Abstract:
:Differentiation between viable and non-viable hookworm ova in environmental samples is necessary in order to implement strategies to mitigate re-infections in endemic regions. In this study, an untargeted metabolic profiling method was developed that utilised gas chromatography-mass spectrometry (GC-MS) in order to investigate hookworm ova viability. Ancylostoma caninum was used to investigate the metabolites within viable and non-viable ova. Univariate and multivariate statistical analyses of the data resulted in the identification of 53 significant metabolites across all hookworm ova samples. The major compounds observed in viable and non-viable hookworm ova were tetradecanoic acid, commonly known as myristic acid [fold change (FC) = 0.4], and dodecanoic acid, commonly known as lauric acid (FC = 0.388). Additionally, the viable ova had self-protecting metabolites such as prostaglandins, a typical feature absent in non-viable ova. The results of this study demonstrate that metabolic profiling using GC-MS methods can be used to determine the viability of canine hookworm ova. Further studies are needed to assess the applicability of metabolic profiling using GC-MS to detect viable hookworm ova in the mixed (viable and non-viable) populations from environmental samples and identify the metabolites specific to human hookworm species.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Gyawali P,Beale DJ,Ahmed W,Karpe AV,Magalhaes RJ,Morrison PD,Palombo EAdoi
10.1007/s00436-016-5112-4subject
Has Abstractpub_date
2016-09-01 00:00:00pages
3485-92issue
9eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5112-4journal_volume
115pub_type
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