Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae).

Abstract:

:The tick Rhipicephalus microplus affects cattle health, with production loss in tropical and subtropical regions. Moreover, the use of commercial acaricides has been reduced due to the resistance of this parasite. Although alternatives such as plant bioactive molecules have been sought, essential oils present variations in their chemical constituents due to environmental factors, which can interfere with their acaricidal activity. The objective of the present study was to evaluate the seasonal influence of the essential oil of Ocimum gratissimum and its major constituents on acaricidal activity against R. microplus larvae. A high-yield essential oil of O. gratissimum and its major constituents were used, and a plant with a thymol-type oil was selected for seasonal analysis and acaricidal activity against R. microplus. Gas chromatography (GC) and GC-mass spectrometry (MS) were employed to identify 31 oil constituents (average yield of 6.26%). The main compounds were found to be thymol (33.4 to 47.9%), γ-terpinene (26.2 to 36.8%), and p-cymene (4.3 to 17.0%). Concerning acaricidal activity, the December (LC50 0.84 mg/mL) and September (LC50 1.58 mg/mL) oils obtained in the dry season were the most active, and assays performed with commercial standards revealed LC50 values of p-cymene, thymol, and γ-terpinene of 1.41, 1.81, and 3.08 mg/mL, respectively. Overall, lower acaricidal activities were found for oils produced from plants harvested in the rainy season. The results showed that seasonal variation in the chemical composition of the O. gratissimum essential oil influences its acaricidal activity. The seasonal variations in the thymol-type essential oil of O. gratissimum can represent an important strategy for the control of R. microplus.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Silva Lima A,Milhomem MN,Santos Monteiro O,Arruda ACP,de Castro JAM,Fernandes YML,Maia JGS,Costa-Junior LM

doi

10.1007/s00436-017-5662-0

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

59-65

issue

1

eissn

0932-0113

issn

1432-1955

pii

10.1007/s00436-017-5662-0

journal_volume

117

pub_type

杂志文章
  • In vitro antiplasmodial activity and cytotoxicity of crude extracts and compounds from the stem bark of Kigelia africana (Lam.) Benth (Bignoniaceae).

    abstract::In order to assess the potential of the stem bark of Kigelia africana (Lam.) Benth as source of new anti-malarial leads, n-hexane and ethyl acetate (EtOAc) extracts and four compounds isolated from the stem bark were screened in vitro against the chloroquine-resistant W-2 and two field isolates of Plasmodium falciparu...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2363-y

    authors: Zofou D,Kengne AB,Tene M,Ngemenya MN,Tane P,Titanji VP

    更新日期:2011-06-01 00:00:00

  • Effect of polyclonal anti-procyclic antibodies on development of Trypanosoma brucei brucei in tsetse flies.

    abstract::Results obtained in experiments testing the efficacy of anti-procyclic-form rabbit sera on the development of homologous and heterologous stocks of Trypanosoma brucei brucei in Glossina morsitans morsitans indicated that this development was affected little, or not at all, by such sera. The absence of effect of anti-p...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00934382

    authors: Honigberg BM,Hampton RW,Cunningham I

    更新日期:1991-01-01 00:00:00

  • Experimental infections with Fasciola in snails, mice and rabbits.

    abstract::Experimental infection trails of Lymnaea (cailliaudi) natalensis snails with miracidia of Fasciola hepatica revealed neither cercariae nor larval stages shed. Infection of white mice with metacercariae from field-collected snails proved to be negative for Fasciola eggs and immature juveniles or adults after 84 days po...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-008-0888-5

    authors: Hussein AN,Khalifa RM

    更新日期:2008-05-01 00:00:00

  • Comparative efficacy of trichlorphon and trichlorphon/ivermectin combination treatment against anthelmintic-resistant cattle nematodes in Argentina.

    abstract::Twenty-four Holando Argentino male calves were treated orally with 50 mg/kg body weight trichlorphon (TCF); 0.2 mg/kg body weight subcutaneous ivermectin (IVM); a combination of TCF+IVM at the same doses and administration routes; or remained untreated (control group). All calves were necropsied at day 14 post treatme...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-011-2407-3

    authors: Fiel C,Guzmán M,Steffan P,Prieto O,Bhushan C

    更新日期:2011-08-01 00:00:00

  • Molecular characterization of Echinococcus granulosus isolates from Bulgarian human cystic echinococcosis patients.

    abstract::Although cystic echinococcosis (CE) is highly endemic in Bulgaria, there is still scarce information about species and/or genotypes of the Echinococcus granulosus complex that infect humans. Our study tackled the genetic diversity of E. granulosus complex in a cohort of 30 Bulgarian CE patients. Ten animal E. granulos...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-017-5386-1

    authors: Marinova I,Spiliotis M,Wang J,Muhtarov M,Chaligiannis I,Sotiraki S,Rainova I,Gottstein B,Boubaker G

    更新日期:2017-03-01 00:00:00

  • Larvicidal activity of lipophilic extract of Hypericum carinatum (Clusiaceae) against Aedes aegypti (Diptera: Culicidae) and benzophenones determination.

    abstract::In this study, the larvicidal activity of an enriched fraction of the major lipophilic phenolic compounds from Hypericum carinatum Griseb. (Clusiaceae) was investigated against larvae of Aedes aegypti (Diptera: Culicidae), the main vector of dengue virus in Brazil. The larval mortality rate ranged 37.33 to 72.00 % at ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3401-8

    authors: da Silva FC,de Barros FM,Prophiro JS,da Silva OS,Pereira TN,de Loreto Bordignon SA,Eifler-Lima VL,von Poser GL

    更新日期:2013-06-01 00:00:00

  • Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease.

    abstract::The purpose of this study was to evaluate morphologically the tongue of individuals with chronic Chagas disease (CD) in comparison to the non-chagasic ones. Twenty-four protocol cases of autopsies were selected. They were subdivided into CD patients (10 cases) and non-chagasic ones (14 cases). The morphometric analysi...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-005-0023-9

    authors: de Lima Pereira SA,Rodrigues DB,da Fonseca Ferraz ML,da Cunha Castro EC,dos Reis MA,de Paula Antunes Teixeira V

    更新日期:2006-01-01 00:00:00

  • The rabbit coccidium Eimeria flavescens Marotel and Guilhon, 1941: an electron microscopic study of its life cycle.

    abstract::The endogenous cycle of Eimeria flavescens was studied in specific pathogen-free rabbits by means of histology and transmission electron microscopy. In total, five asexual generations were observed and two types of meronts and merozoites were found in each generation. Type A gave rise to a smaller number of thick poly...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-003-0946-y

    authors: Pakandl M,Cerník F,Coudert P

    更新日期:2003-10-01 00:00:00

  • Trypanosoma cruzi-secreted vesicles have acid and alkaline phosphatase activities capable of increasing parasite adhesion and infection.

    abstract::Trypanosoma cruzi virulence factors include molecules expressed on the cell surface as well as those secreted or shed into the extracellular medium. Phosphatase activities modulate different aspects of T. cruzi infection, although no studies to date addressed the presence and activity of phosphatases in vesicles secre...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-014-3958-x

    authors: Neves RF,Fernandes AC,Meyer-Fernandes JR,Souto-Padrón T

    更新日期:2014-08-01 00:00:00

  • Status and origin of Haemonchinae (Nematoda: Trichostrongylidae) in deer: a survey conducted in France from 1985 to 1998.

    abstract::During our investigations on helminthofauna in roe deer (Capreolus capreolus) and red deer (Cervus elaphus) in France (1985-1998) we isolated nematodes not only of the genus Haemonchus Cobb, 1898 but also of the genus Ashworthius Le Roux 1930, both of which belong to the same subfamily of Haemonchinae. The prevalence ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/pl00008534

    authors: Ferté H,Cléva D,Depaquit J,Gobert S,Léger N

    更新日期:2000-07-01 00:00:00

  • Fine structure of uterus and non-functioning paruterine organ in Orthoskrjabinia junlanae (Cestoda, Cyclophyllidea).

    abstract::Some cyclophyllidean cestodes provide protection for their eggs in the external environment by providing them with additional protective layers around the egg membranes. In attempting to examine such adaptations, the microanatomy and fine structure of the uterus of pregravid and gravid proglottids of the cyclophyllide...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-4997-2

    authors: Korneva JV,Kornienko SA,Jones MK

    更新日期:2016-06-01 00:00:00

  • Phytochemical profile and larvicidal properties of seed essential oil from Nigella sativa L. (Ranunculaceae), against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae).

    abstract::The present study deals with investigation of larvicidal activity and their chemical constituents of the essential oil from the seeds of Nigella sativa L. (Ranunculaceae). Totally, 18 chemical compounds were identified by GC and GC-MS analysis. Thymol (19.13 %) and α-phellandrene (14.9 %) were identified as major chem...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4563-3

    authors: Raj GA,Chandrasekaran M,Krishnamoorthy S,Jayaraman M,Venkatesalu V

    更新日期:2015-09-01 00:00:00

  • Nuclear and mitochondrial genes for inferring Trichuris phylogeny.

    abstract::Nucleotide sequences of the triose phosphate isomerase (TPI) gene (624 bp) and mitochondrial cytochrome b (cob) gene (520 bp) were obtained by PCR and evaluated for utility in inferring the phylogenetic relationships among Trichuris species. Published sequences of one other nuclear gene (18S or SSU rRNA, 1816-1846 bp)...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4705-7

    authors: Callejón R,Cutillas C,Nadler SA

    更新日期:2015-12-01 00:00:00

  • A new avian Cryptosporidium genotype in a 1-month-old caged brown wood owl (Strix leptogrammica) with severe dehydration and diarrhea.

    abstract::A 1-month-old brown wood owlet (Strix leptogrammica) purchased from a wholesaler and housed as a companion bird by an individual owner in Japan showed severe dehydration and anorexia following a week of vomiting and severe diarrhea. A great number of approximately 5 × 4-μm-sized Cryptosporidium oocysts were found in t...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-018-5951-2

    authors: Makino I,Inumaru M,Abe N,Sato Y

    更新日期:2018-09-01 00:00:00

  • Characterization and bioassay for larvicidal activity of Anacardium occidentale (cashew) shell waste fractions against dengue vector Aedes aegypti.

    abstract::Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of A...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4598-5

    authors: Torres RC,Garbo AG,Walde RZ

    更新日期:2015-10-01 00:00:00

  • Use of triclabendazole for treatment of patients co-infected by Fasciola spp. and S. mansoni in Behera Governorate, Egypt.

    abstract::Praziquantel is still very effective for the treatment of schistosomiasis, but there are rising concerns on the potential risk of developing resistances because of the extensive use of this drug. Triclabendazole, a systemic anthelmintic, is very effective against other trematodes such as Paragonimus spp. and Fasciola ...

    journal_title:Parasitology research

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00436-007-0802-6

    authors: Barduagni P,Hassanein Y,Mohamed M,Wakeel AE,Sayed ME,Hallaj Z,Curtale F

    更新日期:2008-03-01 00:00:00

  • Experimental chemotherapy of Schistosoma mansoni with praziquantel and oxamniquine: differential effect of single or combined formulations of drugs on various strains and on both sexes of the parasite.

    abstract::The susceptibility of two Venezuelan (YT and SM) and one Brazilian (BH) strain of Schistosoma mansoni to single oral doses of praziquantel (Pz; 250 or 500 mg/kg), oxamniquine (Ox; 40, 60, or 100 mg/kg) or to low-dose combinations of both drugs (33 mg/kg Pz and 25 mg/kg Ox; 66 mg/kg Pz and 12.5 mg/kg Ox; 250 mg/kg Pz a...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/BF00931515

    authors: Delgado VS,Suárez DP,Cesari IM,Incani RN

    更新日期:1992-01-01 00:00:00

  • Mattesia weiseri sp. nov., a new neogregarine (Apicomplexa: Lipotrophidae) pathogen of the great spruce bark beetle, Dendroctonus micans (Coleoptera: Curculionidae, Scolytinae).

    abstract::A new neogregarine pathogen of the great spruce bark beetle, Dendroctonus micans (Coleoptera: Curculionidae, Scolytinae), is described based on light microscopy and ultrastructural characteristics. The pathogen infects the fat body and the hemolymph of the beetle. The infection was nonsynchronous so that different dev...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-015-4497-9

    authors: Yaman M,Radek R

    更新日期:2015-08-01 00:00:00

  • Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique.

    abstract::The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clo...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-016-5249-1

    authors: Galaktionov NK,Podgornaya OI,Strelkov PP,Galaktionov KV

    更新日期:2016-12-01 00:00:00

  • Thelohania montirivulorum sp. nov. (Microspora: Thelohaniidae), a parasite of the Australian freshwater crayfish, Cherax destructor (Decapoda: Parastacidae): fine ultrastructure, molecular characteristics and phylogenetic relationships.

    abstract::Thelohania montirivulorum sp. nov., a new species of microsporidian parasite, was found in a highland population of the Australian yabby, Cherax destructor. Data are presented on fine ultrastructure, developmental morphology and DNA sequence of the small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-003-0948-9

    authors: Moodie EG,Le Jambre LF,Katz ME

    更新日期:2003-10-01 00:00:00

  • Alien parasites on an alien fish species: monogeneans from the black bullhead Ameiurus melas (Siluriformes) in the Lake Srebarna Biosphere Reserve, Bulgaria, with the first record of Gyrodactylus nebulosus in the Palaearctic.

    abstract::Black bullhead Ameiurus melas (Actinopterygii: Siluriformes) is an alien fish species of North American origin, which has expanded its invasive geographical range in Europe. In 2017-2019, 32 black bullhead specimens from the Lake Srebarna Biosphere Reserve, Bulgaria, were examined for monogenean parasites. Two species...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-020-06699-z

    authors: Vancheva N,Bobeva A,Pehlivanov L,Stefanov T,Georgiev BB

    更新日期:2020-07-01 00:00:00

  • Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle.

    abstract::The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0810-6

    authors: Moré G,Basso W,Bacigalupe D,Venturini MC,Venturini L

    更新日期:2008-03-01 00:00:00

  • Effect of time on migration of Oesophagostomum spp. and Hyostrongylus rubidus out of agar-gel.

    abstract::The agar-gel migration technique has previously been described, however, aspects regarding the effect of timing on worm migration needed further scrutiny. In the first experiment, pigs inoculated with Oesophagostomum dentatum were slaughtered simultaneously and their intestines stored at 21-23 degrees C until processe...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s004360050405

    authors: Nosal P,Christensen CM,Nansen P

    更新日期:1998-01-01 00:00:00

  • A tool for diagnosis of Dicrocoelium dendriticum infection: hatching eggs and molecular identification of the miracidium.

    abstract::DNA primers were designed from the 18S rRNA sequence from the relevant digenean trematode Dicrocoelium dendriticum to evaluate a polymerase chain reaction-based diagnostic method of this parasite from its eggs in faeces of naturally and experimentally infected sheep. In order to get DNA from D. dendriticum eggs, sever...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3313-7

    authors: Sandoval H,Manga-González MY,Castro JM

    更新日期:2013-04-01 00:00:00

  • Efficacy of dinotefuran, permethrin and pyriproxyfen combination spot-on on dogs against Phlebotomus perniciosus and Ctenocephalides canis.

    abstract::This study was conducted to evaluate the efficacy of a new topical ectoparasiticidal spot-on containing 4.95% dinotefuran (w/w), 36.08% permethrin (w/w) and 0.44% pyriproxyfen (w/w) (Vectra 3D, Ceva, Libourne, France) against Portuguese strain of Phlebotomus perniciosus and a French strain of Ctenocephalides canis in ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3568-z

    authors: Liénard E,Bouhsira E,Jacquiet P,Warin S,Kaltsatos V,Franc M

    更新日期:2013-11-01 00:00:00

  • Evaluation of antileishmanial potential of Tinospora sinensis against experimental visceral leishmaniasis.

    abstract::The chemotherapeutic interventions against visceral leishmaniasis (VL) are limited and facing serious concerns of toxicity, high cost, and emerging drug resistance. There is a greater interest in new drug developments from traditionally used medicinal plants which offers unprecedented diversity in structures and bioac...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-007-0822-2

    authors: Singh N,Kumar A,Gupta P,Chand K,Samant M,Maurya R,Dube A

    更新日期:2008-02-01 00:00:00

  • Borrelia burgdorferi infection prevalences in questing Ixodes ricinus ticks (Acari: Ixodidae) in urban and suburban Bonn, western Germany.

    abstract::From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket dragging at 45 sites all over the city area of Bonn, western Germany, to be checked for Borrelia burgdorferi infection. The collection sites included 20 private gardens, nine ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-004-1240-3

    authors: Maetzel D,Maier WA,Kampen H

    更新日期:2005-01-01 00:00:00

  • Development of a PCR approach for differentiation of Alaria spp. mesocercariae.

    abstract::To date classification and differentiation of Alaria spp. are based largely on external characteristics and comparative morphology of adult flukes. The accurate differentiation between various Alaria spp. mesocercariae is indeed difficult because there are only few data on morphological and morphometrical features of ...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-010-2240-0

    authors: Riehn K,Hamedy A,Alter T,Lücker E

    更新日期:2011-05-01 00:00:00

  • Three actinosporean types (Myxozoa) from the oligochaete Branchiura sowerbyi in China.

    abstract::Three actinosporean types--raabeia, aurantiactinomyxon, and guyenotia--from the oligochaete Branchiura sowerbyi Beddard collected from a crucian carp (Carassius auratus gibelio) pond are described in this report. Compared with the actinospores described previously, the raabeia type presents similar spore shape with th...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-013-3306-6

    authors: Xi BW,Zhang JY,Xie J,Pan LK,Xu P,Ge XP

    更新日期:2013-04-01 00:00:00

  • Protective immunity against Toxoplasma gondii in mice induced by the SAG2 internal image of anti-idiotype antibody.

    abstract::A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 inte...

    journal_title:Parasitology research

    pub_type: 杂志文章

    doi:10.1007/s00436-003-1006-3

    authors: Yang CD,Chang GN,Chao D

    更新日期:2003-12-01 00:00:00