Abstract:
:For decades, drug resistance has been the major obstacle in the fight against malaria, and the search for new drugs together with the combination therapy constitutes the major approach in responding to this situation. The present study aims at assessing the in vitro antimalarial activity of four compounds isolated from Kigelia africana stem bark (atranorin - KAE1, specicoside - KAE7, 2β,3β,19α-trihydroxy-urs-12-20-en-28-oic acid - KAE3, and p-hydroxy-cinnamic acid - KAE10) and their drug interactions among themselves and their combination effects with quinine and artemether. The antiplasmodial activity and drug interactions were evaluated against the multidrug-resistant W2mef strain of Plasmodium falciparum using the parasite lactate dehydrogenase assay. Three of the four compounds tested were significantly active against W2mef: specicoside (IC(50) = 1.02 ± 0.17 μM), 2β,3β,19α-trihydroxy-urs-12-en-28-oic acid (IC(50) = 1.86 ± 0.15 μM) and atranorin (IC(50) = 1.78 ± 0.18 μM), whereas p-hydroxy-cinnamic acid showed a weak activity (IC(50) = 12.89 ± 0.87 μM). A slight synergistic effect was observed between atranorin and 2β,3β,19α-trihydroxy-urs-12-en-28-oic acid (Combination index, CI = 0.82) whereas the interaction between specicoside and p-hydroxy-cinnamic acid were instead antagonistic (CI = 2.67). All the three compounds showed synergistic effects with artemether, unlike the slight antagonistic interactions of atranorin and 2β,3β,19α-trihydroxy-urs-12-en-28-oic acid in combination with quinine. K. africana compounds are therefore likely to serve as leads in the development of new partner drugs in artemether-based combination therapy.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Zofou D,Tene M,Tane P,Titanji VPdoi
10.1007/s00436-011-2519-9subject
Has Abstractpub_date
2012-02-01 00:00:00pages
539-44issue
2eissn
0932-0113issn
1432-1955journal_volume
110pub_type
杂志文章abstract::Cystic echinococcosis (CE) is a widespread zoonosis. For treating single echinococcal cysts during the last decades, therapeutic puncture of the cyst, aspiration, injection of a scolicide, and re-aspiration (PAIR) has been established as a minimal-invasive alternative method to surgery. A recent review on the complica...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-014-4275-0
更新日期:2015-02-01 00:00:00
abstract::Tetratrichomonas didelphidis is a flagellate protozoan found in the intestine of the opossum. The parasite lives in a hostile and stressed environment, where it interacts with microorganisms and can survive under extreme conditions for growth, involving strict anaerobiosis or equilibration with air and abundance or ab...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-003-1015-2
更新日期:2004-01-01 00:00:00
abstract::In this study, the antigenic mosaic of the vesicular fluid (VF) and hydrosoluble cyst-wall extract (CWE) of T. crassiceps (Tc; harvested from mice) and T. saginata (Ts) metacestodes was analyzed by combined precipitation maps developed in single and bidimensional immunoelectrophoresis against the respective rabbit ant...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931168
更新日期:1989-01-01 00:00:00
abstract::The hypersaline lagoons located in evaporation basins or salars (salt flats) in the Atacama Desert are extreme environments harbouring a specialised biota able to survive harsh conditions for life. The knowledge of the parasitic biodiversity of these extreme habitats is still scarce despite their functional importance...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06768-3
更新日期:2020-10-01 00:00:00
abstract::The complex life cycle of Toxoplasma gondii involves many animal species, raising zoonotic, economic, and conservation issues. This complexity is reflected in the molecular structure of T. gondii, whose different genotypes differ in pathogenicity. Among the intermediate hosts of T. gondii, wild ungulates may be a sour...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-4981-x
更新日期:2016-05-01 00:00:00
abstract::In this study, we compared and validated a nested ABC polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay to amplify Cryptosporidium parvum oocyst wall protein (COWP) gene fragment with a previous nested PCR-RFLP method. When RFLP of COWP gene did not provide clear results, we further a...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1233-8
更新日期:2009-02-01 00:00:00
abstract::The objectives of this study were to evaluate the occurrence of Leishmania infantum in the male and female genital tract and female mammary glands of dogs and the parasite burden and to identify histological alterations associated with this protozoan. Twenty male and 20 female Leishmania-seropositive dogs with isolati...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-4987-4
更新日期:2016-06-01 00:00:00
abstract::The complete coding sequence of manganese superoxide dismutase (Mn-SOD) of Trichinella pseudospiralis (Tp) was obtained and characterized for the first time by degenerative reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of complementary DNA ends (RACE) reactions. The open reading fram...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0755-9
更新日期:2008-01-01 00:00:00
abstract::Tetracapsuloides bryosalmonae (Myxozoa) is the causative agent of proliferative kidney disease in various species of salmonids which are found in Europe and North America. Less information about the interactions of T. bryosalmonae proteins with salmonid proteins during parasite development is known. In this study, ant...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4357-7
更新日期:2015-05-01 00:00:00
abstract::With this case report, we describe Trombiculidae larvae (Neotrombicula autumnalis) infestation in a Little Bittern (Ixobrychus minutus) which was brought to our clinics by the Directorship of Environmental and Forestry authorities of Samsun, Turkey in April, 2007. The male Little Bittern (I. minutus), with a black bac...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-0898-3
更新日期:2008-04-01 00:00:00
abstract::The time-course of the detection of circulating and urinary filarial antigens was followed with a 2S-IRMA assay, using a mouse monoclonal antibody raised against Brugia malayi larvae, in Mastomys natalensis experimentally infected with Brugia malayi, Brugia pahangi, or Litomosoides carinii. In the prepatent phase of t...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00536033
更新日期:1987-01-01 00:00:00
abstract::The diversity of ticks and tick-borne pathogens (TBPs) infesting domestic animals in Tchicala-Tcholoanga, Angola, in 2016 was investigated. Seventeen tick species were recorded, Amblyomma pomposum being the most abundant on cattle (40%), goats (38%) and sheep (35%); Rhipicephalus turanicus was the most abundant on dog...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-07009-3
更新日期:2021-01-08 00:00:00
abstract::Gas-liquid chromatographic studies were done to determine the fatty acid composition of the digestive gland-gonad (DGG) complex of Helisoma trivolvis snails infected with the intramolluscan stages of Echinostoma trivolvis, of rediae freed from the DGG, of uninfected DGG, and of 41-day-old adult worms grown in golden h...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931585
更新日期:1993-01-01 00:00:00
abstract::Microsporidia is a group of spore-forming microorganisms with zoonotic potential. This study aimed to compare intestinal microsporidia infections in cat owners and non-pet owners. In total, 210 fecal samples were collected from indoor cats, cat owners, and non-pet owners. DNA extraction was performed and the small sub...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-020-06690-8
更新日期:2020-06-01 00:00:00
abstract::In life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0834-y
更新日期:2008-04-01 00:00:00
abstract::Results obtained in experiments testing the efficacy of anti-procyclic-form rabbit sera on the development of homologous and heterologous stocks of Trypanosoma brucei brucei in Glossina morsitans morsitans indicated that this development was affected little, or not at all, by such sera. The absence of effect of anti-p...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00934382
更新日期:1991-01-01 00:00:00
abstract::Ras are key components of diverse signal transduction pathways and play important roles in growth and development. To know about growth regulation in Clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cDNA library. The open reading frame contains 561 bp ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-2147-9
更新日期:2011-04-01 00:00:00
abstract::Infectious agents, including parasites, often have oncogenic potential. However, there has been no study on the association between Strongyloides stercoralis infection and cancer risk. Therefore, we investigated the relationship between S. stercoralis infection and the occurrence of hepato-pancreato-biliary cancer. Th...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0648-y
更新日期:2007-10-01 00:00:00
abstract::The efficacy of abamectin 1%, when injected subcutaneously in cattle at a dose of 200 micrograms/kg body weight, against the larval stages (grubs) of the fly Dermatobia hominis was evaluated in two trials in endemic areas of Brazil and Argentina. Eighteen Holstein x Brahman castrated males and 16 Brahman-cross with na...
journal_title:Parasitology research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF00931889
更新日期:1993-01-01 00:00:00
abstract::The petroleum ether extract of Viola websteri Hemsl. (Violaceae) was investigated for its activity against chloroquine-sensitive strains of Plasmodium falciparum using the parasite lactate dehydrogenase assay method. The main antiplasmodial principles, 6-(8'Z-pentadecenyl)-salicylic acid (1) and 6-(8'Z, 11'Z, 14'Z-hep...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-008-1205-z
更新日期:2009-01-01 00:00:00
abstract::The widespread apicomplexan parasites Toxoplasma gondii (T. gondii) and Eimeria tenella (E. tenella) are important pathogens with high prevalence in poultry. The aim of our study was the investigation of mutual influences in co-infected chickens, focusing on immune response and course of infection. Two separate trials...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-017-5636-2
更新日期:2017-11-01 00:00:00
abstract::Lymphatic filariasis is mainly caused by the filarial nematodes Wuchereria bancrofti and Brugia malayi. Wolbachia, intracellular symbiotic bacteria in filarial parasite, is known to induce immune response predominantly through Toll-like receptor 2 (TLR2). This study was performed to investigate the association between...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-010-1932-9
更新日期:2010-09-01 00:00:00
abstract::Flame atomic absorption spectrometry was performed to determine the alteration of calcium concentration in the soft parts and shells of Biomphalaria alexandrina and Bulinus truncatus due to the infection with Schistosoma mansoni and S. haematobium, respectively. The results showed significant lowering in the calcium c...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-007-0566-z
更新日期:2007-09-01 00:00:00
abstract::Disseminated visceral coccidiosis (DVC) is a widely distributed intestinal and extraintestinal disease of cranes caused by eimeriid coccidia and has lethal pathogenicity to several crane species. Here, feces of 164 black-necked cranes collected in Dashanbao Black-necked Crane National Nature Reserve, China, were exami...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-5765-2
更新日期:2018-03-01 00:00:00
abstract::Some cyclophyllidean cestodes provide protection for their eggs in the external environment by providing them with additional protective layers around the egg membranes. In attempting to examine such adaptations, the microanatomy and fine structure of the uterus of pregravid and gravid proglottids of the cyclophyllide...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-016-4997-2
更新日期:2016-06-01 00:00:00
abstract::Tick-borne intracellular protozoan parasites of the Theileria genus infect a wide range of both domestic and wild animals. In the present study, we describe the first PCR detection of Theileria luwenshuni in the blood of goats in Myanmar. Nested PCR targeting the Theileria 18S rRNA gene resulted in seven positive goat...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-018-6073-6
更新日期:2018-10-01 00:00:00
abstract::Two hundred forty feral domestic cats trapped between 2004 and 2008 in the city centre and the suburb districts of Dubai, as well as in desert biotopes, were subjected to a complete parasitological dissection. The established parasite fauna consisted of Cystoisospora felis (12.9%), Cystoisospora rivolta (9.2%), Toxopl...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-009-1372-6
更新日期:2009-07-01 00:00:00
abstract::In an experiment including 8 groups of 15 mice, the effect of migrating Ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to Pasteurella multocida was investigated. Following aerosol exposure to P. multocida, mice with migrating A. suum in their lungs developed more severe pne...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00931575
更新日期:1992-01-01 00:00:00
abstract::The acanthocephalan parasite Acanthocephalus dirus infects the freshwater isopod Caecidotea intermedius as an intermediate host before completing its life cycle in a fish. Transmission to the definitive host occurs after the parasite has reached the cystacanth stage, and development into this stage is associated with ...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/s00436-015-4753-z
更新日期:2016-01-01 00:00:00
abstract::Dimethyl sulfoxide (DMSO), commonly used for cryoprotection or for the solvation of cytoskeleton-modifying drugs, causes changes in the topology of the plasma membrane of the tegumental brush border in the tapeworm Hymenolepis diminuta; however, relatively long exposures of high concentrations are required. In tapewor...
journal_title:Parasitology research
pub_type: 杂志文章
doi:10.1007/BF00936184
更新日期:1992-01-01 00:00:00