Effect of time on migration of Oesophagostomum spp. and Hyostrongylus rubidus out of agar-gel.

Abstract:

:The agar-gel migration technique has previously been described, however, aspects regarding the effect of timing on worm migration needed further scrutiny. In the first experiment, pigs inoculated with Oesophagostomum dentatum were slaughtered simultaneously and their intestines stored at 21-23 degrees C until processed pairwise 2, 4, 6, 8, 12 and 18 h after slaughter. More than 95% of the worms migrated out of the agar if processed within 6 h. In the second experiment, intestines were treated immediately after slaughter and the migratory speed of adult worms or 4th-stage larvae of O. dentatum or O. quadrispinulatum, or adult Hyostrongylus rubidus were studied. For both Oesophagostomum species, more than 90% of the worms were recovered within 1 h. H. rubidus was significantly slower; however, approximately 98% of the worms had migrated out of the agar-gel by 20 h. This information is essential in planning experiments where recovery of live worms is of value.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Nosal P,Christensen CM,Nansen P

doi

10.1007/s004360050405

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

328-32

issue

4

eissn

0932-0113

issn

1432-1955

journal_volume

84

pub_type

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