Abstract：
:Differentiation between viable and non-viable hookworm ova in environmental samples is necessary in order to implement strategies to mitigate re-infections in endemic regions. In this study, an untargeted metabolic profiling method was developed that utilised gas chromatography-mass spectrometry (GC-MS) in order to investigate hookworm ova viability. Ancylostoma caninum was used to investigate the metabolites within viable and non-viable ova. Univariate and multivariate statistical analyses of the data resulted in the identification of 53 significant metabolites across all hookworm ova samples. The major compounds observed in viable and non-viable hookworm ova were tetradecanoic acid, commonly known as myristic acid [fold change (FC) = 0.4], and dodecanoic acid, commonly known as lauric acid (FC = 0.388). Additionally, the viable ova had self-protecting metabolites such as prostaglandins, a typical feature absent in non-viable ova. The results of this study demonstrate that metabolic profiling using GC-MS methods can be used to determine the viability of canine hookworm ova. Further studies are needed to assess the applicability of metabolic profiling using GC-MS to detect viable hookworm ova in the mixed (viable and non-viable) populations from environmental samples and identify the metabolites specific to human hookworm species.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Gyawali P,Beale DJ,Ahmed W,Karpe AV,Magalhaes RJ,Morrison PD,Palombo EAdoi
10.1007/s00436-016-5112-4subject
Has Abstractpub_date
2016-09-01 00:00:00pages
3485-92issue
9eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5112-4journal_volume
115pub_type
杂志文章abstract：:Spermatogenesis, testes, and vas deferens in the parthenogenetic monozoic tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea) from Slovakia, parasitizing the carp Cyprinus carpio L., have been investigated by means of transmission electron microscopy for the first time. The present results sh...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2038-0
更新日期：2011-01-01 00:00:00
abstract：:Neodiplostomum americanum Chandler and Rausch, 1947 has been reported from six species of owls in North America. At present, there are no molecular data for this species and gene sequence data from Neodiplostomum Railliet, 1919 are limited. A freshly deceased specimen of the Great Horned Owl Bubo virginianus Gmelin, 1...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5503-1
更新日期：2017-08-01 00:00:00
abstract：:Mosquito repellents represent a cleaner and safer alternative for population control and reduce the diseases they carry in large areas of the world. Recently, research has been focused on repellents of natural origins, both crude essential oils and their main constituents. We have observed that, although a large numbe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3827-7
更新日期：2014-05-01 00:00:00
abstract：:Cryptosporidium spp. are common intestinal protozoa causing diarrhea in humans and a variety of animal species. With the recent development of fur industry, a large number of fur animals are farmed worldwide, especially in China. The existence of identical Cryptosporidium species/genotypes in humans and fur animals su...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5686-5
更新日期：2018-01-01 00:00:00
abstract：:With the accelerated movement of population, human parasitoses become an increasingly serious public health's problem. Currently, detections of parasite eggs through microscopic images are still the golden standard for diagnoses. However, this conventional method relies heavily on the experiences of inspectors, thus g...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4611-z
更新日期：2015-10-01 00:00:00
abstract：:Leishmania (L.) tropica is a causative agent of cutaneous and occasionally visceral or viscerotropic leishmaniasis in humans. The dose of parasites influences the course and outcome of disease in some Leishmania species. The effect of parasite dose on L. tropica infection in an experimental model was studied in the cu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5991-7
更新日期：2018-09-01 00:00:00
abstract：:A molecular approach was used to analyse a focus of cercarial dermatitis in northeastern France (Lake Der-Chantecoq), including both cercariae and snails,by sequencing the internal transcribed spacers (ITS1 for ocellate furcocercariae and ITS2 for snails). Lymnaea stagnalis were found infected with the furcocercariae ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1273-7
更新日期：2005-01-01 00:00:00
abstract：:The aims of this study were to examine specimens of Contracaecum rudolphii from Australian hosts, both morphologically and genetically, based on the ITS-1 and ITS-2 sequence data, to provide a re-description for the species and to investigate the genetic make-up of this species in Australia. Morphological examination ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1424-y
更新日期：2009-08-01 00:00:00
abstract：:The purpose of this paper is to disseminate, more widely, certain historically important aspects of research by O. Wilford Olsen on hookworms (Uncinaria lucasi) in northern fur seals (Callorhinus ursinus) and Steller sea lions (Eumatopias jubatus) in 1951 on St. Paul Island, Alaska. Highly meaningful facets of the res...
journal_title：Parasitology research
pub_type： 历史文章,杂志文章
doi：10.1007/s00436-005-1309-7
更新日期：2005-03-01 00:00:00
abstract：:This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of P...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2514-1
更新日期：2012-02-01 00:00:00
abstract：:Myxobolus miyairii Kudo, 1919 was first reported from the intestines of the Amur catfish (Silurus asotus) in Japan and then in China and Russia, but with incomplete description. During the investigation of fish myxosporean diversity in Poyang Lake, the biggest lake along the Yangtze River, China, two Amur catfish high...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4889-x
更新日期：2016-04-01 00:00:00
abstract：:Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the spe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3325-3
更新日期：2013-04-01 00:00:00
abstract：:The agar-gel migration technique has previously been described, however, aspects regarding the effect of timing on worm migration needed further scrutiny. In the first experiment, pigs inoculated with Oesophagostomum dentatum were slaughtered simultaneously and their intestines stored at 21-23 degrees C until processe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050405
更新日期：1998-01-01 00:00:00
abstract：:Morphology of capsules surrounding acanthocephalan Corynosoma strumosum in paratenic hosts (sea fishes of three species from the northern part of the Sea of Okhotsk) was studied. A thick layer of glycocalyx is formed on the surface of acanthocephalan's tegument in smelts Osmerus mordax dentex and Hypomesus olidus; the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2088-3
更新日期：2011-02-01 00:00:00
abstract：:While bivalves can acquire trematode metacercariae over their lifetime, the rate at which this accumulation takes place is not necessarily linear. The present study found that the bivalve Macomona liliana acquires very few or no metacercariae until it reaches 30 mm in size, but thereafter the rate at which it becomes ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1166-2
更新日期：2008-12-01 00:00:00
abstract：:The volatile metabolites of Greek wild growing Thymus leucospermus and Thymus teucrioides subsp. candilicus were determined by gas chromatography and gas chromatography-mass spectrometry. The monoterpene hydrocarbon p-cymene (64.2%) dominated T. leucospermus oil, followed by γ-terpinene (7.9%), thymol (4.8%), and born...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2271-1
更新日期：2011-08-01 00:00:00
abstract：:Cryptosporidium spp. and Giardia duodenalis are protozoan parasites that cause diarrhea in humans and animals. Molecular data on Cryptosporidium spp. and G. duodenalis in calves in the Republic of Korea (ROK) is limited; thus, we investigated the prevalence of Cryptosporidium and Giardia in pre-weaned calves, analyzed...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06482-9
更新日期：2019-12-01 00:00:00
abstract：:The chemical factors involved in oviposition site selection by mosquitoes have become the focus of interest in recent years, and considerable attention is paid to the chemical cues influencing mosquito oviposition. Studies on synthetic oviposition attractants/repellents of long-chain fatty acid esters against Anophele...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1189-8
更新日期：2009-01-01 00:00:00
abstract：:The vitellarium of the invasive caryophyllidean tapeworm Khawia sinensis Hsü, 1935 from carp Cyprinus carpio L. was examined by means of transmission electron microscopy and cytochemical staining for glycogen with periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP). A vitellarium consists of numerous follicl...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3477-1
更新日期：2013-07-01 00:00:00
abstract：:The viability of Cryptosporidium parvum oocysts ingested by Artemia franciscana metanauplii was evaluated using two fluorogenic vital dyes. There was no significant difference (p = 0.09) between the viability of oocysts maintained in saline (control) and those recovered from the digestive tract of the microcrustacean ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0543-6
更新日期：2007-08-01 00:00:00
abstract：:The apparent scarcity or absence of blood parasites in some avian groups, such as seabirds, has been related to intrinsic and extrinsic factors including host immunological capacity, host-parasite assemblage, and ecological parameters, but also to reduced sensitivity of some methods to detect low parasite prevalence/i...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-017-5676-7
更新日期：2018-01-01 00:00:00
abstract：:Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive s...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5343-4
更新日期：2017-02-01 00:00:00
abstract：:Several rhoptry proteins (ROPs) have been confirmed to be critical virulence factors of Toxoplasma gondii strains from North America and Europe. The two active kinases ROP17 and ROP18, and pseudokinase ROP5 were thought to be the key determinants of parasites' virulence in laboratory mice. Given the genetic diversity ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5141-z
更新日期：2016-10-01 00:00:00
abstract：:The present study reports on the antifilarial activity of a marine sponge Haliclona exigua (phylum Porifera). The crude methanol extract and n-butanol-soluble fraction killed adult Brugia malayi at 31.25-microg/ml concentration (both in motility and 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1555-1
更新日期：2009-10-01 00:00:00
abstract：:We assessed the cellular immunological responses to two Plasmodium falciparum liver-stage antigen (LSA)-1-derived T-cell epitopes in healthy Gabonese children and adults. The N-terminal peptide, designated T1, induced interferon (IFN)-gamma production in peripheral blood mononuclear cells (PBMC) from a significantly l...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0703-7
更新日期：2002-12-01 00:00:00
abstract：:Blastocytis sp. is the most common enteric protozoan in human, but its pathogenesis is still unclear. To study the infectious effects of Blastocytis sp. on tissue damage, we orally challenged the Sprague Dawley (SD) rats with different doses of Blastocytis subtype1 (ST1) and examined the histological changes. We found...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3536-7
更新日期：2013-10-01 00:00:00
abstract：:Sarcocystis neurona is the parasite associated with equine protozoal myeloencephalitis (EPM). Recently, cats (Felis domesticus) have been implicated as a potential intermediate host in the life cycle of S. neurona. This study was initiated to determine whether cats have antibodies that react to S. neurona antigens sim...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100503
更新日期：2002-01-01 00:00:00
abstract：:The objective of the study was to estimate the prevalence of helminths in the horse population of the state of Brandenburg, Germany. One hundred and twenty-six horse farms in the state were selected by randomised stratified sampling. In total, 1,407 horses across all farms were examined coproscopically. The experiment...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2362-z
更新日期：2011-05-01 00:00:00
abstract：:Both the oncospheral tegument and the hook region membrane (HRM) of Echinococcus multilocularis hexacanths originate from a syncytial binucleate complex that appears in the early stage of morphogenesis and organogenesis of the hexacanth larva. The primordium of this binucleate complex forms a binucleate syncytial cap ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5752-7
更新日期：2018-03-01 00:00:00
abstract：:Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF02351874
更新日期：1994-01-01 00:00:00
