-01 00:00:00
abstract：:Understanding the fundamental factors influencing the epidemiology of wildlife disease is essential to determining the impact of disease on individual health and population dynamics. The host-pathogen-environment relationship of the endangered Australian sea lion (Neophoca cinerea) and the parasitic hookworm, Uncinari...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3997-3
更新日期：2014-09-01 00:00:00
abstract：:Spirocerca vulpis is a parasitic nematode of red foxes associated with gastric nodule formation. Since its description in 2018, this species has been reported in red foxes from Spain, Bosnia and Herzegovina, Italy, and Portugal. We present here the analysis of uncharacterized nematodes obtained from gastric nodules of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06791-4
更新日期：2020-09-01 00:00:00
abstract：:Limited data is available on the vertical transmission of Neospora caninum via the colostrum. The results of our previous research revealed the presence of N. caninum DNA in the milk of seropositive cows. The aim of the present work is to demonstrate parasite DNA in colostrum samples. A polymerase chain reaction using...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0288-7
更新日期：2007-02-01 00:00:00
abstract：:Ruminant coccidiosis is a gastrointestinal disease caused by parasites of the genus Eimeria. Environmental and climatic factors are relevant for the development, survival, and transmission of coccidiosis because Eimeria oocysts are able to survive in the environment for several weeks or months in favorable conditions ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06548-8
更新日期：2020-03-01 00:00:00
abstract：:Toxoplasma gondii is an important zoonotic protozoan of the phylum Apicomplexa that can infect nearly all warm-blooded animals. The parasite can exist as the interconvertible tachyzoite or bradyzoite forms, leading to acute or latent infection, respectively. No drug has been reported to penetrate the cyst wall and red...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06722-3
更新日期：2020-07-01 00:00:00
abstract：:Cysticercoids as well as 6-, 10-, and 14-day Hymenolepis diminuta were evaluated in terms of enzymatic activities related to phosphoenolpyruvate (PEP) utilization and mitochondrial succinate accumulation. The data obtained support a transition toward anaerobic electron-transport-dependent succinate accumulation, chara...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050487
更新日期：1998-10-01 00:00:00
abstract：:We examine the taxonomic composition and endemism of adult helminth parasites of freshwater fishes of Mexico, with regard to the main hydrological basins of the country. A presence-absence matrix, including every species of adult helminth parasites of freshwater fishes from 23 Mexican hydrological basins was compiled ...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-012-3175-4
更新日期：2013-01-01 00:00:00
abstract：:Malarial antigens on the surface of infected erythrocytes have been described by many investigators. However, few of these antigens have been unambiguously demonstrated to be exposed on the surface of erythrocytes. This study demonstrates that mild glutaraldehyde fixation results in the cytoplasmic face of the host me...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932242
更新日期：1993-01-01 00:00:00
abstract：:The purpose of our study was to assess the effects of experimental dicroceliosis on the antioxidant defense capability of the liver in hamsters. Studies were carried out at 80 and 120 days after infection with an oral dose of 40 metacercariae of Dicrocoelium dendriticum. The parasitic pathology was ascertained by the ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050579
更新日期：1999-06-01 00:00:00
