An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles.

Abstract:

:An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Vanholme B,De Meutter J,Tytgat T,Gheysen GD,Vanhoutte I,Gheysen GD

doi

10.1007/s00436-002-0661-0

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

731-3

issue

8

eissn

0932-0113

issn

1432-1955

journal_volume

88

pub_type

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