Abstract：
:Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABC1, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing six potential membrane-spanning segments. A single discontinuous ATP-binding element was located in the C-terminal region of TpABC1. The second gene, TpABC2, also contains a single C-terminal ATP-binding motif. Copies of TpABC2 were present at four loci in the T. parva genome on three different chromosomes. TpABC1 exhibited allelic polymorphism between stocks of the parasite. Comparison of cDNA and genomic sequences revealed that TpABC1 contained seven short introns, between 29 and 84 bp in length. The full-length TpABC1 protein was expressed in insect cells using the baculovirus system. Application of antibodies raised against the recombinant antigen to western blots of T. parva piroplasm lysates detected an 85 kDa protein in this life-cycle stage.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Kibe MK,Macklin M,Gobright E,Bishop R,Urakawa T,ole-MoiYoi OKdoi
10.1007/s004360100433subject
Has Abstractpub_date
2001-09-01 00:00:00pages
741-50issue
9eissn
0932-0113issn
1432-1955journal_volume
87pub_type
杂志文章abstract：:In July 2005, 107 rainbow trout in age 1+ from a salmonid farm in Southern Germany situated in the southern tributary area of the Danube river were examined. The aim of this study was to determine the gyrodactylid species found on rainbow trout and to identify their location on the host's body. In total, 291 specimens...
journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
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更新日期：2012-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2005-10-01 00:00:00
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更新日期：2015-12-01 00:00:00
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journal_title：Parasitology research
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更新日期：2011-05-01 00:00:00
abstract：:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Białowieża Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracel...
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