Parasitological and molecular diagnostic of a clinical Babesia caballi outbreak in Southern Romania.

Abstract:

:Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Babesia caballi and/or Theileria equi, which is endemic in many tropical and temperate areas of the world. However, clinical outbreaks of EP in Romania during the last decades have not been reported Therefore, the aim of this paper is (i) to describe a clinical B. caballi outbreak in horses on several farms in Southern Romania using a diagnostic and therapeutic approach and (ii) the molecular diagnostic of EP in an endemic area of Romania. In the first case, a 10-month-old stallion male was presented with lethargy, anorexia, fever (40.9 °C), pale mucosal/mucous/membranes and a marked anemia. In the subsequent weeks, three horses from other farms located in the same area, displayed similar clinical signs. B. caballi was diagnosed in all the horses based on Giemsa-stained blood smears and the diagnosis was further confirmed by polymerase chain reaction (PCR), using a single-round and multiplex PCR and sequencing. All four horses were treated with imidocarb dipropionate, at a dose rate of 2.2 mg/kg body weight (two injections at 48 h apart), and all horses clinically recovered within 24-48 h, post-treatment. This report presents the first molecularly characterized B. caballi outbreak in Romania in clinically affected horses, confirmed by DNA sequencing.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Ionita M,Nicorescu IM,Pfister K,Mitrea IL

doi

10.1007/s00436-018-5899-2

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

2333-2339

issue

7

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-018-5899-2

journal_volume

117

pub_type

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