Molecular characterization of Hepatozoon canis from farm dogs in Pakistan.

Abstract:

:Hepatozoon canis is a tick-borne pathogen of canids, which is distributed worldwide. However, very little is known about this protozoan parasite in Pakistan. This study provides the first molecular evidence of H. canis from farm dogs from three agro-ecological zones of Punjab, Pakistan. A conventional PCR targeting the 18S rRNA gene was used to characterize H. canis from farm dogs from three districts, namely Kasur, Rawalpindi, and Muzaffargarh, in Punjab. Of 341 blood samples tested, 155 (45.5%) were positive for H. canis, 73 (61.3%) from Kasur, 46 (42.5%) from Rawalpindi, and 36 (31.5%) from Muzaffargarh. Phylogenetic analyses revealed that 18S rRNA sequences of H. canis from this study clustered in three clades with those of H. canis from previously published studies to the exclusion of all other Hepatozoon spp. included in the analysis. This study provides the first insight into H. canis from farm dogs in Pakistan. Furthermore, it lays a foundation for future studies of the parasite to assess the impact of canine hepatozoonosis in dogs from various agro-ecological zones in Pakistan where pet ownership of dogs is increasing.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Ahmad AS,Saeed MA,Rashid I,Ashraf K,Shehzad W,Traub RJ,Baneth G,Jabbar A

doi

10.1007/s00436-018-5790-1

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

1131-1138

issue

4

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-018-5790-1

journal_volume

117

pub_type

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